RETRACTED: Toward an Optimal Oligosaccharide Ligand for Rat Natural Killer Cell Activation Receptor NKR-P1

Synthesis of LacdiNAc-terminated glycoconjugates by mutant galactosyltransferase - A way to new glycodrugs and materials

“…It follows from our previous studies (Krist et al 2001;Křen 2003) that aminosugars are strong ligands of these activation receptors, with the possible application in in vivo stimulation of natural killer cells, especially due to the presence of the thiourea linkage, which is stable in vivo. The binding assays were essentially performed as described previously (Krist et al 2001;Fialová et al 2005), with fluorescently or radioactively labeled protein receptors, rat NKR-P1 and human CD69. D-Mannose served as a negative (noninhibitory) control carbohydrate and GlcNAc as a positive control, providing IC 50 values of 1.7 × 10 −5 M (NKR-P1A) and 1.0 × 10 −3 M (CD69).…”

Stereoselective entry into the d-GalNAc series starting from the d-Gal one: a new access to N-acetyl-d-galactosamine and derivatives thereof

“…5 Furthermore, D-GalNAc has been recently recognised as one of the strongest agonist of the NKR-P1 rat Natural Killer cells receptor. 6 Owing to the biological relevance of D-GalNAc, several approaches to its synthesis in the free form and/or to the synthesis of its derivatives have been proposed, using different carbohydrate starting materials. The two most exploited synthetic channels to D-GalNAc and its derivatives are those based (a) on the C-4 epimerization of the largely and cheaply available 7 mimicking thus the biosynthetic pathway, and (b) on the amination at C-2 of D-galactose with formal retention of configuration.…”

A New Preparation of the Disaccharide β‐D‐ManNAcp‐(1 → 4)‐D‐Glc from Lactose Through a Highly Stereoselective β‐D‐Galpto β‐D‐ManNAcpTransformation

“…[2,3] The b-D-ManNAcp residue is a component of the natural spacer unit linking teichoic acids to the peptidoglycan chain in some Gram-positive bacteria. [4] More recently, Kren and co-workers [5] demonstrated that ManNAc is the strongest 2-acetamido-2-deoxyhexose ligand for the natural killer cell activating protein (NKR-P1). [5] Furthermore, disaccharides containing ManNAc unit at the reducing end were proved to have the highest affinity for the NKR-P1 receptors.…”