Retraction

Abstract

Diabetes, is the eighth cause of death worldwide, is a kind of metabolic disease. We planned to study the potentials of the angelica polysaccharide (AP) in MIN6 cell line. Cells were induced with tumor necrosis factor α (TNF-α) dilution or APsolutions, and transfected with microRNA-143 (miR-143) as well as the corresponding negative control (NC). Viability was assessed via CCK-8, while the apoptosis rate was tested by the Annexin V-FITC/PI as well as flow cytometer. The insulin, miR-143 and relative proteins and the inducible nitric oxide synthase (iNOS) were individually determined via GSIS assay, qRT-PCR, and western blot. TNF-α induced inhibition of viability and secretion of insulin, but promotion of apoptosis and iNOS, as well as NF-κB and p38 MAPK cascades on MIN6 cell line. AP released TNF-α-resulted damage by up-regulating miR-143. miR-143 inhibitor partly abolished the influence of AP on MIN6 cells. AP released TNF-α-induced injury by promoting miR-143 through blocking NF-κB and p38 MAPK in the MIN6 cells.

Research Progress on the Mechanism of Action of Danggui-Shaoyao-San Polysaccharide on Some Complications of Type II Diabetes Mellitus: A Review

“…It has been shown that pachyman in P-DSS can activate PPAR by inhibiting p38 MAPK phosphorylationγ The pathway protects the kidney damage of model mice, and Western blot results show that with the increase of pachyman concentration, the level of p-p38 MAPK in kidney tissue gradually decreases, PPAR-γ The level rises gradually [90]. In addition, Angelica polysaccharide blocks NF in MIN6 cells-κ B and p38 MAPK to promote miR-143 and release TNF-α Induced damage, TNF-α Induction can inhibit pancreatic insulin secretion and reduce insulin activity [91]. The drug "Poria cocos Alisma" can activate the expression of AMPK signal pathway and inhibit its downstream targets ACC, SREBP-1C, PCSK9, PPAR γ、 HMGCR expression affects cholesterol and fatty acid metabolism [92].…”