1974
DOI: 10.1128/jb.117.3.1306-1311.1974
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Retraction of F Pili

Abstract: The disappearance of F pili on Escherichia coli cells in the presence of 10-2 M NaCN was studied by electron microscopy and serum-blocking power. The pili which disappeared from the cell did not appear as free pili in the culture medium, suggesting that the pili had retracted into the cell. New pili were produced at a normal rate approximately 3 min after NaCN was removed. The adsorption of either F pili antibody or R17 bacteriophage to the sides of pili and temperatures below 24 C prevented retraction. The di… Show more

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Cited by 88 publications
(53 citation statements)
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“…Type IV pili are divided into two major subfamilies, type IVa (T4aP) and type IVb (T4bP) pili, characterized by differences in the major and minor pilin subunits which make up the pilus fibre and among assembly system components (Burrows, ; Craig et al ., ). Unlike most other bacterial surface filaments with the exception of F‐pili (Novotny et al ., ), T4aP are dynamic and can be rapidly retracted, producing forces in excess of 100 pN per filament (Merz et al ., ; Maier et al ., ). Through their ability to repeatedly extend, adhere and retract, T4aP confer unique locomotion modalities including twitching, swarming, walking and sling shot motilities (Yeung et al ., ; Gibiansky et al ., ; Jin et al ., ).…”
Section: Introductionmentioning
confidence: 99%
“…Type IV pili are divided into two major subfamilies, type IVa (T4aP) and type IVb (T4bP) pili, characterized by differences in the major and minor pilin subunits which make up the pilus fibre and among assembly system components (Burrows, ; Craig et al ., ). Unlike most other bacterial surface filaments with the exception of F‐pili (Novotny et al ., ), T4aP are dynamic and can be rapidly retracted, producing forces in excess of 100 pN per filament (Merz et al ., ; Maier et al ., ). Through their ability to repeatedly extend, adhere and retract, T4aP confer unique locomotion modalities including twitching, swarming, walking and sling shot motilities (Yeung et al ., ; Gibiansky et al ., ; Jin et al ., ).…”
Section: Introductionmentioning
confidence: 99%
“…1 and 2 ; Table 1) and transfer DNA. Conjugation is thought to be initiated by contact between the F-pilus and a suitable recipient resulting in pilus retraction [12] and stable mating pair or aggregate formation [9]. Prior to the initiation of DNA transfer, the relaxosome, consisting of proteins bound to the origin of transfer (oriT), resides within the cytoplasm of donor cells [13].…”
Section: Introductionmentioning
confidence: 99%
“…It is further suggested that twitching motility may be caused by the ability of the P. aeruginosa pilus to extend and retract repeatedly (Bradley, 1972b;Bradley, 1980). A pilus retraction mechanism has also been proposed to account for the Escherichia coti F-factormediated formation of mating pairs and entry of a number of pitus-specific bacteriophages (Marvin and Hohn, 1969;Jacobson, 1972;Novotny and Fives-Taylor, 1974;O'Callaghan et ai, 1973). However, there are no known reports of F* cells that are capable of twitching motility.…”
mentioning
confidence: 99%