Retraction: Ultrahighly Sensitive Homogeneous Detection of DNA and MicroRNA by Using Single‐Silver‐Nanoparticle Counting

Xu, Fagong; Dong, Chuan; Xie, Changsheng; Ren, Jicun

doi:10.1002/chem.201090242

Cited by 4 publications

(4 citation statements)

References 6 publications

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“…Chemical reagents: Aniline (AN), 5-sulfo-2-methoxyaniline (SA), ammonium persulfate, AgNO 3 2 , and BaCl 2 of analytical-reagent grade were commercially obtained and used as received.…”

Section: Agmentioning

confidence: 99%

“…Silver is a precious metal used worldwide as currency and in functional nanomaterials for antimicrobial activity, [1] DNA and microRNA detection, [2] multicolor photochromism, [3] color luminescence, [4,5] self-erasing multicolor imaging, [6] catalytic activity, and optoelectronic effects. [7] Compared with early inefficient methods, modern techniques for silver recovery from scrap and wastewaters such as photographic films, X-ray films, and jewelry are highly efficient, and include sorption, precipitation, ion exchange, reductive exchange, and electrolysis.…”

Section: Introductionmentioning

confidence: 99%

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Redox Sorption and Recovery of Silver Ions as Silver Nanocrystals on Poly(aniline‐co‐5‐sulfo‐2‐anisidine) Nanosorbents

Feng

Huang

2010

Chemistry A European J

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Poly[aniline(AN)-co-5-sulfo-2-anisidine(SA)] nanograins with rough and porous structure demonstrate ultrastrong adsorption and highly efficient recovery of silver ions. The effects of five key factors-AN/SA ratio, Ag(I) concentration, sorption time, ultrasonic treatment, and coexisting ions-on Ag(I) adsorbability were optimized, and AN/SA (50/50) copolymer nanograins were found to exhibit much stronger Ag(I) adsorption than polyaniline and all other reported sorbents. The maximal Ag(I) sorption capacity of up to 2034 mg g(-1) (18.86 mmol g(-1)) is the highest thus far and also much higher than the maximal Hg-ion sorption capacity (10.28 mmol g(-1)). Especially at or=99.98 % adsorptivity, and thus achieve almost complete Ag(I) sorption. The sorption fits the Langmuir isotherm well and follows pseudo-second-order kinetics. Studies by IR, UV/Vis, X-ray diffraction, polarizing microscopy, centrifugation, thermogravimetry, and conductivity techniques showed that Ag(I) sorption occurs by a redox mechanism mainly involving reduction of Ag(I) to separable silver nanocrystals, chelation between Ag(I) and -NH-/-N=/-NH(2)/-SO(3)H/-OCH(3), and ion exchange between Ag(I) and H(+) on -SO(3) (-)H(+). Competitive sorption of Ag(I) with coexisting Hg, Pb, Cu, Fe, Al, K, and Na ions was systematically investigated. In particular, the copolymer nanoparticles bearing many functional groups on their rough and porous surface can be directly used to recover and separate precious silver nanocrystals from practical Ag(I) wastewaters containing Fe, Al, K, and Na ions from Kodak Studio. The nanograins have great application potential in the noble metals industry, resource reuse, wastewater treatment, and functional hybrid nanocomposites.

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“…Chemical reagents: Aniline (AN), 5-sulfo-2-methoxyaniline (SA), ammonium persulfate, AgNO 3 2 , and BaCl 2 of analytical-reagent grade were commercially obtained and used as received.…”

Section: Agmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Redox Sorption and Recovery of Silver Ions as Silver Nanocrystals on Poly(aniline‐co‐5‐sulfo‐2‐anisidine) Nanosorbents

Feng

Huang

2010

Chemistry A European J

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“…Nevertheless, its sensitivity and specificity should also be improved. − RT-qPCR has been proposed for miRNA analysis, and the sensitivity of miRNA detection can reach the single molecule level . However, it requires precise control of temperature cycling for successful amplification, and the short length of miRNAs makes their experimental design very sophisticated. − Except these standard methods for miRNA detection mentioned above, various new strategies have been developed to improve the detection sensitivity and adaptability, such as nanopartical-based assay, − bioluminescence-based assay, modified invader assay, ribozyme-based assay, sequencing-based assay, EXPAR assay, strand displacement assay, and hairpin-based amplification . Among these methods, rolling-circle amplification (RCA) has become increasingly popular in miRNA detection due to its simplicity, specificity, and high sensitivity. − After RCA-based miRNA detection was first reported, several novel strategies were developed to improve the specificity and sensitivity of this method by introducing a second primer, a dumbbell-shaped DNA probe, , DNAzyme, or encoded gel microparticles .…”

mentioning

confidence: 99%

High Specific and Ultrasensitive Isothermal Detection of MicroRNA by Padlock Probe-Based Exponential Rolling Circle Amplification

et al. 2013

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In this paper, a padlock probe-based exponential rolling circle amplification (P-ERCA) assay is developed for highly specific and sensitive detection of microRNA (miRNA). The padlock probe is composed of a hybridization sequence to miRNA and a nicking site for nicking endonuclease. Using the miRNA as a template, specific ligation to the padlock probe and linear rolling circle reaction (LRCA) are achieved under isothermal conditions. After multiple nicking reactions, many copies of short DNA products are successively produced and then used as triggers in next circle amplification. Thus, a small amount of miRNAs are converted to a large number of triggers to initiate the rolling circle amplification reaction, and circular exponential signal amplification is achieved. This padlock probe-based exponential rolling circle amplification assay exhibits a remarkable sensitivity of 0.24 zmol using optimized sequences of the padlock probe. The target-dependent circularization of the padlock probe and the ligation reaction could improve the specificity effectively, leading to single-nucleotide difference discrimination between miRNA family members. The miRNA analysis in human lung cells was performed with this method. The result indicates this highly sensitive P-ERCA strategy will become a promising miRNA quantification method in early clinical diagnostics.

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“…Owing to the high affinity, easy and quick preparation in vitro, and easy functionalizing, aptamers were used in spectrophotometry, * fluorescence, electrochemistry, surface plasmon resonance (SPR) and surface enhanced Raman scattering (SERS) analysis [8][9][10]. RS spectral technique is simple, rapid and sensitive, and has been applied to analysis of trace proteins, nucleic acids, and inorganic ions [11][12][13]. Nanogold (NG), being of easy preparation, high electron density good biocompatibility, and RS effect, has been applied to detect protein [14,15], metal ions [16] by RS.…”

Section: Introductionmentioning

confidence: 99%

Resonance scattering spectrum detection of trace using nanogold probe as catalyst of Cu(II)-glucose reaction

Jiang

Lin

Zhang

et al. 2013

International Journal of Environmental Analytical Chemistry

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Retraction: Ultrahighly Sensitive Homogeneous Detection of DNA and MicroRNA by Using Single‐Silver‐Nanoparticle Counting

Cited by 4 publications

References 6 publications

Redox Sorption and Recovery of Silver Ions as Silver Nanocrystals on Poly(aniline‐co‐5‐sulfo‐2‐anisidine) Nanosorbents

Redox Sorption and Recovery of Silver Ions as Silver Nanocrystals on Poly(aniline‐co‐5‐sulfo‐2‐anisidine) Nanosorbents

High Specific and Ultrasensitive Isothermal Detection of MicroRNA by Padlock Probe-Based Exponential Rolling Circle Amplification

Resonance scattering spectrum detection of trace using nanogold probe as catalyst of Cu(II)-glucose reaction

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