Retrofitting of an Affordable 3D Printer: Towards a Material Efficient and Low-Cost Bioprinting System

Cortez, Juan Enrique Pérez; Sánchez, Víctor Hugo; Vázquez, Elisa; Santiago, Grissel Trujillo‐de; Álvarez, Mario Moisés; Martínez-López, J. Israel

doi:10.1016/j.procir.2022.06.028

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…The curing point of 5% GelMA hydrogel with 0.5% Irgacure 2959 was found to be ∼100 s, and ∼20 s when photocured using 5 and 30 mW/cm 2 UV (λ = 365 nm) UV intensity, respectively . Most 3D bioprinters work with an ideal UV illumination between 0 and 30 mW/cm 2 , ,− while the most common intensity is 6.9 mW/cm 2 . ,,,− Irgacure 2959 and LAP are the most common type I photoinitiators used for GelMA curing because of their water solubility, good curing efficiency, and acceptable cytotoxicity. The solubility of LAP is comparatively much higher (8.5%) compared to that of Irgacure 2959 (∼0.5%) .…”

Section: Structure Stability and Properties Of Gelma Biomaterials Inksmentioning

confidence: 99%

Gelatin Methacryloyl (GelMA)-Based Biomaterial Inks: Process Science for 3D/4D Printing and Current Status

Das,

Jegadeesan,

Basu

2024

Biomacromolecules

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Tissue engineering for injured tissue replacement and regeneration has been a subject of investigation over the last 30 years, and there has been considerable interest in using additive manufacturing to achieve these goals. Despite such efforts, many key questions remain unanswered, particularly in the area of biomaterial selection for these applications as well as quantitative understanding of the process science. The strategic utilization of biological macromolecules provides a versatile approach to meet diverse requirements in 3D printing, such as printability, buildability, and biocompatibility. These molecules play a pivotal role in both physical and chemical cross-linking processes throughout the biofabrication, contributing significantly to the overall success of the 3D printing process. Among the several bioprintable materials, gelatin methacryloyl (GelMA) has been widely utilized for diverse tissue engineering applications, with some degree of success. In this context, this review will discuss the key bioengineering approaches to identify the gelation and cross-linking strategies that are appropriate to control the rheology, printability, and buildability of biomaterial inks. This review will focus on the GelMA as the structural (scaffold) biomaterial for different tissues and as a potential carrier vehicle for the transport of living cells as well as their maintenance and viability in the physiological system. Recognizing the importance of printability toward shape fidelity and biophysical properties, a major focus in this review has been to discuss the qualitative and quantitative impact of the key factors, including microrheological, viscoelastic, gelation, shear thinning properties of biomaterial inks, and printing parameters, in particular, reference to 3D extrusion printing of GelMA-based biomaterial inks. Specifically, we emphasize the different possibilities to regulate mechanical, swelling, biodegradation, and cellular functionalities of GelMA-based bio(material) inks, by hybridization techniques, including different synthetic and natural biopolymers, inorganic nanofillers, and microcarriers. At the close, the potential possibility of the integration of experimental data sets and artificial intelligence/machine learning approaches is emphasized to predict the printability, shape fidelity, or biophysical properties of GelMA bio(material) inks for clinically relevant tissues.

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Section: Structure Stability and Properties Of Gelma Biomaterials Inksmentioning

confidence: 99%

Gelatin Methacryloyl (GelMA)-Based Biomaterial Inks: Process Science for 3D/4D Printing and Current Status

Das,

Jegadeesan,

Basu

2024

Biomacromolecules

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A study for geometrical error in micro-geometries using VAT photopolymerization

Almanza-Vázquez,

Tejeda-Alejandre,

Rodriguez

et al. 2024

Manufacturing Letters

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Bioprinting in cardiovascular medicine: possibilities, challenges, and future perspectives for low and middle-income countries

Malhi,

Padda,

Mahtani

et al. 2024

International Journal of Surgery

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Cardiovascular diseases (CVD) stemming from various factors significantly impact the quality of life (QoL) and are prevalent with high mortality rates in both developed and developing countries. In cases where pharmacotherapy proves insufficient and end-stage disease ensues, a heart transplant/surgical repair becomes the only feasible treatment option. However, challenges such as a limited supply of heart donors, complications associated with rejection, and issues related to medication compliance introduce an additional burden to healthcare services and adversely affect patient outcomes. The emergence of bioprinting has facilitated advancements in creating structures, including ventricles, valves, and blood vessels. Notably, the development of myocardial/cardiac patches through bioprinting has offered a promising avenue for revascularizing, strengthening, and regenerating ventricles. Employment loss in developing countries as a circumstance of disability or death can severely impact a family’s well-being and means for sustainable living. Innovations by means of life sustaining treatment options can provide hope for the impoverished and help reduce disability burden on the economy of low- to middle-income countries (LMICs). Such developments can have a significant impact that can last for generations, especially in developing countries. In this review, the authors delve into various types of bioprinting techniques, exploring their possibilities, challenges, and potential future applications in treating various end-stage cardiovascular conditions in LMICs.

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Retrofitting of an Affordable 3D Printer: Towards a Material Efficient and Low-Cost Bioprinting System

Cited by 3 publications

References 12 publications

Gelatin Methacryloyl (GelMA)-Based Biomaterial Inks: Process Science for 3D/4D Printing and Current Status

Gelatin Methacryloyl (GelMA)-Based Biomaterial Inks: Process Science for 3D/4D Printing and Current Status

A study for geometrical error in micro-geometries using VAT photopolymerization

Bioprinting in cardiovascular medicine: possibilities, challenges, and future perspectives for low and middle-income countries

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