Radiofrequency catheter ablation (RFCA) is a cornerstone treatment of atrial fibrillation (AF). Transfemoral venous approach with transseptal puncture to assess left atrium (LA) is a mainstream method for AF ablation. Interrupted inferior vena cava (IVC) is a rare congenital vascular anomaly which complicates electrophysiologic procedures and makes conventional approach not feasible. According to previous literature, trans-jugular and transhepatic approach were two alternative methods to achieve AF ablation. Difficulty in transseptal puncture and bleeding risk were still the major concern of these alternative approach. This report presents a 55-year-old female with an incidental finding of interrupted IVC during AF ablation. Retrograde aortic approach was applied to assess LA for RFCA according to our previous experience. Circumferential pulmonary vein isolation was achieved and confirmed by absence of pulmonary vein potentials using the ablation catheter. After the procedure, no adverse events were observed. Neither recurrence of atrial tachyarrhythmia nor valvular injury was observed during oneyear follow-up period.A 55-year-old female patient was referred to our cardiovascular clinic from primary care hospital for propafenone refractory paroxysmal AF for two years. Because of intolerable symptoms, RFCA was suggested. The hemogram, comprehensive biochemical panel, and thyroid function test were reported normal. The echocardiogram revealed normal left ventricular systolic function without structural heart disease. After careful discussion of