Retrograde dissociation of protein–surfactant complexes induced by mixed micelle formation

Jones, Malcolm N.; Gilhooly, Elaine M.; Nicholas, A.R.

doi:10.1039/ft9928802733

Cited by 15 publications

(7 citation statements)

References 15 publications

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“…Ternary aqueous solutions of PVP, SDS, and a nonionic surfactant, i.e., the pentaethylene glycol monodecyl ether (C 10 E 5 ) will also be investigated. Only scarce information is indeed available in the scientific literature about the behavior of two surfactants in the presence of one polymer in water. − To the best of our knowledge, no experimental data has been reported for the adsorption of such ternary systems on solid surfaces. C 10 E 5 does not interact with the polymer but it forms mixed micelles with SDS.…”

Section: Introductionmentioning

confidence: 99%

Adsorption of Poly(vinylpyrrolidone)/Surfactant(s) Mixtures at the Silica/Water Interface: A Calorimetric Investigation

et al. 2000

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The adsorption of binary mixtures of poly(vinylpyrrolidone) (PVP) and sodium dodecyl sulfate (SDS) and of ternary mixture of PVP, SDS, and pentaethylene glycol monodecyl ether (C10E5) onto silica has been studied by the measurements of the adsorbed amount of PVP and the heat of adsorption. The amount of PVP adsorbed from the binary mixtures decreases as the SDS concentration is increased, whereas the enthalpy change passes through a maximum at SDS concentration of ca. 4 g/L. The chain conformation of PVP adsorbed on silica has been estimated from the adsorption data in relation to the surfactant concentration. It changes from loops and tails to trains upon increasing SDS concentration. At SDS concentrations higher than 4 g/L, a comblike conformation is observed. The adsorption of PVP/SDS/C10E5 ternary mixtures depends on the polymer concentration regime.

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Section: Introductionmentioning

confidence: 99%

Adsorption of Poly(vinylpyrrolidone)/Surfactant(s) Mixtures at the Silica/Water Interface: A Calorimetric Investigation

et al. 2000

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“…It is now time to fill this gap since mixtures of at least two surfactants are frequently found in nature and in industrial applications. These mixtures of surfactants, which may have beneficial effects, are the topic of active research, the main purpose being to predict and to measure the composition of the mixed micelles which are formed (6-9) Some mixtures of two surfactants have been studied in the presence of a polyelectroly te (10)(11)(12)(13)(14). In these examples, the polymer and one surfactant are oppositely charged, whereas the second surfactant is uncharged Creeth et al (15) have studied the adsorption behavior of a mixture composed of sodium dodecyl sulfate (SDS) and hexaethy-lene glycol monododecyl ether (C 12 E 6 ) at the water/air interface in the presence of the cationic dimethyldiallylammonium chloride-acrylamide copolymer.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Interactions of Polyvinylpyrrolidone with Mixtures of Anionic and Nonionic Surfactants or Anionic and Zwitterionic Surfactants by Pulsed Field Gradient NMR

Misselyn-Bauduin

Thibaut

Grandjean

et al. 2001

Journal of Colloid and Interface Science

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The interaction of polyvinylpyrrolidone (PVP) with an anionic surfactant (sodium dodecyl sulfate, SDS), a nonionic surfactant (pentaethylene glycol monodecyl ether, C 10 E 5 ), and a zwitterionic surfactant (lauryl amido propyl betaine, LAPB) has been investigated by means of pulsed gradient spin-echo NMR (FT-PGSE NMR), allowing self-diffusion coefficients to be determined. The results confirm the strong interaction prevailing in the PVP/SDS system, whereas no association has been observed in the PVP/C 10 E 5 and PVP/LAPB systems. Mixing PVP with two surfactants, namely SDS and C 10 E 5 or SDS and LAPB, results in the formation of ternary aggregates between the polymer and the mixed micelles.

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“…Another possibility is that binding of nonionic surfactant monomers on the protein surface displaces the more harmful anionic surfactant monomers. Indeed, in protein/mixed surfactant systems, preferential binding of one surfactant species over another has been observed (34).…”

Section: Discussionmentioning

confidence: 99%

Surfactant-Induced Unfolding of Cellulase: Kinetic Studies

Stoner

Dale²,

Gualfetti³

et al. 2006

Biotechnol. Prog.

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Surfactant-induced unfolding is a significant degradation pathway for detergent enzymes. This study examines the kinetics of surfactant-induced unfolding for endoglucanase III, a detergent cellulase, under conditions of varying pH, temperature, ionic strength, surfactant type, and surfactant concentration. Interactions between protein and surfactant monomer are shown to play a key role in determining the kinetics of the unfolding process. We demonstrate that the unfolding rate can be slowed by (1) modifying protein charge and/or pH conditions to create electrostatic repulsion of ionic surfactants and (2) reducing the amount of monomeric ionic surfactant available for interaction with the enzyme (i.e., by lowering the critical micelle concentration). Additionally, our results illustrate that there is a poor correlation between thermodynamic stability in buffer (DeltaG(unfolding)) and resistance to surfactant-induced unfolding.

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Retrograde dissociation of protein–surfactant complexes induced by mixed micelle formation

Cited by 15 publications

References 15 publications

Adsorption of Poly(vinylpyrrolidone)/Surfactant(s) Mixtures at the Silica/Water Interface: A Calorimetric Investigation

Adsorption of Poly(vinylpyrrolidone)/Surfactant(s) Mixtures at the Silica/Water Interface: A Calorimetric Investigation

Investigation of the Interactions of Polyvinylpyrrolidone with Mixtures of Anionic and Nonionic Surfactants or Anionic and Zwitterionic Surfactants by Pulsed Field Gradient NMR

Surfactant-Induced Unfolding of Cellulase: Kinetic Studies

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