Retrograde fluorescent microsphere tracing of retinal and central afferents to the nucleus of the optic tract in pigmented and albino rats

Aarnoutse, Erik J.; Want, J.J.L. van der; Vrensen, Gijs F.J.M.

doi:10.1016/0304-3940(95)12159-5

Neuroscience Letters

1995

DOI: 10.1016/0304-3940(95)12159-5

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Retrograde fluorescent microsphere tracing of retinal and central afferents to the nucleus of the optic tract in pigmented and albino rats

Erik J. Aarnoutse¹,

J.J.L. van der Want

Gijs F.J.M. Vrensen

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Cited by 3 publications

(1 citation statement)

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“…[1][2][3] However, with the imaging method used here we were able for the first time to provide an in vivo synopsis of axonal transport dynamics and neuronal survival over time on a cellular level with the method of "in vivo confocal neuroimaging" (ICON). ICON was developed in our laboratory 4 and it is based on principles used in the standard repertoire of neurobiology: retrograde axonal fluorescent latex microspheres can be used to elucidate the organization of neural connectivities and circuits [5][6][7] and they can be used to label cells in tissue cultures. 8,9 Previous double-labeling studies have been used to determine the target areas of specific neuronal axons and axon collaterals.…”

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confidence: 99%

Recovery of Axonal Transport after Partial Optic Nerve Damage Is Associated with Secondary Retinal Ganglion Cell Death In Vivo

Prilloff

Henrich‐Noack

Sabel

2012

Invest. Ophthalmol. Vis. Sci.

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After damage many surviving RGCs lose their axon transport, but after approximately 3 weeks, this transport recovers again, a sign of intrinsic axon repair. Contrary to the prediction, axon transport recovery is not associated with better cell survival but rather with a second wave of cell death. Thus, the accelerated cell death associated with recovery of axon transport suggests the existence of a late retrograde cell death signal.

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mentioning

confidence: 99%

Recovery of Axonal Transport after Partial Optic Nerve Damage Is Associated with Secondary Retinal Ganglion Cell Death In Vivo

Prilloff

Henrich‐Noack

Sabel

2012

Invest. Ophthalmol. Vis. Sci.

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Retinorecipient areas in the diurnal murine rodent Arvicanthis niloticus: A disproportionally large superior colliculus

Gaillard

Karten

Sauvé

2013

J of Comparative Neurology

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The Nile grass rat (Arvicanthis niloticus) has a high proportion of cone photoreceptors (∼30-40%) compared with that in the common laboratory mouse and rat (∼1-3%) and may prove a preferable murine model with which to study cone-driven information processing in retina and primary visual centers. However, other than regions involved in circadian control, little is known about the retinorecipient structures in this rodent. We undertook a detailed analysis of the retinal projections as revealed after intravitreal injection of the anterograde tracer cholera toxin subunit B. Retinal efferents were evaluated in 45 subcortical structures. Contralateral projections were always dominant. Major contralateral inputs consisted of the suprachiasmatic nucleus, dorsolateral geniculate nucleus (dLGN), intergeniculate leaflet, ventral geniculate nucleus (magnocellular part), lateroposterior thalamic nucleus, all six pretectal nuclei, superficial layers of the superior colliculus (SC), and the main nuclei of the accessory optic system. Terminals from the contralateral eye were also localized in an unnamed field rostromedial to the dLGN as well as in the subgeniculate thalamic nucleus. Ipsilateral inputs were found mainly in the suprachiasmatic nucleus, dLGN, intergeniculate leaflet, internal sector of the magnocellular part of the ventral geniculate nucleus, olivary pretectal nucleus, and SC optic layer. Retinal afferents were not detected in the basal forebrain or the dorsal raphe nucleus. Morphometric measurements revealed that the superficial layers of the SC are disproportionately enlarged relative to other retinorecipient regions and brain size compared with rats and mice. We suggest that this reflects the selective projection of cone-driven retinal ganglion cells to the SC.

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In vivo confocal neuroimaging (ICON): non‐invasive, functional imaging of the mammalian CNS with cellular resolution

Prilloff

Fan

Henrich‐Noack

et al. 2010

Eur J of Neuroscience

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With in vivo confocal neuroimaging (ICON), single retinal ganglion cells (RGCs) can be visualized non-invasively, repeatedly, in real-time and under natural conditions. Here we report the use of ICON to visualize dynamic changes in RGC morphology, connectivity and functional activation using calcium markers, and to visualize nanoparticle transport across the blood-retina barrier by fluorescent dyes. To document the versatility of ICON, we studied the cellular response to optic nerve injury, and found evidence of reversible soma swelling, recovery of retrograde axonal transport and a difference in calcium activation dynamics between surviving and dying RGCs. This establishes ICON as a unique tool for studying CNS physiology and pathophysiology in real-time on a cellular level. ICON has potential applications in different research fields, such as neuroprotection/regeneration, degeneration, pharmacology, toxicity and drug delivery.

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Retrograde fluorescent microsphere tracing of retinal and central afferents to the nucleus of the optic tract in pigmented and albino rats

Cited by 3 publications

References 25 publications

Recovery of Axonal Transport after Partial Optic Nerve Damage Is Associated with Secondary Retinal Ganglion Cell Death In Vivo

Recovery of Axonal Transport after Partial Optic Nerve Damage Is Associated with Secondary Retinal Ganglion Cell Death In Vivo

Retinorecipient areas in the diurnal murine rodent Arvicanthis niloticus: A disproportionally large superior colliculus

In vivo confocal neuroimaging (ICON): non‐invasive, functional imaging of the mammalian CNS with cellular resolution

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