Retrograde transneuronal transfer of Herpes simplex virus type 1 (HSV 1) from motoneurones

Ugolini, Gabriella; Kuypers, H.G.J.M.; Simmons, Anthony

doi:10.1016/0006-8993(87)90931-0

Cited by 192 publications

(131 citation statements)

References 40 publications

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“…At this dose, ␤-gal-positive cells were identified in a variety of loci within the CNS: the injection site (striatum), and distal sites in the prefrontal cortex, some thalamic nuclei and the substantia nigra, in agreement with retrograde transport of HSV. 60,61 The distribution pattern reported here is very similar to the one described by McMenamim and co-workers. 62 As expected, expression from the HCMV IE promoter was down-regulated efficiently shortly after delivery, and by day 14 after surgery no transduced cells were detected.…”

Section: Discussionsupporting

confidence: 89%

Latency associated promoter transgene expression in the central nervous system after stereotaxic delivery of replication-defective HSV-1-based vectors

et al. 2001

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The herpes simplex virus type 1 (HSV-1) latency associated promoter (LAP) has been shown to sustain long-term reporter gene expression within sensory neurones. Its activity within the CNS is, however, less well understood. In this study we characterise the activity of the LAP after stereotaxic delivery of recombinant HSV-1-based vectors to the brain. Two classes of vectors were utilised in these studies: (1) a replication-defective vector lacking the glycoprotein H and thymidine kinase genes, designated CS1, and (2) a virus mutant severely impaired for immediate-early (IE) gene expression which lacks functional VP16, ICP4 and ICP0 genes, designated in1388. Both vectors contain the LacZ gene under the control of the LAP. Following delivery of either vector to the striatum, ␤-gal expression was detected within anatomically related CNS regions distal to the site of injection. At these sites the number of ␤-gal-positive cells increased with time and remained stable up to 4 weeks p.i. ␤-Gal expression could not be detected at the site

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Section: Discussionsupporting

confidence: 89%

Latency associated promoter transgene expression in the central nervous system after stereotaxic delivery of replication-defective HSV-1-based vectors

et al. 2001

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“…[28][29][30] Therefore, it may be reasonable that the transport and spread in the spinal cord may be via the axon from the nerve ending in the muscle to the motor neurons and then transneuronal spread from anterior horn motor neurons to the other side of neuronal cells in the spinal cord. 31 Therefore, ␤H1 may have traveled from the inoculation site to the anterior horn motor neurons without local replication or transneuronal spread to other neurons in the wide range of the spinal cord.…”

Section: Discussionmentioning

confidence: 99%

Long-term gene expression in the anterior horn motor neurons after intramuscular inoculation of a live herpes simplex virus vector

et al. 2000

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To clarify the feasibility of the herpes simplex virus (HSV) vector in expressing the foreign gene in the motor neuron, we inoculated a live attenuated HSV expressing ␤-galactosidase (␤-gal) activity under a latency-associated transcript promoter in the right gastrocnemius muscle of rats. Expression of ␤-gal activity was observed 5 days after inoculation in the bilateral anterior horn cells of the spinal cord that innervates the inoculation muscle. However, the spread of ␤-gal activity was not observed in the inoculation muscle. Without significant pathological changes, the spread of ␤-gal-expressing neurons was observed in the lumbosacral

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“…Neurotropic viruses, specifically herpesviruses, possess an advantage to these other contemporary methods in that they are able to replicate within neuron cell bodies providing signal amplification before infecting secondand third-order neurons. The herpesviruses have also been shown to specifically label neuronal connections in both the retrograde and anterograde direction (Ugolini et al, 1987;Norgren Jr, et al, 1992).…”

Section: A Tool For Transneuronal Tracing Studiesmentioning

confidence: 99%

The roles of herpes simplex virus in neuroscience

Turner¹,

Jenkins²

1997

J Neurovirol

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Herpes simplex virus (HSV) has been a focus of research in many laboratories during the last 30 ± 35 years, with the majority centered on the virus' replication, molecular biology and pathogenesis. Recently, HSV has begun to receive considerable attention in the field of neuroscience, where scientists have begun to use the virus as a tool or model for several areas of investigation. These areas include the construction and development of HSV-based vectors for gene therapy and the use of HSV as a neuronal tracer, as a model for demyelinating disease and to study interactions between the nervous, immune and endocrine systems. The goal of this paper is to review these different roles for HSV in the broad field of neuroscience.

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Retrograde transneuronal transfer of Herpes simplex virus type 1 (HSV 1) from motoneurones

Cited by 192 publications

References 40 publications

Latency associated promoter transgene expression in the central nervous system after stereotaxic delivery of replication-defective HSV-1-based vectors

Latency associated promoter transgene expression in the central nervous system after stereotaxic delivery of replication-defective HSV-1-based vectors

Long-term gene expression in the anterior horn motor neurons after intramuscular inoculation of a live herpes simplex virus vector

The roles of herpes simplex virus in neuroscience

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