Retrospective Analysis of the Effects of Identification Procedures and Cage Changing by Using Data from Automated, Continuous Monitoring

Lim, Manho; Defensor, Erwin B.; Mechanic, Jordan; Shah, Puja P; Jaime, Evelyn A; Roberts, Clifford R.; Hutto, David; Schaevitz, Laura

doi:10.30802/aalas-jaalas-18-000056

Cited by 25 publications

(19 citation statements)

References 19 publications

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“…As described elsewhere ( Lim et al, 2017 , 2019 ; Do et al accepted in Comparative Medicine), Vium Digital Smart Houses consist of standard IVC slotted in Vium’s rack system. The Digital Smart Houses are outfitted with sensors and high-definition (HD) cameras that enable continuous, 24/7 monitoring of animals that streams data to a secure cloud-based infrastructure.…”

Section: Methodsmentioning

confidence: 99%

“…The Digital Smart Houses are outfitted with sensors and high-definition (HD) cameras that enable continuous, 24/7 monitoring of animals that streams data to a secure cloud-based infrastructure. Video is processed using computer vision algorithms to produce a digital history of body motion (mm/s) and breathing rate (breaths per min or bpm; Lim et al, 2017 , 2019 ; Do et al accepted in Comparative Medicine). Briefly, to compute body motion, computer vision algorithms use visible and infrared spectrum video captured by an HD camera at 24 frames per second.…”

Section: Methodsmentioning

confidence: 99%

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Continuous, Automated Breathing Rate and Body Motion Monitoring of Rats With Paraquat-Induced Progressive Lung Injury

Baran¹,

Gupta

Lim³

et al. 2020

Front. Physiol.

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Assessments of respiratory response and animal activity are useful endpoints in drug pharmacology and safety research. We investigated whether continuous, direct monitoring of breathing rate and body motion in animals in the home cage using the Vum Digital Smart House can complement standard measurements in enabling more granular detection of the onset and severity of physiologic events related to lung injury in a wellestablished rodent model of paraquat (PQ) toxicity. In rats administered PQ, breathing rate was significantly elevated while body motion was significantly reduced following dosing and extending throughout the 14-day study duration for breathing rate and at least 5 days for both nighttime and daytime body motion. Time course differences in these endpoints in response to the potential ameliorative test article bardoxolone were also readily detected. More complete than standard in-life measurements, breathing rate and body motion tracked injury progression continuously over the full study time period and aligned with, and informed on interval changes in clinical pathology. In addition, breathing rates correlated with terminal pathology measurements, such as normalized lung weights and histologic alveolar damage and edema. This study is a preliminary evaluation of the technology; our results demonstrate that continuously measured breathing rate and body motion served as physiologically relevant readouts to assess lung injury progression and drug response in a respiratory injury animal model.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Continuous, Automated Breathing Rate and Body Motion Monitoring of Rats With Paraquat-Induced Progressive Lung Injury

Baran¹,

Gupta

Lim³

et al. 2020

Front. Physiol.

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“…This can be achieved through telemetry, and some physiologic parameters, such as respiratory and heart rates, can even be measured by automated 'smart caging'. 44,77 Assessment of these physiologic variables should never be used alone when attempting to assess pain in mice and rats.…”

Section: Physiologic Assessments Of Pain In Laboratory Mice and Ratsmentioning

confidence: 99%

A Review of Pain Assessment Methods in Laboratory Rodents

2019

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Ensuring that laboratory rodent pain is well managed underpins the ethical acceptability of working with these animals in research. Appropriate treatment of pain in laboratory rodents requires accurate assessments of the presence or absence of pain to the extent possible. This can be challenging some situations because laboratory rodents are prey species that may show subtle signs of pain. Although a number of standard algesiometry assays have been used to assess evoked pain responses in rodents for many decades, these methods likely represent an oversimplification of pain assessment and many require animal handling during testing, which can result in stress-induced analgesia. More recent pain assessment methods, such as the use of ethograms, facial grimace scoring, burrowing, and nest-building, focus on evaluating changes in spontaneous behaviors or activities of rodents in their home environments. Many of these assessment methods are time-consuming to conduct. While many of these newer tests show promise for providing a more accurate assessment of pain, most require more study to determine their reliability and sensitivity across a broad range of experimental conditions, as well as between species and strains of animals. Regular observation of laboratory rodents before and after painful procedures with consistent use of 2 or more assessment methods is likely to improve pain detection and lead to improved treatment and care—a primary goal for improving overall animal welfare.

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“…Vium Digital Smart Houses consist of standard individually ventilated cage slotted in Vium's proprietary rack system. The Digital Vivarium test was described previously [35]. Briefly, Vium Digital Smart Houses are outfitted with sensors and a high-definition camera that enable continuous monitoring of animals.…”

Section: Digital Vivariummentioning

confidence: 99%

Behavioral and electrophysiological evidence for a neuroprotective role of aquaporin-4 in the 5xFAD transgenic mice model

Abe

Ikegawa

Yoshida

et al. 2020

acta neuropathol commun

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Aquaporin-4 (AQP4) has been suggested to be involved in the pathogenesis of neurodegenerative diseases including Alzheimer's disease (AD), which may be due to the modulation of neuroinflammation or the impairment of interstitial fluid bulk flow system in the central nervous system. Here, we show an age-dependent impairment of several behavioral outcomes in 5xFAD AQP4 null mice. Twenty-four-hour video recordings and computational analyses of their movement revealed that the nighttime motion of AQP4-deficient 5xFAD mice was progressively reduced between 20 and 36 weeks of age, with a sharp deterioration occurring between 30 and 32 weeks. This reduction in nighttime motion was accompanied by motor dysfunction and epileptiform neuronal activities, demonstrated by increased abnormal spikes by electroencephalography. In addition, all AQP4-deficient 5xFAD mice exhibited convulsions at least once during the period of the analysis. Interestingly, despite such obvious phenotypes, parenchymal amyloid β (Aβ) deposition, reactive astrocytosis, and activated microgliosis surrounding amyloid plaques were unchanged in the AQP4-deficient 5xFAD mice relative to 5xFAD mice. Taken together, our data indicate that AQP4 deficiency greatly accelerates an age-dependent deterioration of neuronal function in 5xFAD mice associated with epileptiform neuronal activity without significantly altering Aβ deposition or neuroinflammation in this mouse model. We therefore propose that there exists another pathophysiological phase in AD which follows amyloid plaque deposition and neuroinflammation and is sensitive to AQP4 deficiency.

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Retrospective Analysis of the Effects of Identification Procedures and Cage Changing by Using Data from Automated, Continuous Monitoring

Cited by 25 publications

References 19 publications

Continuous, Automated Breathing Rate and Body Motion Monitoring of Rats With Paraquat-Induced Progressive Lung Injury

Continuous, Automated Breathing Rate and Body Motion Monitoring of Rats With Paraquat-Induced Progressive Lung Injury

A Review of Pain Assessment Methods in Laboratory Rodents

Behavioral and electrophysiological evidence for a neuroprotective role of aquaporin-4 in the 5xFAD transgenic mice model

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