Retrospective analysis of the key molecules involved in the green synthesis of nanoparticles

Khan, Fazlurrahman; Jeong, Geum-Jae; Singh, Priyanka; Tabassum, Nazia; Mijaković, Ivan; Kim, Young‐Mog

doi:10.1039/d2nr03632k

Cited by 28 publications

(20 citation statements)

References 157 publications

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“…Emerging nanotechnology, notably the use of nanoparticles in managing a wide spectrum of bacterial pathogens, has emerged as a promising strategy . Nanoparticles’ features, such as small size, large surface area, biocompatibility, and targeting to specific sites, make them more suited for use in various biochemical functions. , Most studies used biologically derived raw materials (from algae, fungi, bacteria, and mammals) to synthesize organic or inorganic nanoparticles to treat infectious and noninfectious diseases due to their various benefits over chemically synthesized nanoparticles. − We synthesize two different kinds of metallic nanoparticles, such as silver (AgNPs) and gold (AuNPs), using microbial-derived products as raw materials, which have several advantages. According to a recent study, probiotics, particularly lactic acid bacteria (LAB), have drawn much interest from the food and pharmaceutical industries because of their many advantages for human health and well-being. − Several studies have shown that LAB provides biological protection against microbial infections. , As a result, it would be one of the novel approaches to using LAB as an environmentally friendly method of synthesizing biocompatible NPs that can be used to treat a wide variety of microbial infections. , Thus, in the current study, we sought to isolate and characterize the LAB strains from the Kimchi sample as a biological source for the synthesis of AuNPs and AgNPs.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Biofilm and Virulence Properties of Pathogenic Bacteria by Silver and Gold Nanoparticles Synthesized from Lactiplantibacillus sp. Strain C1

et al. 2023

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The emergence of antibiotic resistance in microbial pathogens necessitates the development of alternative ways to combat the infections that arise. The current study used nanotechnology as an alternate technique to control virulence characteristics and biofilm development in Pseudomonas aeruginosa and Staphylococcus aureus. Furthermore, based on the acceptance and biocompatibility of the probiotic bacteria, we chose a lactic acid bacteria (LAB) for synthesizing two types of metallic nanoparticles (NPs) in this study. Using molecular techniques, the LAB strain C1 was isolated from Kimchi food samples and identified as Lactiplantibacillus sp. strain C1. The prepared supernatant from strain C1 was used to produce gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs). C1-AuNPs and C1-AgNPs were characterized physiochemically using a variety of instruments. C1-AuNPs and C1-AgNPs had spherical shapes and sizes of 100.54 ± 14.07 nm (AuNPs) and 129.51 ± 12.31 nm (AgNPs), respectively. C1-AuNPs and C1-AgNPs were discovered to have high zeta potentials of −23.29 ± 1.17 and −30.57 ± 0.29 mV, respectively. These nanoparticles have antibacterial properties against several bacterial pathogens. C1-AuNPs and C1-AgNPs significantly inhibited the initial stage biofilm formation and effectively eradicated established mature biofilms of P. aeruginosa and S. aureus. Furthermore, when P. aeruginosa was treated with sub-MIC levels of C1-AuNPs and C1-AgNPs, their different virulence features were significantly reduced. Both NPs greatly inhibited the hemolytic activity of S. aureus. The inhibition of P. aeruginosa and S. aureus biofilms and virulence features by C1-AuNPs and C1-AgNPs can be regarded as viable therapeutic strategies for preventing infections caused by these bacteria.

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Section: Introductionmentioning

confidence: 99%

Inhibition of Biofilm and Virulence Properties of Pathogenic Bacteria by Silver and Gold Nanoparticles Synthesized from Lactiplantibacillus sp. Strain C1

et al. 2023

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“…Furthermore, the green technique for nanoparticle synthesis offers significant benefits over the chemical-based technique [ 13 ]. Green synthesis NPs have several benefits over chemical and physical approaches, including being easier to synthesize, eco-friendly, cost-effective, minimizing the release of harmful compounds into the environment, and producing stable and biocompatible NPs [ 14 , 15 ]. There are more and more reports on the production of nanoparticles from biologically derived materials such as plants, algae, fungi, bacteria, and animals [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm and Antivirulence Activities of Gold and Zinc Oxide Nanoparticles Synthesized from Kimchi-Isolated Leuconostoc sp. Strain C2

Kang

Khan

et al. 2022

Antibiotics

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The rapid emergence of antimicrobial resistance (AMR) among bacterial pathogens results in antimicrobial treatment failure and the high mortality rate associated with AMR. The application of nanoparticles synthesized from probiotics will be widely accepted due to their efficacy and biocompatibility in treating microbial infections in humans. The current work sought to isolate and identify lactic acid bacteria (LAB) from Kimchi. Based on 16S rRNA gene sequencing, the LAB isolate C2 was identified as a member of the genus Leuconostoc. The obtained supernatant from Leuconostoc sp. strain C2 was employed for the green synthesis of metal (AuNPs) and metal oxide (ZnONPs) nanoparticles. UV–vis absorption spectra, FTIR analysis, XRD, DLS, FE-TEM, and EDS mapping were used to fully characterize these C2-AuNPs and C2-ZnONPs. The C2-AuNPs were found to be spherical in shape, with a size of 47.77 ± 5.7 nm and zeta potential of −19.35 ± 0.67 mV. The C2-ZnONPs were observed to be rod-shaped and 173.77 ± 14.53 nm in size. The C2-ZnONPs zeta potential was determined to be 26.62 ± 0.35 mV. The C2-AuNPs and C2-ZnONPs were shown to have antimicrobial activity against different pathogens. Furthermore, these nanoparticles inhibited the growth of Candida albicans. The antibiofilm and antivirulence properties of these NPs against Pseudomonas aeruginosa and Staphylococcus aureus were thoroughly investigated. C2-AuNPs were reported to be antibiofilm and antivirulence against P. aeruginosa, whereas C2-ZnONPs were antibiofilm and antivirulence against both P. aeruginosa and S. aureus. Furthermore, these nanoparticles disrupted the preformed mature biofilm of P. aeruginosa and S. aureus. The inhibitory impact was discovered to be concentration-dependent. The current research demonstrated that C2-AuNPs and C2-ZnONPs exhibited potential inhibitory effects on the biofilm and virulence features of bacterial pathogens. Further studies are needed to unravel the molecular mechanism behind biofilm inhibition and virulence attenuation.

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“…Nanoparticles, particularly metallic ones, have been widely used in the treatment of microbial pathogens due to several advantages, including specific targeting, an effective drug delivery system, less cytotoxicity, and controlled release [ 22 , 23 , 24 ]. A growing number of research studies has focused on using naturally occurring substances from plants, algae, fungi, bacteria, or animals in the synthesis of metallic nanoparticles [ 25 , 26 ]. The use of marine-derived materials in the synthesis of nanoparticles has received particular attention due to their ease of availability, low cost in the isolation of active molecules, biocompatibility, biodegradability, and potential bioactive properties [ 27 , 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Polymicrobial Biofilms of Candida albicans–Staphylococcus aureus/Streptococcus mutans by Fucoidan–Gold Nanoparticles

et al. 2023

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The polymicrobial proliferation and development of complex biofilm morphologies by bacterial and fungal pathogens in the host are some of the key factors contributing to the failure of antimicrobial treatments. The polymicrobial interaction of Candida albicans and some bacterial species has been extensively studied in both in vitro and in vivo model systems. Alternative strategies for disrupting polymicrobial interaction and biofilm formation are constantly needed. Among several alternative strategies, the use of nanoparticles synthesized using a natural product in the treatment of microbial infection has been considered a promising approach. The current study aimed to synthesize gold nanoparticles (AuNPs) using a natural product, fucoidan, and to test their efficacy against mono and duo combinations of fungal (Candida albicans) and bacterial (Staphylococcus aureus/Streptococcus mutans) biofilms. Several methods were used to characterize and study Fu–AuNPs, including UV-vis absorption spectroscopy, FTIR, FE-TEM, EDS, DLS, zeta potential, and XRD. The concentration-dependent inhibition of early-stage biofilms and the eradication of mature biofilms of single species of C. albicans, S. aureus, and S. mutans have been observed. Early biofilms of a dual-species combination of C. albicans and S. aureus/S. mutans were also suppressed at an increasing concentration of Fu–AuNPs. Furthermore, Fu–AuNPs significantly eradicated the established mature biofilm of mixed species. The treatment method proposed in this study, which involves the use of marine-bioinspired nanoparticles, is a promising and biocompatible agent for preventing the growth of polymicrobial biofilms of bacterial and fungal pathogens.

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Retrospective analysis of the key molecules involved in the green synthesis of nanoparticles

Abstract: Emerging nanotechnology offers great interest and success in synthesizing and applying nanoparticles (NPs) using the green-chemistry approach. Since NPs synthesized using naturally derived materials are a potential alternative compared to...

Cited by 28 publications

References 157 publications

Inhibition of Biofilm and Virulence Properties of Pathogenic Bacteria by Silver and Gold Nanoparticles Synthesized from Lactiplantibacillus sp. Strain C1

Inhibition of Biofilm and Virulence Properties of Pathogenic Bacteria by Silver and Gold Nanoparticles Synthesized from Lactiplantibacillus sp. Strain C1

Antibiofilm and Antivirulence Activities of Gold and Zinc Oxide Nanoparticles Synthesized from Kimchi-Isolated Leuconostoc sp. Strain C2

Inhibition of Polymicrobial Biofilms of Candida albicans–Staphylococcus aureus/Streptococcus mutans by Fucoidan–Gold Nanoparticles

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