Retrospective study of prognostic importance of DNA flow cytometry of urinary bladder carcinoma.

Blomjous, C. E. M.; Schipper, N. W.; Baak, Jan P. A.; Galen, Edward; Voogt, H. J. de; Meyer, C. J. L. M.

doi:10.1136/jcp.41.1.21

Cited by 67 publications

(24 citation statements)

References 23 publications

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“…In patients with superficial bladder cancer (Ta, TI, TIS), DNA cytometry provides prognostic information for stage progression to muscle invasion and metastases. DNA ploidy status listed in order of decreasing risk for progression is: multiple DNA aneuploid populations > single DNA aneuploid population > DNA "tetraploid" population > DNA diploid population (7,8,11,14,16,(18)(19)(20)31,33). The clinical value of DNA analysis in T2-T4 bladder cancer remains to be established and does not provide, at this time, a basis for treatment recommendations (6,25,33,40,4 …”

Section: Tumor (Ta T Tis) Eventmentioning

confidence: 99%

“…DNA cytometry should be used only in patients with tumor, a past history of tumor, or in patients in which there is a strong suspicion of tumor. DNA ploidy status of irrigation and tumor specimens correlates with grade and stage (2, 7,8,16,35). The maximum utility of DNA cytometry is in stratifying grade 2 superficial (Ta, TI, TIS) tumors (16,30,36).…”

Section: Clinical Utility Of Dna Cytometrymentioning

confidence: 99%

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Consensus review of the clinical utility of dna cytometry in bladder cancer

et al. 1993

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Section: Tumor (Ta T Tis) Eventmentioning

confidence: 99%

Section: Clinical Utility Of Dna Cytometrymentioning

confidence: 99%

Consensus review of the clinical utility of dna cytometry in bladder cancer

et al. 1993

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“…In bladder cancer the presence of aneuploid cell populations usually correlates with tumour aggression, the most invasive tumours often containing a triploid DNA component (Blomjous et al, 1988;Tribukait, 1984). The original tumour from which UCRU BL 17CL was derived was aggressive in its behaviour, resulting in the death of the patient only four months after presentation (Russell et al, 1988a).…”

Section: Growth In Vivomentioning

confidence: 99%

Clonal analysis of a bladder cancer cell line: an experimental model of tumour heterogeneity

Brown

Russell²,

Philips³

et al. 1990

Br J Cancer

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Summary The continuous cell line UCRU BL 17CL was derived from a human invasive bladder cancer and expresses elements of transitional, squamous and glandular differentiation. Nine clones of this line were established by limit dilution and have been extensively characterised. Only six of these clones grew subcutaneously in nude mice. Of these, three have exhibited local invasion, each in one of five implanted mice. Although all xenografts expressed transitional, squamous and glandular elements, different histological subtypes predominated within each clone. Only clones which grew in nude mice formed colonies in semi-solid medium, and each responded differently to the influence of medium that had been conditioned by the growth of UCRU BL 17CL, suggesting the possible secretion of a growth factor by these cells. The DNA content and lectin binding profiles of the clones also reflected the heterogeneity of the line. UCRU BL 17CL and the nine clones provide a unique model for the study of tumour heterogeneity, progression and differentiation, and the potential autocrine regulation of growth of bladder cancer.In human bladder cancer, and in many other cancer types,

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“…These variables may indicate the state of progression and the proliferative potential of a tumor, and previous reports have shown a prognostic value in a number of neoplasms (17,123). In bladder cancer several authors have found a correlation between aneuploidy and T-classification and between aneuploidy and histological grade (4,6,13,16,28). A correlation between S-phase size and heterogeneity versus T-classification and grade has been demonstrated ( 13).…”

mentioning

confidence: 99%

Prognostic significance of dna content in bladder cancer based on flow cytometric analysis of 249 transitional cell carcinomas

Vindeløv

Christensen²,

Engelholm

et al. 1995

Cytometry

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The prognostic significance of DNA index (DI), S-phase fraction, and heterogeneity determined by flow cytometric DNA analysis was assessed in a prospective study of 249 newly diagnosed transitional cell carcinomas of the bladder. The median observation time was 4.8 years. A total of 456 subpopulations were detected. The S-phases could be estimated in 299 subpopulations. A DI > 1.25 or an S-phase above 9.7%were strongly correlated to invasiveness.One hundred and ten patients were treated with transurethral resection (TUR). Relapse-free survival could not be predicted by the DNA-derived parameters. Univariate analysis of survival showed prognostic significance of diploidy (0.98 < DI 5 1.02, P = 0.021, hypotetraploidy (1.50 < DI 5 1.96, P = 0.0021, and S-phase size (P = 0.008). Multivariate analysis pointed to the T-classification (RR = 1.64) and hypotetraploidy (RR = 1.57) as prognostic parameters for survival of TUR-treated patients.One hundred and thirty-nine patients received radiotherapy (RT). A significantly better response was found for tumors with a subpopulation with a hypertetraploid DNA content (DI > 2.04, P = 0.051, and a significantly worse response for subpopulations with a maximum S-phase > 24.5% ( P = 0.04). T-classification and histological grade had no predictive value. A logistic regression analysis indicated an estimated probability of response to RT of 77% for tumors with a DI > 2.04 and an S-phase < 24.5%, whereas tumors with a DI < 2.04 and an S-phase > 24.5% had only a 28% probability of response. The poor response to RT, predicted by an S-phase > 24.5%, translated into a poor survival, whereas the better treatment response found for patients with a DI > 2.04 did not result in a longer survival. Multivariate analysis pointed to S-phase (RR = 1.701, T-classification (RR = 1.601, and grade (RR = 0.65) as independent prognostic parameters for survival of RT-treated patients. o 1995 Wiley-Liss, Inc.Key terms: Detergent-trypsin preparation, propidium iodide, chicken and trout erythrocyte standardization, multivariate analysis of survival, treatment response Transitional cell carcinoma of the bladder is a disease entity encompassing a wide spectrum of tumors ranging from relatively benign non-invasive neoplasms with a low capacity for progression to rapidly disseminating cancers with a high mortality rate. The best prognostic parameters in general use today are the T-classification and histological grade (9,19,22). These parameters are influenced by the subjectivity of the urologist and the pathologist ( 20) and therefore not completely reproducible. Furthermore considerable variation in the clinical course may be found for tumors of identical class and grade. There is thus a need for additional and preferentially objective measurements with prognostic significance in bladder cancer.The prognostic value of tumor DNA content has been recognised for a number of years (2). Flow cytometric DNA analysis yields information on stem-line DNA abnormality, heterogeneity, and S-phase size. These variables

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Retrospective study of prognostic importance of DNA flow cytometry of urinary bladder carcinoma.

Cited by 67 publications

References 23 publications

Consensus review of the clinical utility of dna cytometry in bladder cancer

Consensus review of the clinical utility of dna cytometry in bladder cancer

Clonal analysis of a bladder cancer cell line: an experimental model of tumour heterogeneity

Prognostic significance of dna content in bladder cancer based on flow cytometric analysis of 249 transitional cell carcinomas

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