Retroviral and Lentiviral Vectors for the Induction of Immunological Tolerance

Dufait, Inès; Liechtenstein, Therese; Lanna, Alessio; Bricogne, Christopher; Laranga, Roberta; Padella, Antonella; Breckpot, Karine; Escors, David

doi:10.6064/2012/694137

Cited by 41 publications

(34 citation statements)

References 162 publications

(212 reference statements)

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“…Lentiviruses are one of nature's most efficient gene delivery vehicles, having been used for gene therapy of inherited disorders(12, 13) and immune gene therapy for cancer (14, 15) and autoimmune disease (16) and in the generation of transgenic animals through microinjection into the perivitelline space of oocytes or fertilized eggs of mice (17), rats (18), rabbits (19), nonhuman primates (20), and chickens (21) or quail embryos (22). Here, we hypothesize that lentivirally transduced spermatozoa should be capable of acting as vehicles to deliver a transgene [green fluorescent protein (GFP)] to ovulated eggs for the generation of transgenic mice.…”

mentioning

confidence: 99%

Efficient generation of transgenic mice by lentivirus‐mediated modification of spermatozoa

Chandrashekran¹,

Sarkar²,

Thrasher

et al. 2013

FASEB j.

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Transgenic technologies conventionally rely on the oocyte as a substrate for genetic modification. Owing to their accessibility, however, male germ cells, including mature sperm, have material advantages for use in transgenesis. Here we have exploited lentiviruses to generate transgenic animals via the male germline. When pseudotyped lentiviral vectors encoding green fluorescent protein (GFP) were incubated with mouse spermatozoa, these sperm were highly successful in producing transgenics. Lentivirally transduced mouse spermatozoa were used in in vitro fertilization (IVF) studies, and when followed by embryo transfer, ≥ 42% of founders were found to be transgenic for GFP. Inverse PCR strategy for integration site analysis demonstrated integration of at least 1 or 2 copies of GFP in the transgenics, mapping to different chromosomes. GFP expression was detected in a wide range of murine tissues, including testis and the transgene was stably transmitted to a third generation of transgenic animals. This relatively simple, yet highly efficient, technique for generating transgenic animals by transducing spermatozoa with lentiviral vectors in vitro is a powerful tool for the study of fertilization/preimplantation development, vertical viral gene transmission, gene function and regulation, and epigenetic inheritance.

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mentioning

confidence: 99%

Efficient generation of transgenic mice by lentivirus‐mediated modification of spermatozoa

Chandrashekran¹,

Sarkar²,

Thrasher

et al. 2013

FASEB j.

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“…Gene silencing of different pro‐inflammatory molecules, such as CD40, CD80, CD86 and IL‐12 in DCs promotes a tolerogenic phenotype that may improve autoimmune diseases, mainly by suppressing the activation of T and B cells and expanding Treg cell subsets . These data underscore the potential use of DC manipulation with lentivirus transduction expressing interference RNA for co‐stimulatory molecules, to induce a tolerogenic phenotype …”

Section: Targeting DC Function As a Therapeutic Approach For Autoimmumentioning

confidence: 93%

“…[159][160][161] These data underscore the potential use of DC manipulation with lentivirus transduction expressing interference RNA for co-stimulatory molecules, to induce a tolerogenic phenotype. 162,163 Although there are several studies reporting the generation of tolDCs from patients with multiple sclerosis, which induce hyporesponsiveness in myelin-specific autologous T cells, to date there are no clinical trials with tolDC therapy in multiple sclerosis. [164][165][166] Nevertheless, this strategy is a promising cell therapy for the treatment of immune-mediated diseases.…”

Section: Interaction With B Cells and Treg Cells During Sle Pathogmentioning

confidence: 99%

Contribution of dendritic cells to the autoimmune pathology of systemic lupus erythematosus

et al. 2015

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SummarySystemic lupus erythematosus (SLE) is a heterogeneous disease in which excessive inflammation, autoantibodies and complement activation lead to multisystem tissue damage. The contribution of the individual genetic composition has been extensively studied, and several susceptibility genes related to immune pathways that participate in SLE pathogenesis have been identified. It has been proposed that SLE takes place when susceptibility factors interact with environmental stimuli leading to a deregulated immune response. Experimental evidence suggests that such events are related to the failure of T-cell and B-cell suppression mediated by defects in cell signalling, immune tolerance and apoptotic mechanism promoting autoimmunity. In addition, it has been reported that dendritic cells (DCs) from SLE patients, which are crucial in the modulation of peripheral tolerance to self-antigens, show an increased ratio of activating/inhibitory receptors on their surfaces. This phenotype and an augmented expression of co-stimulatory molecules is thought to be critical for disease pathogenesis. Accordingly, tolerogenic DCs can be a potential strategy for developing antigen-specific therapies to reduce detrimental inflammation without causing systemic immunosuppression. In this review article we discuss the most relevant data relative to the contribution of DCs to the triggering of SLE.

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“…However, the drawback of retroviruses is their inability to infect quiescent cells [249]. In contrast, lentiviruses can transduce non-dividing cells [250][251][252][253], which makes these viruses popular for use in the transfection of cells for tissue engineering.…”

Section: Viral Transductionmentioning

confidence: 99%

Noncoding RNAs and Their Potential Therapeutic Applications in Tissue Engineering

Qian

Wang

et al. 2017

Engineering

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Tissue engineering is a relatively new but rapidly developing field in the medical sciences. Noncoding RNAs (ncRNAs) are functional RNA molecules without a protein-coding function; they can regulate cellular behavior and change the biological milieu of the tissue. The application of ncRNAs in tissue engineering is starting to attract increasing attention as a means of resolving a large number of unmet healthcare needs, although ncRNA-based approaches have not yet entered clinical practice. In-depth research on the regulation and delivery of ncRNAs may improve their application in tissue engineering. The aim of this review is: to outline essential ncRNAs that are related to tissue engineering for the repair and regeneration of nerve, skin, liver, vascular system, and muscle tissue; to discuss their regulation and delivery; and to anticipate their potential therapeutic applications.

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Retroviral and Lentiviral Vectors for the Induction of Immunological Tolerance

Cited by 41 publications

References 162 publications

Efficient generation of transgenic mice by lentivirus‐mediated modification of spermatozoa

Efficient generation of transgenic mice by lentivirus‐mediated modification of spermatozoa

Contribution of dendritic cells to the autoimmune pathology of systemic lupus erythematosus

Noncoding RNAs and Their Potential Therapeutic Applications in Tissue Engineering

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