Retroviral mediated gene transfer into bone marrow progenitor cells: use of beta-galactosidase as a selectable marker

Strair, Roger; Towle, Murray J.; Smith, Brian R.

doi:10.1093/nar/18.16.4759

Cited by 26 publications

(12 citation statements)

References 14 publications

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“…However, these approaches have proven technically challenging because of the difficulty of using A/J as a background for transgenic embryos and because of the refractory nature of myeloid cells to transfection (data not shown; Chisholm and Symonds 1988;Rupprecht and Coleman 1991;Wright and Farber 1991;Stacey et al 1993;Nicolet and Paulnock 1994). The use of a substitute permissive transgenic background or the use of viral vectors may facilitate future analysis of Naip2 and Naip5 by functional complementation (Strair et al 1990;Haddada et al 1993).…”

Section: High-resolution Genetic and Physical Map Of Thementioning

confidence: 99%

High-resolution Genetic and Physical Map of theLgn1Interval in C57BL/6J ImplicatesNaip2orNaip5inLegionella pneumophilaPathogenesis

Growney¹,

Dietrich²

2000

Genome Res.

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Prior genetic and physical mapping has shown that theNaip gene cluster on mouse chromosome 13D1-D3 contains a gene, Lgn1, that is responsible for determining the permissivity of ex vivo macrophages to Legionella pneumophila replication. We have identified differences in the structure of the Naip array among commonly used inbred mouse strains, although these gross structural differences do not correlate with differences in L. pneumophilapermissiveness. A physical map of the region employing clones of the C57BL/6J haplotype confirms that there are fewer copies ofNaip in this strain than are in the physical map of the 129 haplotype. We have also refined the genetic location ofLgn1, leaving only Naip2 and Naip5as candidates for Lgn1. Our genetic map suggests the presence of two hotspots of recombination within the Naiparray, indicating that the 3′ portion of Naip may be involved in the genomic instability at this locus.[The sequence data described in this paper have been submitted to the GenBank data library under accession nos. AF240489–AF240530.]

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Section: High-resolution Genetic and Physical Map Of Thementioning

confidence: 99%

High-resolution Genetic and Physical Map of theLgn1Interval in C57BL/6J ImplicatesNaip2orNaip5inLegionella pneumophilaPathogenesis

Growney¹,

Dietrich²

2000

Genome Res.

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“…As for cytochemical markers, such as alkaline phosphatase and bacterial ␤-galactosidase, relatively high endogenous enzyme activity in some cell types and the necessity for substrate loading into the cytoplasm have limited their use. 12,13 Cell surface markers, such as the low affinity nerve growth factor receptor and CD24, have allowed rapid and efficient selection of transduced cells, 8,14 although they have the potential of eliciting unintended biological effects in vivo, as well as raising antibodies to eliminate gene-modified cells if they express foreign surface antigens.…”

Section: Introductionmentioning

confidence: 99%

Long-term tracking of murine hematopoietic cells transduced with a bicistronic retrovirus containing CD24 and EGFP genes

Kume

Ueda

et al. 2000

Gene Ther

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Hematopoietic stem cells (HSCs) are attractive targets for gene therapy, but current gene transfer methodologies are inadequate for efficient HSC transduction and perpetual transgene expression. To improve gene transfer vectors and transduction protocols, it is vital to establish a system to evaluate transgene expression and the long-term behavior of transduced cells in vivo. For this purpose, we constructed a bicistronic retrovirus encoding the human CD24 (as the first cistron) and the enhanced green fluorescent protein (EGFP; as the second cistron). Murine bone marrow cells were transduced with this vector and the transgene expression was monitored along with hematopoietic reconstitution. Stable expression of CD24 and EGFP was demon-

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“…FACS analysis has been used to enrich cells stably transfected with the P-Gal gene driven by various promoters (24)(25)(26)(27)(28). We adapted this procedure for use with cells transiently transfected with a ,3-Gal expression vector (pCH110), as well as pLTRluc, containing the full-length MMTV LTR-driving luciferase, and the PR expression vector, as shown in Fig.…”

mentioning

confidence: 99%

Newly expressed progesterone receptor cannot activate stable, replicated mouse mammary tumor virus templates but acquires transactivation potential upon continuous expression.

Smith

Archer

Hamlin-Green

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

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During development and differentiation, the expression of banscription factors is regulated in a temporal fashion. Newly expressed transcription factors must interact productively with target genes organized in chromatin. Although the mechanisms governing factor binding to chromatin templates are not well understood, it is now clear that template access can be dramatically influenced by nudeoprotein structure. We have examined the ability of a well characterized ranwactivator, the progesterone receptor (PR), to activate the mouse mammna tumor virus (MMTV) promoter organized either in stable, replicating templates that have a highly ordered nucleosome structure or as transiently transfected DNA, which adopts a less-dermed structure. (8). In this structural configuration, nuclear factor 1 (NF1) is excluded from its target site in the proximal promoter (1, 6, 9, 10). Binding of the activated glucocorticoid receptor (GR) to the promoter induces a chromatin remodeling event associated with the second nucleosome (Nuc-B) (8, 11). In addition, histone Hi is depleted from the promoter proximal region in a hormone-dependent manner (12). We proposed previously that this chromatin transition is directly and mechanistically involved in the binding of NF1 and subsequent formation of the transcription preinitiation complex (9, 10). Similar phenomena have been observed for the murine tyrosine aminotransferase (13-15) and yeast phoS (2,16) genes.The progesterone receptor (PR) and GR activate the MMTV promoter through the same target sequences as determined by transient transfections (17)(18)(19)(20)

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Retroviral mediated gene transfer into bone marrow progenitor cells: use of beta-galactosidase as a selectable marker

Cited by 26 publications

References 14 publications

High-resolution Genetic and Physical Map of theLgn1Interval in C57BL/6J ImplicatesNaip2orNaip5inLegionella pneumophilaPathogenesis

High-resolution Genetic and Physical Map of theLgn1Interval in C57BL/6J ImplicatesNaip2orNaip5inLegionella pneumophilaPathogenesis

Long-term tracking of murine hematopoietic cells transduced with a bicistronic retrovirus containing CD24 and EGFP genes

Newly expressed progesterone receptor cannot activate stable, replicated mouse mammary tumor virus templates but acquires transactivation potential upon continuous expression.

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