Retroviral microarray-based platform on nanostructured TiO2 for functional genomics and drug discovery

Carbone, Roberta; Giorgetti, Luca; Zanardi, Andrea; Marangi, Ida; Chierici, Elisabetta; Bongiorno, G.; Fiorentini, F.; Faretta, Mario; Piseri, P.; Pelicci, Pier Giuseppe; Milani, P.

doi:10.1016/j.biomaterials.2006.12.026

Cited by 31 publications

(27 citation statements)

References 41 publications

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“…S1). Additionally, ns-TiO x films showed a high biocompatibility with primary and cancer cell lines [40], [41], a good efficacy in protein immobilization [42], and the possibility of tailoring their physical and chemical properties by controlling the cluster assembling parameters [39], [43]. After annealing at 250°C films are hydrophilic (contact angle cosθ = 0.9) [39].…”

Section: Resultsmentioning

confidence: 99%

The Effect of Surface Nanometre-Scale Morphology on Protein Adsorption

et al. 2010

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BackgroundProtein adsorption is the first of a complex series of events that regulates many phenomena at the nano-bio interface, e.g. cell adhesion and differentiation, in vivo inflammatory responses and protein crystallization. A quantitative understanding of how nanoscale morphology influences protein adsorption is strategic for providing insight into all of these processes, however this understanding has been lacking until now.Methodology/Principal FindingsHere we introduce novel methods for quantitative high-throughput characterization of protein-surface interaction and we apply them in an integrated experimental strategy, to study the adsorption of a panel of proteins on nanostructured surfaces. We show that the increase of nanoscale roughness (from 15 nm to 30 nm) induces a decrease of protein binding affinity (≤90%) and a relevant increase in adsorbed proteins (≤500%) beyond the corresponding increase of specific area. We demonstrate that these effects are caused by protein nucleation on the surface, which is promoted by surface nanoscale pores.Conclusions/SignificanceThese results show that the adsorption of proteins depends significantly on surface nanostructure and that the relevant morphological parameter regulating the protein adsorption process is the nanometric pore shape. These new findings improve our understanding of the role of nanostructures as a biomaterial design parameter and they have important implications for the general understanding of cell behavior on nanostructured surfaces.

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Section: Resultsmentioning

confidence: 99%

The Effect of Surface Nanometre-Scale Morphology on Protein Adsorption

et al. 2010

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“…However, those earlier studies were limited by the technologies that only allowed analyzing a few genes at one time. The microarray techniques generate a quantitative analysis of over a thousand genes simultaneously [3][4][5][6][7], which have been used in many fields such as toxicity research [8,9], identification of genes relevant to diseases [10], filter of drugs [11,12] and in evaluation of biocompatibility [13][14][15][16]. Nowadays, the molecular biocompatibility study of biomaterials in genome scales has become possible with the introduction of the microarray technology [5][6][7]17,18].…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of cytotoxicity of nickel ions based on gene expression profiles

et al. 2009

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“…Cluster-assembled titania surfaces has been recently demonstrated as a very reach playground to study the influence of nanostructure on proteins and cells [56], [57], [58], [59].…”

Section: Introductionmentioning

confidence: 99%

Nanoscale Roughness and Morphology Affect the IsoElectric Point of Titania Surfaces

et al. 2013

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We report on the systematic investigation of the role of surface nanoscale roughness and morphology on the charging behaviour of nanostructured titania (TiO2) surfaces in aqueous solutions. IsoElectric Points (IEPs) of surfaces have been characterized by direct measurement of the electrostatic double layer interactions between titania surfaces and the micrometer-sized spherical silica probe of an atomic force microscope in NaCl aqueous electrolyte. The use of a colloidal probe provides well-defined interaction geometry and allows effectively probing the overall effect of nanoscale morphology. By using supersonic cluster beam deposition to fabricate nanostructured titania films, we achieved a quantitative control over the surface morphological parameters. We performed a systematical exploration of the electrical double layer properties in different interaction regimes characterized by different ratios of characteristic nanometric lengths of the system: the surface rms roughness Rq, the correlation length ξ and the Debye length λD. We observed a remarkable reduction by several pH units of IEP on rough nanostructured surfaces, with respect to flat crystalline rutile TiO2. In order to explain the observed behavior of IEP, we consider the roughness-induced self-overlap of the electrical double layers as a potential source of deviation from the trend expected for flat surfaces.

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Retroviral microarray-based platform on nanostructured TiO2 for functional genomics and drug discovery

Cited by 31 publications

References 41 publications

The Effect of Surface Nanometre-Scale Morphology on Protein Adsorption

The Effect of Surface Nanometre-Scale Morphology on Protein Adsorption

Mechanisms of cytotoxicity of nickel ions based on gene expression profiles

Nanoscale Roughness and Morphology Affect the IsoElectric Point of Titania Surfaces

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