1998
DOI: 10.1089/hum.1998.9.5-737
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Retroviral Vector Targeting to Melanoma Cells by Single-Chain Antibody Incorporation in Envelope

Abstract: Two strategies for targeting recombinant retroviruses to melanoma cells were compared. One was to extend the tropism of an ecotropic envelope to human melanoma cells, the other was to enhance the tropism of an amphotropic envelope for melanoma cells. Chimeric retroviral envelopes, incorporating a single-chain antibody (ScFv) directed against high-molecular-weight melanoma-associated antigen (HMWMAA) at the amino terminus are correctly processed and incorporated into virions. ScFv-ecotropic envelope chimeras al… Show more

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Cited by 49 publications
(35 citation statements)
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“…TELCeB6, TELCeB6/AF-7 and TELCeB6/GAF are TE671-derived packaging cells producing helper free, MLV-based retro-virus vector particles which encode the MFGnlslacZ genome and bear no MLV-A and GALV envelope glycoproteins, respectively. 7,23,42 A HT1080 based packaging cell line, FLYA4 lacZ 3 was also used. 7 Antibodies Goat polyclonal anti-MLV RT, anti-RLV P30 and anti-RLV gp69/71 (Quality Biotech Inc, Camden, USA) were used to detect MLV Gag and Env proteins.…”
Section: Cells and Virusesmentioning
confidence: 99%
“…TELCeB6, TELCeB6/AF-7 and TELCeB6/GAF are TE671-derived packaging cells producing helper free, MLV-based retro-virus vector particles which encode the MFGnlslacZ genome and bear no MLV-A and GALV envelope glycoproteins, respectively. 7,23,42 A HT1080 based packaging cell line, FLYA4 lacZ 3 was also used. 7 Antibodies Goat polyclonal anti-MLV RT, anti-RLV P30 and anti-RLV gp69/71 (Quality Biotech Inc, Camden, USA) were used to detect MLV Gag and Env proteins.…”
Section: Cells and Virusesmentioning
confidence: 99%
“…10,18 A number of reports, however, have indicated that low levels of targeted transduction may be achievable by coexpression of an ecotropic envelope in conjunction with the modified envelope protein. 7,9,16,19 To circumvent the problems associated with envelope modification, we have developed an alternative strategy, exploiting the natural budding mechanism of the virus to insert new binding ligands into the viral surface. We describe here successful and efficient specific targeting of transduction to human cells expressing stem cell factor (SCF) receptor (c-kit) by ecotropic retroviruses bearing surface stem cell factor.…”
Section: Introductionmentioning
confidence: 99%
“…As both 5D5 and 3D6 scFvs have similar binding affinities for the c-Met receptor, epitope specificity and/or scFv structure is probably critical for virus entry. Thus, in addition to the choice of the targeted cell-surface proteins, 15,16,19,20 our results show that that different ligands for a same receptor are not equivalent for retrovirus targeting. The choice of ligand for engineering the virus envelope is critical for the success of gene delivery.…”
Section: Discussionmentioning
confidence: 82%
“…Interestingly, these chimeric viruses preferentially infected the target tumors cells at high titer. 19,20 We recently demonstrated that retroviruses bearing hepatocyte growth factor/scatter (HGF) as a N-terminal extension of the envelope TM subunit, coexpressed with the amphotropic wild-type envelope, bind to the c-Met receptor and infect primary mouse and simian hepatocytes more efficiently than an unmodified virus. 21 This suggests the cooperative binding of the modified virus to c-Met via the HGF moiety and to Ram-1 via the SU subunit.…”
mentioning
confidence: 99%