1990
DOI: 10.1038/bjc.1990.304
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Retrovirus mediated transfer and expression of GM-CSF in haematopoietic cells

Abstract: Summary Two retrovirus vectors were compared for their ability to express granulocyte-macrophage colony stimulating factor (GM-CSF) in a haematopoietic cell line, FDCPI, which is dependent on GM-CSF for survival. Both a MoMLV-based vector pVneoGM, and a MPSV-based vector, M3neoGM, were found to be capable of transmitting and expressing both GM-CSF and neomycin sequences in the myeloid FDCPI cell line. Our results also demonstrate that pVneoGM is more efficient at generating GM-CSF independent colonies than M3n… Show more

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Cited by 10 publications
(2 citation statements)
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References 32 publications
(39 reference statements)
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“…This observation was confirmed using a colony-forming assay with mouse bone marrow cells (results not shown). The concentration curves shown in Figure 2 are in accordance with concentrations described to be needed for optimal stimulation of the FDCP-1 cell line (200-800 ng/ml 30 ). Freezing/thawing or incubation for 3 days at 37°C, 5% CO 2 (not shown) did not affect the results indicating that mGM-CSF produced was as stable as commercially available mGM-CSF.…”
Section: Determination Of the Biological Activity Of Gm-csf Produced supporting
confidence: 77%
“…This observation was confirmed using a colony-forming assay with mouse bone marrow cells (results not shown). The concentration curves shown in Figure 2 are in accordance with concentrations described to be needed for optimal stimulation of the FDCP-1 cell line (200-800 ng/ml 30 ). Freezing/thawing or incubation for 3 days at 37°C, 5% CO 2 (not shown) did not affect the results indicating that mGM-CSF produced was as stable as commercially available mGM-CSF.…”
Section: Determination Of the Biological Activity Of Gm-csf Produced supporting
confidence: 77%
“…The successful application of retroviruses as gene transfer vectors in the mammalian hematopoietic system is dependent on efficient infection by the retrovirus as well as stable integration and adequate expression of an unrearranged provirus. In vitro, retroviruses have been used to reliably transfer and express exogenous genes in a variety of hematopoietic cells, including undifferentiated progenitor cell lines (26,27,29). However, in undifferentiated hematopoietic cells in vivo, the utility of retroviral vectors in a number of investigations has been impaired by low levels of expression and/or lack of sustained expression of exogenous genes from viral long terminal repeats (LTRs) (2,9,16,24,35,56).…”
mentioning
confidence: 99%