Retting and degumming of natural fibers by pectinolytic enzymes produced from Bacillus tequilensis SV11-UV37 using solid state fermentation

Chiliveri, Swarupa Rani; Koti, Sravanthi; Linga, Venkateswar Rao

doi:10.1186/s40064-016-2173-x

Cited by 64 publications

(49 citation statements)

References 31 publications

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“…B. cereus achieved the same results as the ramie degumming technology currently used in the industry (such as CXJZ-120). The results were close to the effects of applying that bacterial strain for the degumming of kenaf (Chiliveri et al, 2016;Liu et al, 2012). Compared with other degumming bacterial strains (Basu et al, 2009;Zheng et al, 2001), B. cereus has a shorter degumming cycle (10 h) and ideal gum removal capabilities (residual gum rate is less than 6 %) and produces fiber of good quality (breaking strength is more than 850 cN dtex −1…”

Section: Discussionsupporting

confidence: 66%

Screening a bacterium and its effect on the biological degumming of ramie and kenaf

Cheng

Wang

Feng

et al. 2018

Sci. agric. (Piracicaba, Braz.)

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ABSTRACT:Biological degumming is an eco-friendly, efficient, high-quality and low-cost method that has become the leading bast fiber degumming technology. However, bacterial strains with short degumming cycles, high gum removal rates and small fiber damage are few. To screen high quality microbial resources with bast-fiber biological degumming function, soil samples were collected from a continuously cultivated banana plantation and then used to be enriched by ramie and kenaf materials in turn. A selective pectin-degrading medium was used to screen for excellent bacteria. A dominant bacterial strain was identified by phenotypic and genotypic characteristics, and its biological degumming effects on ramie and kenaf were verified by a comprehensive evaluation system. Results showed that seven bacterial strains secreting pectinase were obtained and the largest hydrolysis circle with a diameter ratio H/C of 2.4 was produced by the bacterial strain hn1-1, which was preliminarily identified as the Bacillus cereus by colony morphological characteristics and 16S rDNA sequence (GenBank accession number: KX013542) cluster analysis. The fiber production of ramie and kenaf degummed by B. cereus hn1-1 for 10 h were 72 % and 76 %, the residual gum rates were 4 % and 5 %, respectively. These values satisfied the textile industry requirement of < 6 % residual gum rate. Therefore, an effective biological degumming bacterium, B. cereus, was identified using a pectin-hydrolysis selective medium through a simple, economical, and time-saving method. Furthermore, the biological degumming technology by B. cereus for ramie and kenaf had a short cycle, ideal removal gum rate, and high-quality and productive fiber output.

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Section: Discussionsupporting

confidence: 66%

Screening a bacterium and its effect on the biological degumming of ramie and kenaf

Cheng

Wang

Feng

et al. 2018

Sci. agric. (Piracicaba, Braz.)

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“…performed the optimization of pectinase by response surface methodology using carrot peel powder, also stated the analysis of variance revealed high coefficient of determination (R 2 ) of 0.963 at p < 0.05 significant level. Chiliveri et al . studied the optimization of pectinase production by Bacillus tequilensis SV11‐UV37 in SSF using wheat bran as substrate through response surface methodology with coefficient of determination (R 2 ) of 0.99 at p < 0.05 significant level.…”

Section: Resultsmentioning

confidence: 99%

“…In accordance with these, Ghazala et al 42 performed the optimization of pectinase by response surface methodology using carrot peel powder, also stated the analysis of variance revealed high coefficient of determination (R 2 ) of 0.963 at p < 0.05 significant level. Chiliveri et al 43 44 reported the amylase production by Bacillus amyloliquefaciens KCP2 using wheat bran substrate in solid state fermentation through RSM with coefficient of determination (R 2 ) of 0.99 at p < 0.05 significant level. CCD helps to understand the interactive consequences among the tested variables and requires the lesser experimental runs.…”

Section: Optimization Of Enzymes Production By Traditional Methodsmentioning

confidence: 99%

“…In accordance with these, performed the optimization of pectinase by response surface methodology and stated increased level of pectinase at carrot peels powder 6.5%, NH 4 Cl 3.0 g kg −1 , inoculum level 3% by Bacillus mojavensis I4. 42 Chiliveri et al 43 documented the statistical optimization of pectinase production and optimum process parameters for Bacillus tequilensis SV11-UV37 were: 20% (v/w) Inoculum, 3% (w/w) pectin and 80% (v/w) moisture content of wheat bran. The predicted response for amylase production was 786 U g −1 and the analytical value was 944 U g −1 , thereby validating the designed CCD model.…”

Section: Validation Of the Designed Ccd Modelmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous production of commercial enzymes using agro industrial residues by statistical approach

Viayaraghavan¹,

Kumar²,

Arasu

et al. 2019

J Sci Food Agric

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BACKGROUND Simultaneous production of commercial enzymes using agro‐industrial residues by statistical approach is an important perspective in an industrial point of view. Despite the advantages of statistical methods optimization, the report on simultaneous production of pectinase and amylases are limited. The accumulation of agro‐industrial residues causes serious environmental problems; however, citrus peel can be the important substrate for various enzymes production, including pectinase. These enzymes involving saccharification process and act as clarifying agent. RESULTS In this study, orange peel and banana peel mixture were used as the suitable substrate for pectinase and amylase production using Bacillus pumilus in solid‐state culture. The process parameters were optimized for simultaneous production of enzymes by a traditional‐one‐variable‐at‐a‐time approach, a two level full factorial design, central composite design and response surface methodology. Among the selected variables, moisture content of the medium, pH and mineral supplement significantly influenced pectinase and amylase production. Pectinase production increased over 3‐fold, whereas, 2‐fold increase on amylase production was achieved after optimization by statistical approach. The purified pectinase exhibited maximal activity at pH 8.0, temperature of 60 °C and the molecular weight was 60 kDa. The purified amylase was highly active at pH 8.0, at 50 °C and the molecular weight was 37 kDa. The enzyme showed activity on fruit pulp in increasing clarity in orange and carrot juice and the saccharification of starch. CONCLUSION Orange peel and banana peel mixture was effective as a solid medium for the simultaneous production of pectinase and amylase by Bacillus pumilus. Also, our statistical approach to optimize the medium components to yield more pectinase and amylase was fruitful and these enzymes showed appreciable results suitable for various applications. © 2018 Society of Chemical Industry

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“…Specifically for fiber use, problems hinge around issues of scale and economy, in which the retting and decortication process has been described as a "bottleneck" for supplying consistent product to industry manufacturers [2,4,20,21]. Research addressing this problem has focused broadly on two areas: alternatives to field-retting such as solid-state fermentation with specific organisms and post-harvest treatments that focus on improving the end product without regard to the logistical and economic considerations, or adjusting agronomic practices such as timing of planting and harvest, turning in the field, and variety selection [6,15,17,[22][23][24]. Fungal species are predicted to play a role in retting, by both their own degradation abilities and the process by which they facilitate entry of surface microorganisms past the cuticle, and have been a target for attempts to manipulate retting outcomes [17,25].…”

Section: Discussionmentioning

confidence: 99%

The Microbiology of Hemp Retting in a Controlled Environment: Steering the Hemp Microbiome towards More Consistent Fiber Production

2020

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Industrial hemp (Cannabis sativa L.) production is increasing dramatically in the US due to recent changes which lift restrictions on the growth and sale of hemp products; however, due to the decades-long prohibition of hemp, there is a lack of current research with respect to varieties and best agricultural practices for the many uses of this versatile crop. Natural fiber production relies on retting, a microbially-mediated process necessary for the separation of fibers from the plant which can occur unevenly in the field environment and result in inconsistent fiber quality and lower processing efficiency. In this study, the microbiome of hemp stalks is investigated throughout the retting process using 16S rRNA gene amplicon sequencing using the Illumina MiSeq platform. Field retting conditions were simulated in a controlled greenhouse environment in order to determine the effects of different moisture levels and soil contact on the retting process. Samples were taken over six time points, reflecting the community of freshly cut stalks to optimally-retted material, and finally over-retted material showing degraded fibers. The results show a very consistent population throughout retting, dominated primarily by Proteobacteria, but showing an increase in the abundance of the Bacteroidetes, namely Chryseobacterium, in time points corresponding to optimally-retted and over-retted stalks in treatments receiving higher moisture levels, but not in the low-moisture treatment. Soil application did not appear to influence the microbial community throughout retting, indicating a resilient population present in and on the hemp stalks at harvest.

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Retting and degumming of natural fibers by pectinolytic enzymes produced from Bacillus tequilensis SV11-UV37 using solid state fermentation

Cited by 64 publications

References 31 publications

Screening a bacterium and its effect on the biological degumming of ramie and kenaf

Screening a bacterium and its effect on the biological degumming of ramie and kenaf

Simultaneous production of commercial enzymes using agro industrial residues by statistical approach

The Microbiology of Hemp Retting in a Controlled Environment: Steering the Hemp Microbiome towards More Consistent Fiber Production

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