Reusable antimicrobial antibiotic-free dressings obtained by photopolymerization

Cagnetta, Gonzalo Emanuel; Martínez, Sol R.; Ibarra, Luis Exequiel; Gallastegui, Antonela; Martucci, Josefa Fabiana; Palacios, Rodrigo E.; Chesta, Carlos A.; Gómez, María Lorena

doi:10.1016/j.bioadv.2023.213399

Cited by 9 publications

(12 citation statements)

References 43 publications

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“…The main objective of these experiments was to investigate how bacterial viability could be affected by exposing the microbial culture to the copolymer hydrogel, keeping the sample in the dark, or irradiating it with visible light, where radical species could be generated. The monomer METAC was chosen to introduce positive charges into the polymeric matrix, thus imparting antimicrobial activity to the hydrogel, as we have recently demonstrated in the development of dermatological wound dressings . The experimental setup for the PDI essays is schematized in Figure C.…”

Section: Resultsmentioning

confidence: 99%

“…As shown, a small decrease in cell viability is observed (<1 log, * p < 0.05 compared to the light control), which means that the hydrogel is slightly toxic to the microorganism in the dark. This is a typical bacteriostatic profile and is attributed to the cationic monomer METAC, which destabilizes the overall negative cell wall charge and thus inhibits cell replication . Interestingly, the microorganism treated with the hydrogel plus light (red circles) displays an abrupt inactivation of 5 logs after 30 min of irradiation when compared with the same hydrogel in dark conditions.…”

Section: Resultsmentioning

confidence: 99%

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Conjugated Polymer Nanoparticles as Visible Light Panchromatic Photoinitiators for 3D Printing of Acrylic Hydrogels

Cagnetta,

Gallastegui,

Martínez

et al. 2023

Macromolecules

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Conjugated polymers (CPs) are known to generate radical reactive oxygen species (ROS) such as hydrogen peroxide through photoinduced charge transfer processes involving water. In this work, this ability to generate radicals is utilized here to use conducting polymer nanoparticles (CPNs) as photoinitiator systems (PIS) for the polymerization of acrylic monomers in aqueous media. The CPNs do not require co-initiators and also act as cross-linkers. CPNs with different light-absorption properties effectively initiate photopolymerization across the entire visible light spectrum, reaching high monomer conversion yields (60− 99%) under blue, green, or red LED exposure. The resulting hydrogels demonstrate remarkable characteristics, including a significant water absorption swelling capacity (900−1600%) and substantial gel formation percentages (70−97%). Notably, the CPNs are evenly dispersed within the hydrogel matrix after photopolymerization, maintaining their photophysical properties and their ability to generate reactive species, potentially rendering the hydrogel suitable for bacterial photodynamic inactivation. Furthermore, PIS CPNs enable 3D printing with a fine resolution of up to 100 μm using a digital light processing technique.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Conjugated Polymer Nanoparticles as Visible Light Panchromatic Photoinitiators for 3D Printing of Acrylic Hydrogels

Cagnetta,

Gallastegui,

Martínez

et al. 2023

Macromolecules

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“…This versatility makes them promising tools for advancing the frontiers of personalized medicine and improving clinical outcomes. CPNs have also been used as photoinitiators of vinyl polymerization in aqueous media, , allowing the synthesis of hydrogels with a wide variety of clinical applications . In many of these applications, CPNs are deliberately loaded, (either volumetrically or superficially) with synthetic small-molecule dyes to tune their photophysical properties.…”

Section: Introductionmentioning

confidence: 99%

Unraveling the Adsorption Mechanism of Rhodamine B onto Conjugated Polymeric Nanoparticles Using Isothermal Calorimetry and Monte Carlo Simulations

Ponzio,

Odella,

Prost

et al. 2024

J. Phys. Chem. C

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We present herein a comprehensive study on the thermodynamics of rhodamine B (RhB) adsorption onto poly(9,9dioctylfluorene-alt-benzothiadiazole) (F8BT) nanoparticles (NPs) dispersed in water using the isothermal calorimetric titration (ITC) technique. ITC experiments were carried out over a wide temperature range (5−65 °C), and the resulting thermograms were subjected to a detailed thermodynamic analysis using different adsorption models. For this purpose, we developed a versatile computational code based on the Monte Carlo method. We found that the thermograms obtained at the different temperatures cannot be interpreted using the Langmuir, bi-Langmuir, or Brunauer, Emmet, Teller (BET) models. However, excellent thermogram fits were attained by applying the Langmuir model coupled with the formation of a RhB dimer on the surface of F8BT NPs. Using this model, the best fit to the experimental data at 25 °C yielded the following parameters for direct RhB adsorption on the F8BT NPs surface: equilibrium constant (K l ) of ∼2 × 10 7 M −1 , enthalpy change (ΔH l ) of ∼1 kJ mol −1 , and entropy change (ΔS l ) of ∼140 J K −1 mol −1 . These results indicate that dye adsorption is predominantly an entropy-controlled process. The fit also provided parameters associated with the formation of a second dye adsorption layer, i.e., the formation of the RhB dimer on the F8BT NPs surface, revealing values of ∼1.7 × 10 5 M −1 , ca. −20 kJ mol −1 , and ∼30 J K −1 mol −1 for K m , ΔH m , and ΔS m , respectively. The latter two parameters are similar to those previously reported for RhB dimer formation in water (ΔH d = −12 kJ mol −1 and ΔS d = 22 J K −1 mol −1 ) supporting the chosen Langmuir-dimer model. The analysis of the thermograms also suggests that the NPs surface available for RhB adsorption is small, as compared to the total surface, and that it decreases with decreasing temperature. The latter observation is intriguing, especially since the F8BT NPs hydrodynamic diameter shows only a small decrease with decreasing temperature. To explain these results, we propose that the surface of the NPs harbors highly hydrated polar groups that reduce the accessibility of the dye for adsorption.

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“…However, this presents an opportunity for alternative therapies, such as metallic nanoparticles, to emerge as antibiotic‐free solutions 15 . These alternative therapies have associated toxicity concerns, but this presents an opportunity for natural extracts with antimicrobial properties and cationic compounds capable of disrupting microorganisms' membranes to emerge as antibiotic‐free solutions 16‐21 to become the next generation of antimicrobials.…”

Section: Introductionmentioning

confidence: 99%

“…Silsesquioxanes, either alone or incorporated into polymeric matrices, have proven to be suitable materials in biomedical applications 23 . Hydrogels synthesized containing functional silsesquioxanes, synthesized by photopolymerization, were employed by our group for the development of bioinspired filters, drug delivery systems and dermatological skin dressings tested in vitro 21,24‐26 . These dressings were developed using SSOF‐3, as a crosslinker, which proved to be a novel choice not previously employed in dressing scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional skin dressings synthesized via one‐pot photopolymerization: Advancing wound healing and infection prevention strategies

Zambroni,

Martínez,

Cagnetta

et al. 2024

Polymers for Advanced Techs

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In the field of wound healing treatment, the development of new materials is essential to cover multiple functions, such as acceleration of the healing process, prevention of infection and response to stimuli, especially if they present lower costs or are easier to manufacture. These properties are of great importance to prompt skin cicatrization. In this work, new multifunctional skin dressings for wound healing and infection prevention are developed based on new hydrogel materials. The dressings are produced via a straightforward and environmentally friendly one‐pot photopolymerization manufacturing process utilizing vitamin B2 aqueous solutions as sensitizer, and energy‐efficient blue light sources. These dressings exhibit a high swelling capacity, pH and temperature responsiveness, biocompatibility, antimicrobial activity in the absence of conventional antibiotics, and are capable of promoting wound healing. The formulation is based on the combination of three monomers: 2‐((methacryloyloxy)ethyl)trimethylammonium chloride, methacrylic acid and N‐vinyl caprolactam. Collagen, hyaluronic acid, and essential oil of hop are added to enhance antimicrobial properties and stimulate regenerative cellular processes. In vivo experiments have demonstrated that pristine dressings are superior in promoting collagen and fibroblast regeneration and accelerating cicatrization as compared to dressings containing natural products. The dressing embedded with hop also displayed antimicrobial characteristics. The preparation and testing of these new efficient wound dressings are described. This study is distinctive in its integration of environmentally conscious manufacturing techniques with innovative material formulations, resulting in superior wound dressings that are both cost‐effective and highly functional. By achieving enhanced healing and infection prevention without the employment of conventional antibiotics, this work represents a substantial advancement in sustainable and effective wound care management.

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Reusable antimicrobial antibiotic-free dressings obtained by photopolymerization

Cited by 9 publications

References 43 publications

Conjugated Polymer Nanoparticles as Visible Light Panchromatic Photoinitiators for 3D Printing of Acrylic Hydrogels

Conjugated Polymer Nanoparticles as Visible Light Panchromatic Photoinitiators for 3D Printing of Acrylic Hydrogels

Unraveling the Adsorption Mechanism of Rhodamine B onto Conjugated Polymeric Nanoparticles Using Isothermal Calorimetry and Monte Carlo Simulations

Multifunctional skin dressings synthesized via one‐pot photopolymerization: Advancing wound healing and infection prevention strategies

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