Reused cultivation water from a self-inhibiting alga does not inhibit other algae but alters their microbiomes

Loftus, Sarah E.; Hunt, Dana E.; Johnson, Zackary I.

doi:10.1016/j.algal.2020.102067

Cited by 7 publications

(5 citation statements)

References 66 publications

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“…We found correlations and putative associations between chlorophyte (green algal) OTUs and OTUs of several bacteria identified by other studies to be chlorophyte microbiome constituents, including families Comamonadaceae , Rhodobacteraceae , and Chthoniobacteraceae [95, 96], and Flavobacteriaceae and order Sphingomonadales (Alphaproteobacteria) [97]. Correlations were also identified between chlorophytes and sulfur bacteria (family Chromatiaceae ) which are known to be abundant in lakes [98], and cyanobacteria of order Oscillatoriales (family Microcoleaceae ), and two families of potentially pathogenic Gammaproteobacteria, Moraxellaceae and Aeromonadaceae [99, 100].…”

Section: Discussionmentioning

confidence: 78%

“…We found a diatom (phylum Ochrophyta , class Bacillariophyta ) of order Aulacoseirales that was significantly correlated several other bacterial and eukaryotic OTUs: one ciliate, a bacterium of order Spirochaetales which is known to be present among some diatom microbiota [96], cyanobacteria of orders Chroococcales and Synechococcales , one OTU of order Verrucomicrobiales , and an Alphaproteobacterium of order Rickettsiales . Several of the observed bacterial associations, in particular cyanobacteria of orders Chroococcales and Synechococcales , may be related to their shade-tolerance during dense blooms of diatoms and other eukaryotes [104, 105].…”

Section: Discussionmentioning

confidence: 99%

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Time-series metagenomics reveals changing protistan ecology of a temperate dimictic lake

Krinos,

Bowers,

Rohwer

et al. 2024

Preprint

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Protists, single-celled eukaryotic organisms, are critical to food web ecology, contributing to primary productivity and connecting small bacteria and archaea to higher trophic levels. Lake Mendota is a large, eutrophic natural lake that is a Long-Term Ecological Research site and among the world's best-studied freshwater systems. Metagenomic samples have been collected and shotgun sequenced from Lake Mendota for the last twenty years. Here, we analyze this comprehensive time series to infer changes to the structure and function of the protistan community, and to hypothesize about their interactions with bacteria. Based on small subunit rRNA genes extracted from the metagenomes and metagenome-assembled genomes of microeukaryotes, we identify shifts in the eukaryotic phytoplankton community over time, which we predict to be a consequence of reduced zooplankton grazing pressures after the invasion of a invasive predator (the spiny water flea) to the lake. The metagenomic data also reveal the presence of the spiny water flea and the zebra mussel, a second invasive species to Lake Mendota, prior to their visual identification during routine monitoring. Further, we use species co-occurrence and co-abundance analysis to connect the protistan community with bacterial taxa. Correlation analysis suggests that protists and bacteria may interact or respond similarly to environmental conditions. Cryptophytes declined in the second decade of the timeseries, while many alveolate groups (e.g. ciliates and dinoflagellates) and diatoms increased in abundance, changes that have implications for food web efficiency in Lake Mendota. We demonstrate that metagenomic sequence-based community analysis can complement existing efforts to monitor protists in Lake Mendota based on microscopy-based count surveys. We observed patterns of seasonal abundance in microeukaryotes in Lake Mendota that corroborated expectations from other systems, including high abundance of cryptophytes in winter and diatoms in fall and spring, but with much higher resolution than previous surveys. Our study identified long-term changes in the abundance of eukaryotic microbes, and provided context for the known establishment of an invasive species that catalyzes a trophic cascade involving protists. Our findings are important for decoding potential long-term consequences of human interventions, including invasive species introduction.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Time-series metagenomics reveals changing protistan ecology of a temperate dimictic lake

Krinos,

Bowers,

Rohwer

et al. 2024

Preprint

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“…More specifically, Staurosira sp. biomass production decreased by more than 50% in recycled media compared to fresh media [15]. Interestingly, Auxenochlorella protothecoides, an auxotroph that requires thiamine supplementation, was able to utilize the spent media generated by Chlorella sorokiniana, which contains thiamine metabolites that alleviated thiamine deficiency [61].…”

Section: Effect Of Media Recycling On Algal Growth and Biochemical Co...mentioning

confidence: 99%

“…Following algal harvesting, around 84% of the water is discharged, while the remainder is lost to evaporation [13]. Media recycling helps reduce water use and also enables nutrient recycling, as significant amounts of residual nutrients are still present in spent media, but growth inhibition is often reported in recycled media and needs to be addressed [14,15]. According to life cycle assessment (LCA) research, reusing media could minimize freshwater and nutrient use by 84% and 55%, respectively [13].…”

Section: Introductionmentioning

confidence: 99%

A Critical Review of Growth Media Recycling to Enhance the Economics and Sustainability of Algae Cultivation

Arora,

Lo,

Legall

et al. 2023

Energies

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Microalgae hold promise as a sustainable source of biofuels and bioproducts but their commercial development is impeded by high cultivation costs, primarily for growth nutrients, and concerns about the water-intensive nature of algae cultivation. As a result, minimizing water and nutrient input is imperative to reducing algal operating costs, while enhancing the sustainability of future algal biorefineries. However, spent media recycling often results in the accumulation of growth inhibitors, such as free fatty acids, polysaccharides, polyunsaturated aldehydes, and humic acid, which negatively affect algal growth and productivity. In this review, we critically assess media recycling research findings to assess the advantages and disadvantages of spent media reuse for a wide range of algae strains. Particular emphasis is placed on strategies to overcome growth inhibition through spent media treatment processes, such as ultraviolet oxidation, activated carbon, ultrasonication, microfiltration, crop rotation, and nutrient replenishment.

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“…However, overcoming the opposing influence of reused water for MC involves pretreatment methods for inhibitory reused water and crop rotation [49]. In addition, the responses of algae growth in water reuse systems may largely rely on strain-specific factors [50].…”

Section: Water Reuse For Microalgae Cultivationmentioning

confidence: 99%

A Critical Review of Recent Progress in Global Water Reuse during 2019–2021 and Perspectives to Overcome Future Water Crisis

Abou-Shady,

Siddique,

2023

Environments

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The exacerbation of the global water crisis due to an increase in global population, industrialization, urbanization, and agricultural activities, along with global climate change and limited water resources, makes water reuse inevitable in all continents. By 2030, global water consumption may grow to ~160% of the currently available volume. This study reviews recently published articles (2019–2021) to explore global case studies of water reuse and discusses future perspectives by country based on a literature survey on water reuse. There are 17 obstacles reported worldwide regarding water reuse (e.g., the properties and low amounts of treated water, regulations, financial challenges, etc.) and 10 advantages of utilizing reused water in various fields (e.g., overcoming the global water crisis, improving the economy, benefiting the industrial sector, etc.). The concept of reusing water has been accepted by countries in almost every continent (e.g., Australia, Europe, Asia, Africa, South America, and North America); the technical findings from different countries are summarized in this study. The water reuse scenario is not restricted to countries with limited water supply and can be applied to those with sufficient water resources (e.g., Canada and Brazil have also implemented water reuse policies). Water reuse can be utilized by human beings via indirect and direct potable recycling, as well as in agriculture, textile, construction, hotel, groundwater recharge, and aquaculture industries. However, a standard guideline for the application of reclaimed water at a global scale is unavailable. Several perspectives have been suggested for the future utilization of reclaimed water worldwide as an effort to secure and ensure the sustainability of existing natural water resources. Lastly, water reuse may be considered a potential alternative for reducing the burden on water resources in the future.

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Reused cultivation water from a self-inhibiting alga does not inhibit other algae but alters their microbiomes

Cited by 7 publications

References 66 publications

Time-series metagenomics reveals changing protistan ecology of a temperate dimictic lake

Time-series metagenomics reveals changing protistan ecology of a temperate dimictic lake

A Critical Review of Growth Media Recycling to Enhance the Economics and Sustainability of Algae Cultivation

A Critical Review of Recent Progress in Global Water Reuse during 2019–2021 and Perspectives to Overcome Future Water Crisis

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