Reutilização de implantes de progesterona: do teste in vitro às taxas de concepção na IATF.

Abstract

Aos meus pais, avós e irmãs, Ao Hermano DEDICO AGRADEÇO Ao meu pai, Udo Kehrle, bons conselhos, pela imensa bondade que tem dentro do coração, pelo bom humor. Um dos maiores responsáveis por tudo o que eu sou.À minha mãe, Zuleika Provasi, que sempre apóia minhas decisões, mesmo quando estas envolvem quilômetros de distância. Ela me criou para o mundo, e incentiva minha coragem.Às minhas irmãs, Karen (Oiii meu amoooor) e Ellen (levantando uma sobrancelha). Já estou rindo!! Aos meus avós Erika e George, semeadores da minha profissão. The aim of this study was to characterize progesterone devices and their reuse and to evaluate its efficiency. Three experiments were conducted: to assess the quantities of released P4 (in vitro trial), plasma levels provided (in vivo trialovariectomized cows) and conception rates at FTAI (field trial -suckled beef cows).In vitro trial, the three reuses of devices were tested in a tablet dissolutor with six tanks, with a solution of alcohol / water (60:40) as way of dissemination. Two trials were conducted, totaling 12 implants. The third use of the device was added with a P4 ring (0.1 g) and in the fourth use two rings were added (0.1 g each). The new implants were tested in quadruplicate in a third trial, using the same methodology. , 4, 6, 8, 10, 12, 24, 48, 72, 96, 120, 144, 168 and 192h. The dosages of P4 were performed by Radio-Immuno-Assay -RIA, using a commercial kit from DPC (Diagnostics Products Corporation, USA). The area under the curve differed between the first and second (p <0.0001), second and third (p = 0.0010) but not between the third and fourth use (p = 0.8764). For the statistical analysis of these experiments, the PROC IML, GLM (command REPEATED) and REG (for in vitro test, which generated a regression equation) or the t test (for the area under the curve assessment -in vivo test), were used