Revealing a novel natural bioflocculant resource from Ruditapes philippinarum: Effective polysaccharides and synergistic flocculation

Mu, Jun; HaiJun, Zhou; Yin, Changqing; Yang, Guangfeng; Cui, Xia

doi:10.1016/j.carbpol.2018.01.036

Cited by 32 publications

(32 citation statements)

References 29 publications

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“…Twelve kinds of monosaccharides occurred with a varied proportion in these strains, including Man, GlcN, Rib, Rha, GlcUA, GalUA, GalN, Glc, Gal, Xyl, Ara and Fuc (S3 Table). A Heat-map as Fig 7 was constructed for comparing monosaccharide composition of EPS from bioflocculant-producing isolates with flocculation-active polysaccharides RPMP-1 and RPMP-2 from RPM[9]. It’s shown that RPMP-1 was highly similar in monosaccharide composition to GHS19 and GHS20, while RPMP-2 was similar in monosaccharide composition to GHS8-1, though both of them still shared common monosaccharide types with the other strains.…”

Section: Resultsmentioning

confidence: 99%

“…In our previous studies, RPM was discovered to be a promising natural bioflocculant resource showing excellent flocculation and decoloration activities[9, 11]. Naturally collected RPM exhibits high flocculation activities to kaolin clay both in deionized water and sea water assay systems, and also could flocculate marine microalgae[9]. Further research triggered speculation that bioflocculant activities of RPM might be attributed to R .…”

Section: Discussionmentioning

confidence: 99%

“…Ruditapes philippinarum conglutination mud (RPM), which refers particularly to the settled sludge from R . philippinarum when freshly harvested clams are kept in sterilized seawater for mud spitting, has been recently reported as a promising natural bioflocculant resource[9]. RPM possesses good flocculation capability, decoloration and heavy metal removal activity, especially exhibits easy recovery and fast settlement before and after treating pollutants[9–11].…”

Section: Introductionmentioning

confidence: 99%

“…It is a necessity to characterize the microbial community structure of RPM and identify its associated bioflocculant-producing microbes. Additionally, since the flocculation-active polysaccharides of RPM have been recently reported for their composition[9], comparing the chemical composition of EPS of flocculant-producing microbial strains with RPM polysaccharides will give sound proof for judging the origin of RPM bioflocculation components.…”

Section: Introductionmentioning

confidence: 99%

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Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China

Mu¹,

Cui²,

Shao³

et al. 2019

PLoS ONE

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Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R . philippinarum and has been recently reported as a promising natural bioflocculant resource. However the origin of bioflocculation components within RPM is still a pending doubt and impedes its effective exploitation. This study investigated the probability that RPM bioflocculation components originate from its associated microbes. RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components. Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade , CL500_29_marine_group , Fusibacter , MWH_UniP1_aquatic_group and Arcobacter as the dominant genera. Fourteen highly efficient bioflocculant-producing strains were screened and phylogenetically identified as Pseudoalteromonas sp. (5), Psychrobacter sp. (3), Halomonas sp. (2), Albirhodobacter sp. (1), Celeribacter sp. (1), Kocuria sp. (1) and Bacillus sp. (1), all of which except Bacillus sp. were reported for the first time for their excellent flocculation capability. Furthermore, EPS from the bioflocculant-producing strains exhibited highly similar monosaccharide composition to the reported flocculation-effective RPM polysaccharides. On the other hand, the existence of fungi in RPM was rare and showed no flocculation functionality. Findings from Zhoushan RPM strongly supported that RPM flocculation components were of bacterial origin and make RPM reproduction possible by fermentation approach.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China

Mu¹,

Cui²,

Shao³

et al. 2019

PLoS ONE

Self Cite

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“…Bioflocculants are needed to replace fossil-fuel based flocculants such as Polyacrylamide and poly(ethylene oxide) for industrial applications [13]. Several research studies are searching for effective strains that can be cultivated in low cost medium with maximum bioflocculant production rates [14,15]. The objectives of research were to screen fungi for the production of bioflocculants and the conditions that will enhance its optimum production by the test fungi.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of Fungal Bioflocculants and Its Application in Water Treatment

David

Oluwole

Ayodele

et al. 2019

CJAST

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Bioflocculants of microbial origin have the advantage of being safe, biodegradable and harmless to the environment. Production of bioflocculant by two fungi isolates and the factors affecting its production were investigated in this study. Primary screening of fungi for the production of bioflocculants, efficiencies and conditions for the optimum production of the bioflocculants were determined using standard microbiological and chemical methods. Aspergillus flavus MCB 271 and Aspergillus niger MCBF 08 were the best bioflocculant producers among the fourteen fungal isolates screened. Aspergillus flavus optimally produced bioflocculant with glucose and peptone as sole carbon and nitrogen sources respectively. Calcium ions (Ca2+) at 78.4% served as best cation sources for bioflocculant production with optimal pH of 7 and temperature of 40°C. Aspergillus niger MCBF 08 produced bioflocculant optimally when the media had peptone as a nitrogen source and maltose as a sole carbon source. The two species achieved the maximum flocculating activity of 97% (A. flavus MCBF 271) and 86% (A. niger MCBF 08) at pH values of 7 on the 3rd day of the study and caused a reduction in bacterial load of the wastewater samples by 58.73% and 60.85% respectively. These bioflocculants are thus potential replacement for synthetic flocculants conventionally used for wastewater treatment.

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Flocculation–solidification combined method for treatment of hydraulically dredged mud at extra high water content

Zhang

Zheng

et al. 2019

Acta Geotech.

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Revealing a novel natural bioflocculant resource from Ruditapes philippinarum: Effective polysaccharides and synergistic flocculation

Cited by 32 publications

References 29 publications

Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China

Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China

Characterisation of Fungal Bioflocculants and Its Application in Water Treatment

Flocculation–solidification combined method for treatment of hydraulically dredged mud at extra high water content

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