Revealing Nanoscale Membrane Curvature with Polarized Localization Microscopy

Maarouf, A.; Meerschaert, Rebecca L.; Kelly, Christopher V.

doi:10.1364/ntm.2015.nm3c.2

Cited by 1 publication

(2 citation statements)

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“…The size distribution of mitochondria and ER organelles, the diffusion of individual dye molecules within these structures, and the fusion and fission of the mitochondrial and ER membranes was queried with STORM using lipophilic membrane dyes . The restricted orientation of lipophilic dyes in the lipid bilayer can also be used to examine the orientation and topology of the membrane using fluorescent molecule localization under polarized light . This scheme is an extension of polarized total internal reflection fluorescence microscopy (pTIRFM), which is able to differentiate between membrane orientations by selecting dye molecules based on their alignment with a polarized TIRF excitation field …”

Section: Super-resolution Investigations Of Biological Membranesmentioning

confidence: 99%

“…123 The restricted orientation of lipophilic dyes in the lipid bilayer can also be used to examine the orientation and topology of the membrane using fluorescent molecule localization under polarized light. 124 This scheme is an extension of polarized total internal reflection fluorescence microscopy (pTIRFM), which is able to differentiate between membrane orientations by selecting dye molecules based on their alignment with a polarized TIRF excitation field. 125 2.2.2.…”

Section: Membrane Organizational Modesmentioning

confidence: 99%

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Super-Resolution Microscopy: Shedding Light on the Cellular Plasma Membrane

2017

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Lipids and the membranes they form are fundamental building blocks of cellular life, and their geometry and chemical properties distinguish membranes from other cellular environments. Collective processes occurring within membranes strongly impact cellular behavior and biochemistry, and understanding these processes presents unique challenges due to the often complex and myriad interactions between membrane components. Super-resolution microscopy offers a significant gain in resolution over traditional optical microscopy, enabling the localization of individual molecules even in densely labeled samples and in cellular and tissue environments. These microscopy techniques have been used to examine the organization and dynamics of plasma membrane components, providing insight into the fundamental interactions that determine membrane functions. Here, we broadly introduce the structure and organization of the mammalian plasma membrane and review recent applications of super-resolution microscopy to the study of membranes. We then highlight some inherent challenges faced when using super-resolution microscopy to study membranes, and we discuss recent technical advancements that promise further improvements to super-resolution microscopy and its application to the plasma membrane.

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Section: Super-resolution Investigations Of Biological Membranesmentioning

confidence: 99%