Revealing non-genetic adhesive variations in clonal populations by comparative single-cell force spectroscopy

Dao, Lu; Weiland, Ulrich; Nazarenko, Irina; Kalt, H.; Bastmeyer, Martin; Franz, Clemens M.

doi:10.1016/j.yexcr.2012.06.017

Cited by 17 publications

(19 citation statements)

References 58 publications

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“…Moreover, they point into evidence that HGG shows a cell-cell adhesion profile (both for detachment force and work) more uniform than that observed for the LGG. The cell adhesion variability is a feature already observed in SCFS data and it was demonstrated that this behavior does not depend on cell cycle phase, but originates predominantly from cell to cell variations [44]. However, in our measurements this variability for the LGG could be also associated with the elastic properties of Gsc.…”

Section: Discussionsupporting

confidence: 58%

Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy

et al. 2014

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Active cell migration and invasion is a peculiar feature of glioma that makes this tumor able to rapidly infiltrate into the surrounding brain tissue. In our recent work, we identified a novel class of glioma-associated-stem cells (defined as GASC for high-grade glioma -HG- and Gasc for low-grade glioma -LG-) that, although not tumorigenic, act supporting the biological aggressiveness of glioma-initiating stem cells (defined as GSC for HG and Gsc for LG) favoring also their motility. Migrating cancer cells undergo considerable molecular and cellular changes by remodeling their cytoskeleton and cell interactions with surrounding environment. To get a better understanding about the role of the glioma-associated-stem cells in tumor progression, cell deformability and interactions between glioma-initiating stem cells and glioma-associated-stem cells were investigated. Adhesion of HG/LG-cancer cells on HG/LG-glioma-associated stem cells was studied by time-lapse microscopy, while cell deformability and cell-cell adhesion strengths were quantified by indentation measurements by atomic force microscopy and single cell force spectroscopy. Our results demonstrate that for both HG and LG glioma, cancer-initiating-stem cells are softer than glioma-associated-stem cells, in agreement with their neoplastic features. The adhesion strength of GSC on GASC appears to be significantly lower than that observed for Gsc on Gasc. Whereas, GSC spread and firmly adhere on Gasc with an adhesion strength increased as compared to that obtained on GASC. These findings highlight that the grade of glioma-associated-stem cells plays an important role in modulating cancer cell adhesion, which could affect glioma cell migration, invasion and thus cancer aggressiveness. Moreover this work provides evidence about the importance of investigating cell adhesion and elasticity for new developments in disease diagnostics and therapeutics.

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Section: Discussionsupporting

confidence: 58%

Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy

et al. 2014

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“…The bond rupture force of CM integrins‐CN in our study (61.69 ± 5.5 pN) is slightly greater than theirs, but these results are comparable. Compared with detachment forces between CHO cells integrins‐LN quantified by Dao et al, the unbinding force of CM integrins‐LN (108.31 ± 4.2 pN, Figure 2) in our study is smaller than the one reported. The discrepancy may be due to different measuring methods (single‐cell force spectroscopy in the report vs single‐molecular force spectroscopy in the present study) and cell lines (CHO vs CM).…”

Section: Discussioncontrasting

confidence: 93%

Characterization of adhesion properties of the cardiomyocyte integrins and extracellular matrix proteins using atomic force microscopy

Liu

Yang

et al. 2019

J of Molecular Recognition

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Integrins are transmembrane adhesion receptors that play important roles in the cardiovascular system by interacting with the extracellular matrix (ECM). However, direct quantitative measurements of the adhesion properties of the integrins on cardiomyocyte (CM) and their ECM ligands are lacking. In this study, we used atomic force microscopy (AFM) to quantify the adhesion force (peak force and mean force) and binding probability between CM integrins and three main heart tissue ECM proteins, ie, collagen (CN), fibronectin (FN), and laminin (LN). Functionalizing the AFM probes with ECM proteins, we found that the peak force (mean force) was 61.69 ± 5.5 pN (76.54 ± 4.0 pN), 39.26 ± 4.4 pN (59.84 ± 3.6 pN), and 108.31 ± 4.2 pN (129.63 ± 6.0 pN), respectively, for the bond of CN‐integrin, FN‐integrin, and LN‐integrin. The binding specificity between CM integrins and ECM proteins was verified by using monoclonal antibodies, where α10‐ and α11‐integrin bind to CN, α3‐ and α5‐integrin bind to FN, and α3‐ and α7‐integrin bind to LN. Furthermore, adhesion properties of CM integrins under physiologically high concentrations of extracellular Ca2+ and Mg2+ were tested. Additional Ca2+ reduced the adhesion mean force to 68.81 ± 4.0 pN, 49.84 ± 3.3 pN, and 119.21 ± 5.8 pN and binding probability to 0.31, 0.34, 0.40 for CN, FN, and LN, respectively, whereas Mg2+ caused very minor changes to adhesion properties of CM integrins. Thus, adhesion properties between adult murine CM integrins and its main ECM proteins were characterized, paving the way for an improved understanding of CM mechanobiology.

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“…However, the deadhesion work was not significantly higher for plasma‐treated glass over steam‐treated glass, indicating subtle differences in the way the cells responded to the array of micro‐ und nanotopographic features. The single‐cell measurements also revealed a variation of force values between individual cells and between individual force measurements, which could be due to the statistical distribution of the sterilization‐induced protrusions in the glass surface and well‐established variations in adhesion within cell populations …”

Section: Resultsmentioning

confidence: 99%

“…The single-cell measurements also revealed a variation of force values between individual cells and between individual force measurements, which could be due to the statistical distribution of the sterilization-induced protrusions in the glass surface and well-established variations in adhesion within cell populations. 22 Enhanced adhesion on plasma and gamma treated glass coincided with increased average roughness of these surfaces compared to steam treated glass. To test for a possible correlation of cell adhesion strength and surface roughness, we plotted maximal adhesion force and detachment work versus the average overall surface roughness [ Fig.…”

Section: Correlation Of Adhesion Strength and Surface Roughnessmentioning

confidence: 90%

Nanoscale topographic changes on sterilized glass surfaces affect cell adhesion and spreading

Wittenburg

Lauer

Oswald

et al. 2013

J Biomedical Materials Res

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Producing sterile glass surfaces is of great importance for a wide range of laboratory and medical applications, including in vitro cell culture and tissue engineering. However, sterilization may change the surface properties of glass and thereby affect its use for medical applications, for instance as a substrate for culturing cells. To investigate potential effects of sterilization on glass surface topography, borosilicate glass coverslips were left untreated or subjected to several common sterilization procedures, including low-temperature plasma gas, gamma irradiation and steam. Imaging by atomic force microscopy demonstrated that the surface of untreated borosilicate coverslips features a complex landscape of microislands ranging from 1000 to 3000 nm in diameter and 1 to 3 nm in height. Steam treatment completely removes these microislands, producing a nanosmooth glass surface. In contrast, plasma treatment partially degrades the microisland structure, while gamma irradiation has no effect on microisland topography. To test for possible effects of the nanotopographic structures on cell adhesion, human gingival fibroblasts were seeded on untreated or sterilized glass surfaces. Analyzing fibroblast adhesion 3, 6, and 24 h after cell seeding revealed significant differences in cell attachment and spreading depending on the sterilization method applied. Furthermore, single-cell force spectroscopy revealed a connection between the nanotopographic landscape of glass and the formation of cellular adhesion forces, indicating that fibroblasts generally adhere weakly to nanosmooth but strongly to nanorough glass surfaces. Nanotopographic changes induced by different sterilization methods may therefore need to be considered when preparing sterile glass surfaces for cell culture or biomedical applications.

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Revealing non-genetic adhesive variations in clonal populations by comparative single-cell force spectroscopy

Cited by 17 publications

References 58 publications

Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy

Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy

Characterization of adhesion properties of the cardiomyocyte integrins and extracellular matrix proteins using atomic force microscopy

Nanoscale topographic changes on sterilized glass surfaces affect cell adhesion and spreading

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