Revealing real-time 3D in vivo pathogen dynamics in plants by label-free optical coherence tomography
Jos de Wit,
Sebastian Tonn,
Mon-Ray Shao
et al.
Abstract:Microscopic imaging for studying plant-pathogen interactions is limited by its reliance on invasive histological techniques, like clearing and staining, or, for in vivo imaging, on complicated generation of transgenic pathogens. We present real-time 3D in vivo visualization of pathogen dynamics with label-free optical coherence tomography. Based on intrinsic signal fluctuations as tissue contrast we image filamentous pathogens and a nematode in vivo in 3D in plant tissue. We analyze 3D images of lettuce downy … Show more
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