Revealing sex-specific molecular changes in hypoxia-ischemia induced neural damage and subsequent recovery using zebrafish model

Hypoxia induced sex-difference in zebrafish brain proteome profile reveals the crucial role of H3K9me3 in recovery from acute hypoxia

“…Throughout the protein enrichment analysis by IPA sex-specific global proteome changes in the acute hypoxia zebrafish model was observed, which is in concurrence with our previous study [16]. But the question which remains unsolved is why the recovery in female quicker than in male.…”

“…In male most of the increased activation state was observed in organismal injury, abnormalities, cell death, connective tissue disorders, developmental disorder, skeletal and muscular disorders, neurological, respiratory diseases and cancer; whereas in female cellular assembly and organization, cellular function and maintenance, cell morphology, nucleic acid metabolism, cellular movement showed an increased activation state. The disease function analysis clearly showed a sex-specific difference in hypoxia-induced neural damage and recovery as seen in our earlier studies [16].…”

“…As described previously by us [16], 4 hours post-acute hypoxia treatment (for 5 min at DO = ± 0.6 mg/liter) the brain tissues from both hypoxia-treated and untreated male and female zebrafish were isolated (n=6 per group) and subjected to comprehensive proteomic profiling. For this, male and female zebrafish brains of nine individuals from each gender were pooled together for iTRAQ based LC-MS/MS method and three technical replicates were run in LC-MS/MS, as depicted in Fig.…”

“…Sex-specific differences in the hypoxic-ischemic brain have profound implications for effective prevention and treatment. Global hypoxic-ischemic damages and recovery are well studied under the well-controlled, non-invasive, reproducible conditions in zebrafish [13][14][15][16][17]. In our previous study, we have reported the sex-specific difference in hypoxia-induced neural damage and recovery, where we have concluded that as compared to male, female showed higher level of neural damage and an ability to recover faster.…”

Molecular Basis of Sex Difference in Neuroprotection induced by Hypoxia Preconditioning in Zebrafish

“…The expression of neuron-specific class III ß-tubulin (TuJ1), a neural differentiation marker, gets enriched in absence of transcription factor HIF1α, as reported in neural stem cell and mouse model (Bohuslavova et al, 2019;Vecera et al, 2018). Here in our study we have observed a loss of Tuj1 expression in both acute hypoxia groups (AHM and AHF) as compared to the corresponding normoxia groups, which confers with the onset of hypoxia in AH groups Hif 1 α expression is more as reported earlier (Das et al, 2019) which resulted in loss of Tuj1 expression, irrespective of sex. But in preconditioning (PH) groups since the system was already adapted to hypoxia and under the protective effect, Hif 1 alpha expression would be less than that in AH resulting in increase of Tuj1 expression as compared to AH.…”