Revealing the 1H NMR metabolome of mirasol chili peppers (Capsicum annuum) infected by Candidatus Phytoplasma trifolii

Velásquez‐Valle, Rodolfo; Villa‐Ruano, Nemesio; Hidalgo-Martínez, Diego; Zepeda, L. Gerardo; Pérez‐Hernández, Nury; Reyes-López, César A.; Reyes-Cervantes, Eric; Medina-Melchor, Diana Laura; Becerra‐Martínez, Elvia

doi:10.1016/j.foodres.2019.108863

Cited by 17 publications

(6 citation statements)

References 37 publications

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“…The extraction and identification of capsaicin and dihydrocapsaicin were done by RP-HPLC in accordance with Velasquez-Valle et al [31] using the same equipment and analytical conditions reported by these authors. The identity of these molecules was confirmed with authentic standards (see chemicals section).…”

Section: Determination Of Capsaicinoids In Cera Peppersmentioning

confidence: 99%

Peppermint Essential Oil and Its Major Volatiles as Protective Agents against Soft Rot Caused by Fusarium sambucinum in Cera Pepper (Capsicum pubescens)

Pérez-Vázquez

Hernández

Lozoya‐Gloria

et al. 2021

Chemistry & Biodiversity

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Cera pepper (Capsicum pubescens) is an exotic fruit considered as a rich source of nutraceuticals with known benefits for human health and also an economic resource for local producers in Mexico. The present investigation reports on the in vitro and in situ antifungal activity of the essential oil from Mentha piperita and its two major volatiles (menthol and menthone) against Fusarium sambucinum, which is a causal agent of soft rot in cera pepper. The application of these components in pepper fruits previously infected with F. sambucinum caused a significant delay (p < 0.05) in the emergence of soft rot symptoms. This effect was reflected in the maintenance of pH and fruit firmness during a period of 10 days. The nutrimental content of the fruits (protein, fiber, fat and other proximate parameters) was conserved in the same period of time. The nutraceutical content of these fruits was estimated by the quantification of seven carotenoids (violaxanthin, cis-violaxanthin, luteoxanthin, antheraxanthin, lutein, zeaxanthin and β-carotene), ascorbic acid and capsaicinoids (capsaicin and dihydrocapsaicin). According to our results, the essential oil from M. Piperita and its major volatiles exerted a preservative effect on these metabolites. Our findings demonstrated that the essential oil of M. Piperita and its major volatiles represent an ecological alternative for the control of fusariosis caused by F. sambucinum in cera peppers under postharvest conditions.

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Section: Determination Of Capsaicinoids In Cera Peppersmentioning

confidence: 99%

Peppermint Essential Oil and Its Major Volatiles as Protective Agents against Soft Rot Caused by Fusarium sambucinum in Cera Pepper (Capsicum pubescens)

Pérez-Vázquez

Hernández

Lozoya‐Gloria

et al. 2021

Chemistry & Biodiversity

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“…Their findings revealed overall similar metabolic changes in the leaves under viral stress, which is consistent with our results. Various metabolite markers have been identified in different studies that investigated the alteration of plant metabolome under geminivirus infection (López-gresa et al, 2012; Villa-ruano et al, 2018; Velásquez-valle et al, 2020; Donati et al, 2022). Additionally, a similar set of 1H NMR peaks for legume plant metabolome has been previously reported in several studies (Chen et al, 2019; Bueno and Lopes, 2020; Villa-ruano et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…There exists a wealth of research conducted on the altered metabolic state of entire symptomatic plant leaves during virus infection using metabolomics (Choi et al, 2006; Lionel et al, 2012; Mandal et al, 2012; López-Gresa et al, 2012; Palama et al, 2012; Plischke et al, 2012; Manchester and Anand, 2017; Villa-Ruano et al, 2018; Rossouw et al, 2019; Velásquez-Valle et al, 2020; J. Mishra et al, 2020; Maravi et al, 2022; More et al, 2022; Zhang et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Identification of Begomovirus Genomic Components and Stress-specific Metabolic Markers in Mungbean Leaf Apoplast

Dhobale,

Sahoo

2023

Preprint

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Plant viruses exert control over the host metabolism to enhance their survival, but the specific sites where altered metabolites take effect remain enigmatic. This study focuses on the apoplastic region of symptomatic mungbean leaves infected with mungbean yellow mosaic India virus (MYMIV) to gain new insights into metabolite changes during infection. Leveraging NMR-based metabolome fingerprinting, we identified several stress-associated metabolites. Notably, proline and 2-Hydroxybutyrate were found to be up-regulated in the apoplast region, while down-regulated in the cytosol. Additionally, aspartate was found to be upregulated in the cytosolic region but absent in the apoplast. Importantly, our molecular analysis of the apoplast suggests the presence of MYMIV genomic components. Moreover, we characterized two distinct populations of extracellular vesicles (EVs) using ultracentrifugation, transmission electron microscopy, dynamic light scattering, and fluorometric assays. The data revealed alterations in the size and count of EVs, suggesting their potential role in facilitating the transport of viral components for long-distance cell-to-cell movement during infection. These findings provide valuable insights into the apoplast's role and the significance of EVs in MYMIV infection, contributing to a better understanding of virus-host interactions and potentially informing new virus defense strategies.

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“…Gas chromatography–mass spectrometry (GC–MS) analyses of stip, a physiological disorder in bell pod-type peppers, detected significant variation in 13 metabolites related to fruit maturation and senescence ( Fulton et al, 2021 ). Metabolomic profiling of mirasol pepper fruit samples infected by Candidatus Phytoplasma trifolii showed decreased levels of fructose, glucose, and capsaicin, suggesting that infection can reduce the pungency and nutraceutical value of mirasol peppers ( Velásquez-Valle et al, 2020 ). Stress-related metabolite accumulation was further observed using a large-scale metabolomic characterization of mature fruit samples from 26 pepper cultivars ( Kim et al, 2020 ).…”

Section: The “Panomics” Platform: Integrating Multi-omics Tools For T...mentioning

confidence: 99%

Chile Pepper (Capsicum) Breeding and Improvement in the “Multi-Omics” Era

et al. 2022

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Chile pepper (Capsicum spp.) is a major culinary, medicinal, and economic crop in most areas of the world. For more than hundreds of years, chile peppers have “defined” the state of New Mexico, USA. The official state question, “Red or Green?” refers to the preference for either red or the green stage of chile pepper, respectively, reflects the value of these important commodities. The presence of major diseases, low yields, decreased acreages, and costs associated with manual labor limit production in all growing regions of the world. The New Mexico State University (NMSU) Chile Pepper Breeding Program continues to serve as a key player in the development of improved chile pepper varieties for growers and in discoveries that assist plant breeders worldwide. Among the traits of interest for genetic improvement include yield, disease resistance, flavor, and mechanical harvestability. While progress has been made, the use of conventional breeding approaches has yet to fully address producer and consumer demand for these traits in available cultivars. Recent developments in “multi-omics,” that is, the simultaneous application of multiple omics approaches to study biological systems, have allowed the genetic dissection of important phenotypes. Given the current needs and production constraints, and the availability of multi-omics tools, it would be relevant to examine the application of these approaches in chile pepper breeding and improvement. In this review, we summarize the major developments in chile pepper breeding and present novel tools that can be implemented to facilitate genetic improvement. In the future, chile pepper improvement is anticipated to be more data and multi-omics driven as more advanced genetics, breeding, and phenotyping tools are developed.

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Revealing the 1H NMR metabolome of mirasol chili peppers (Capsicum annuum) infected by Candidatus Phytoplasma trifolii

Cited by 17 publications

References 37 publications

Peppermint Essential Oil and Its Major Volatiles as Protective Agents against Soft Rot Caused by Fusarium sambucinum in Cera Pepper (Capsicum pubescens)

Peppermint Essential Oil and Its Major Volatiles as Protective Agents against Soft Rot Caused by Fusarium sambucinum in Cera Pepper (Capsicum pubescens)

Identification of Begomovirus Genomic Components and Stress-specific Metabolic Markers in Mungbean Leaf Apoplast

Chile Pepper (Capsicum) Breeding and Improvement in the “Multi-Omics” Era

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