Revealing the interaction between intrauterine adhesion and vaginal microbiota using high‑throughput sequencing

Abstract

Intrauterine adhesion (IUA) is one of the most common diseases of the reproductive system. Due to the high postoperative recurrence rate of IUA, it is crucial to identify the possible causes of pathogenesis and recurrence of this disease. In the present study, a high-throughput sequencing approach was applied to compare the vaginal microbiota between healthy women [healthy vaginal secretion (HVS) group] and patients with IUA [intrauterine adhesion patients' vaginal secretion (IAVS) group]. The results indicated that IUA had little effect on the number of vaginal bacterial species. However, at the phylum level, patients with IUA had a significantly lower percentage of Firmicutes and a higher percentage of Actinobacteria than the HVS group (P<0.05). At the genus level, ~50% of patients with IUA were found to have a marked reduction in probiotic Lactobacillus accompanied by an overgrowth of pathogenic Gardnerella and Prevotella (P<0.05), and the Principal Coordinates Analysis confirmed that 10/20 samples in the IAVS group were scattered far away from the HVS group. Therefore, it was concluded that the interaction between IUA and vaginal microbiota greatly influenced the vaginal diversity of patients with IUA. In order to increase the recovery rate and lower the recurrence rate of IUA, increasing the vaginal Lactobacillus population should be considered.

Revealing the Disturbed Vaginal Micobiota Caused by Cervical Cancer Using High-Throughput Sequencing Technology

“…Theαvalue was set to 0.05 in order to correct multiple comparisons. Finally, we used Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUST) to analyze the metabolic function and its influence on the host cell of bacteria in Kyoto Encyclopedia of Genes and Genomes (KEGG) functional spectrum database ( Liu et al., 2019 ; Zheng et al., 2019 ).…”

Mini-Review of the New Therapeutic Possibilities in Asherman Syndrome—Where Are We after One Hundred and Twenty-Six Years?

“…Another example is represented by the patients with IUAs where significant differences at the phylum and genus level were observed (more precisely, a significant reduction in Firmicutes and Lactobacillus , to the detriment of Actinobacteria, Gardnerella , and Prevotella [ 50 ]. The presence of, and subsequent infections caused by, Mycobacterium tuberculosis is also detrimental.…”

miR-543 Inhibits the Occurrence and Development of Intrauterine Adhesion by Inhibiting the Proliferation, Migration, and Invasion of Endometrial Cells

“…IUA refers to the injury of the basal layer of endometrium caused by pregnant or nonpregnant uterine trauma and even complete occlusion of the uterine cavity or cervix, such as secondary amenorrhea, periodic abdominal pain, recurrent abortion, and infertility [ 13 , 14 ]. Previous studies have confirmed that the expression of miR-543 is downregulated in IUA and negatively regulates CDH2 [ 7 ].…”