Revealing the mechanism of surfactant-promoted enzymatic hydrolysis of dilute acid pretreated bamboo

Elucidating the nonproductive adsorption mechanism of cellulase with lignin fractions from hydrothermally pretreated poplar using multi-dimensional spectroscopic technologies

“…According to previous studies, realtime monitoring of the dynamic adsorption process between SL, RL and cellulase was performed using an SPR instrument (MP-SPR Navi 200, Finland). 32,33 Briefly, a lignin film was prepared by spin coating onto Au chips. Before passing the cellulase solution for SPR analysis, sodium citrate buffer (0.05 M) was passed (0.1 mL min −1 ) to acquire a stable baseline.…”

“…These data imply the greater adsorption of RL than SL to cellulase. 33,34 Subsequently, the buffer was passed through to simulate the desorption process, at which point the SPR response value (RU) decreased, indicating that desorption of cellulase from SL and RL occurred. 57 Compared with RL, the RU value of the SL-cellulase system decreased more obviously.…”

“…However, research on the interaction between lignin and cellulase using SPR is limited. In recent years, researchers, such as Lin et al , 32 Huang et al , 33 and Zhao et al , 9 have pioneered the use of SPR to explore the kinetic parameters of the interaction between lignin and cellulase, as well as the interaction between surfactants and lignin-cellulase and pseudo-lignin-cellulase systems. Pereira et al 34 conducted enzyme adsorption experiments using SPR in buffer solutions with different ionic strengths to assess the influence of electrostatic interactions between lignin and cellulase.…”

Valorization of bamboo shoot shell waste for the coproduction of fermentable sugars and xylooligosaccharides

“…The results revealed that the enzymatic hydrolysis efficiency increased significantly after the addition of PEG/Tween 80 to the enzymatic system of pretreated BSS (170 °C, 50 min), which could achieve a yield of 86.6% (Tween 80) and 88.4% (PEG). It has been reported that the addition of surfactants could reduce the adsorption ability of lignin for enzymes, resulting in freer cellulase in the system for hydrolysis of the cellulose substrate (Huang et al, 2022c). Overall, the enzymatic hydrolysis efficiency of BSS improved substantially after autohydrolysis, and the optimization of temperature and duration further improved it.…”

Improved Synthesis of Furfurylamine from a High Titer of Biomass-Derived Furfural by a Thermostable Triple Mutant ω-Transaminase in a Three-Component Deep Eutectic Solvent ChCl/Lactic Acid/Malonic Acid System

“…Increasingly severe warming of the global climate and swift depletion of fossil fuels have stimulated the development of sustainable and renewable bioresources to replace the current fossil energy economy. − Cost-effective utilization of available renewable lignocellulose (LC) to manufacture valuable chemicals may alleviate the resource/energy crisis and environmental pollution. − Hemicellulose is an important polysaccharide in biomass, which can be used as feedstock for furfural (FAL) production . FAL is a versatile molecule that not only can be used to manufacture a series of furan derivatives, fine chemicals, biofuels, fuel additives, resins, plastics, fungicides, and nematicides , but also can be utilized as an industrial solvent for lubricating oil refining .…”