Revealing the Second and the Third Causes of AgNPs Property to Restore the Bacterial Susceptibility to Antibiotics

Bogdanchikova, Nina; Maklakova, Maria; Villarreal-Gómez, Luis Jesús; Нефедова, Е. В.; Shkil, N. N.; Plotnikov, Evgenii; Pestryakov, Alexey

doi:10.3390/ijms24097854

Cited by 3 publications

(11 citation statements)

References 37 publications

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“…In our previous work, it was shown that the anti-lysozyme activity of five antibiotics for S. epidermidis ATCC 14990 was 100%, but cultivation with AgNPs decreased the anti-lysozyme activity by 20%, which was a greater decrease than for the other six bacteria [17]. The highest decrease in the adhesion index was also observed for S. epidermidis after AgNP treatments [17]. Probably for S. epidermidis changes caused by AgNPs are not connected to efflux pumps but are rather connected to changes in the bacteria adhesion index and its anti-lysozyme activity.…”

Section: Change In the Percentage Of Isolates With Efflux Effectmentioning

confidence: 84%

“…although other virulence factors play an important role in the influence of AgNPs on susceptibility to antibiotics. In our previous work, it was shown that the anti-lysozyme activity of five antibiotics for S. epidermidis ATCC 14990 was 100%, but cultivation with AgNPs decreased the anti-lysozyme activity by 20%, which was a greater decrease than for the other six bacteria [17]. The highest decrease in the adhesion index was also observed for S. epidermidis after AgNP treatments [17].…”

Section: Change In the Percentage Of Isolates With Efflux Effectmentioning

confidence: 88%

“…bacteria [17]. The highest decrease in the adhesion index was also observed for S. epidermidis after AgNP treatments [17].…”

Section: Change In the Number Of Isolates Of Four Bacteria After Trea...mentioning

confidence: 89%

“…The cases of combined application of AgNPs with optimal antibiotics leading to synergetic effects help to fight multidrug-resistant bacteria. Our new approach (described in a series of our previous publications [10][11][12]17] and in the present work) consists of a non-simultaneous use of AgNPs and antibiotics, applying AgNPs in a pretreatment stage followed by the antibiotic treatment stage. Pretreatment (preliminary treatment) is an action that aims to improve the results of the following treatment.…”

Section: Cow Recovery Periodmentioning

confidence: 99%

“…Historically, the influence of AgNPs on the activity of antibiotics was carried out when they were applied together. Therefore, we did not find any article (published before the series of our work on this topic [10][11][12]17]) dedicated to the influence of animal pre-treatment with AgNPs on bacterial resistance to antibiotics. The results of the series of our works revealed that animal pre-treatment with AgNPs led to a decrease in the resistance of bacteria to antibiotics.…”

Section: Cow Recovery Periodmentioning

confidence: 99%

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Nanoparticles Partially Restore Bacterial Susceptibility to Antibiotics

Bogdanchikova,

Luna Vazquez-Gomez,

Nefedova

et al. 2024

Materials

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The growing resistance of bacteria to antibiotics is one of the main public health problems nowadays. The influence of silver nanoparticle (AgNP) pretreatment of 220 cows with mastitis on the susceptibility of Staphylococcus epidermidis bacteria to 31 antibiotics was studied. The obtained results were compared with the previous results for Escherichia coli, Streptococcus dysgalactiae, and Staphylococcus aureus. For all four bacteria, an increase in susceptibility (9.5–21.2%) to 31 antibiotics after cow treatment with AgNPs was revealed, while after first-line antibiotic drug treatment as expected, the susceptibility decreased (11.3–27.3%). These effects were explained by (1) the increase in the contribution of isolates with efflux effect after antibiotic treatments and its decrease after AgNP treatment and (2) the changes in bacteria adhesion and anti-lysozyme activity after these treatments. The effect of the increasing antibacterial activity of antibiotics after AgNP treatment was the most pronounced in the case of E. coli and was minimal in the case of S. epidermidis. With AgNP treatment, the time of recovery decreased by 26.8–48.4% compared to the time of recovery after treatment with the first-line antibiotic drugs. The AgNP treatment allows for achieving the partial restoration of the activity of antibiotics.

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Section: Change In the Percentage Of Isolates With Efflux Effectmentioning

confidence: 84%

Section: Change In the Percentage Of Isolates With Efflux Effectmentioning

confidence: 88%

“…bacteria [17]. The highest decrease in the adhesion index was also observed for S. epidermidis after AgNP treatments [17].…”

Section: Change In the Number Of Isolates Of Four Bacteria After Trea...mentioning

confidence: 89%

Section: Cow Recovery Periodmentioning

confidence: 99%

Section: Cow Recovery Periodmentioning

confidence: 99%

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Nanoparticles Partially Restore Bacterial Susceptibility to Antibiotics

Bogdanchikova,

Luna Vazquez-Gomez,

Nefedova

et al. 2024

Materials

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Role of Biofilm Formation in the Drop of Bacterial Resistance to Antibiotics after Animal Therapy with Silver Nanoparticles

Maklakova,

Villarreal-Gómez,

Nefedova

et al. 2024

ACS Appl. Nano Mater.

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Bacterial biofilm formation poses significant consequences in the treatment of infections, leading to increased virulence and antibiotic resistance of bacteria and evasion of immune responses. Various methods aimed at preventing biofilm formation were investigated, including the use of silver nanoparticles (AgNPs). However, the previous studies were focused on the efficacy of AgNPs in vitro. In this study, we investigated the effectiveness of AgNPs in vivo in treating 700 cows with subclinical mastitis. The results obtained with AgNP therapy were compared with results obtained with first-line antibiotic-containing drug therapy. The biofilm formation index was quantified by using the crystal violet test. The number of isolates with strong and weak biofilm formation activities was assessed. Our findings demonstrate that therapy with AgNPs resulted in a reduction in the average index of biofilm-forming activity of six bacteria by 20.4% for strong biofilms and by 16.4% for weak biofilms across six bovine mastitis-causing pathogens (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus dysgalactiae, Escherichia coli, Streptococcus agalactiae, and Streptococcus pyogenes). Conversely, treatment with antibiotic-containing drug led to a 40.4% increase in the average index of biofilm-forming activity for isolates with strong biofilms and an 8.7% increase for isolates with weak biofilms. Additionally, the number of strong biofilm isolates increased by an average of 232% after the antibiotic treatment. Therefore, our study identified the fourth reason for the recently revealed phenomenon of the decrease of bacterial resistance caused by animal therapy with AgNPs, highlighting as well the potential of AgNPs as a therapeutic intervention against biofilm-associated infections. This translational research represents a significant advancement from laboratory studies to real-world applications of AgNP therapy for the decrease of bacterial antibiotic resistance.

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Alginate Silver Nanoparticles and Their Effect on Sperm Parameters of the Domestic Rabbit

Rutkowski,

Grzesiakowska,

Kuchta-Gładysz

et al. 2024

Applied Sciences

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Silver nanoparticles possess valuable physical, chemical, and biological properties, rendering them widely applied as bioactive agents in the industry. Nonetheless, their influence on the natural environment and on living organisms remains unclear. Therefore, this study aims to investigate the impact of polymer composites containing silver nanoparticles on sperm cells. The nanosilver polymer composites were chemically synthesized, employing sodium alginate as the stabilizer. The reducing agents employed were solutions comprising sodium borohydride and xylose. The concentration of silver nanoparticles obtained after synthesis was 100 parts per million. The examined biological species were rabbit sperm cells. The impact of nanosilver on the sperm was assessed through the elucidation of the toxicity profile, comet test, and analysis of morphological characteristics of the animal cells. The results of the study demonstrate a twofold impact of polymer composites infused with silver nanoparticles on domestic rabbit sperm when obtained through chemical synthesis using two reducing agents (xylose and sodium borohydride) at a 10 ppm concentration. The comet test showed no harmful effect on the DNA integrity of rabbit sperm by the tested compounds. Twenty-four-hour exposure of rabbit spermatozoa to silver nanoparticles, obtained by reducing xylose and borohydride, induced significant secondary changes in the morphological structure of male reproductive cells. These findings indicate the potential reproductive toxicity of silver nanoparticles.

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Revealing the Second and the Third Causes of AgNPs Property to Restore the Bacterial Susceptibility to Antibiotics

Cited by 3 publications

References 37 publications

Nanoparticles Partially Restore Bacterial Susceptibility to Antibiotics

Nanoparticles Partially Restore Bacterial Susceptibility to Antibiotics

Role of Biofilm Formation in the Drop of Bacterial Resistance to Antibiotics after Animal Therapy with Silver Nanoparticles

Alginate Silver Nanoparticles and Their Effect on Sperm Parameters of the Domestic Rabbit

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