Revealing the secret lives of cryptic species: Examining the phylogenetic relationships of echinostome parasites in North America

Detwiler, Jillian T.; Bos, David H.; Minchella, Dennis J.

doi:10.1016/j.ympev.2010.01.004

Cited by 123 publications

(220 citation statements)

References 35 publications

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“…Molecular identification, such as we have used, and species ''barcoding'' are allowing the study of these larval forms in more detail (Locke et al, 2011), and more advanced microscopy, especially SEM, might be a powerful tool for observing additional morphological detail of larval forms. Care must be taken though, since several recent studies have demonstrated the presence of numerous cryptic species within common North American trematode families (Detwiler et al, 2010;Locke et al, 2010). Given the prior variation in life history reported across the range and the morphological differences that have been identified in adult M. oregonensis (Lang et al, 1968), and given our suggestion that variation in cercariae morphology seems to be present, the presence of multiple species within the M. oregonensis group remains a possibility that needs further exploration.…”

Section: Discussionmentioning

confidence: 99%

Metagonimoides oregonensis (Heterophyidae: Digenea) Infection In Pleurocerid Snails and Desmognathus quadramaculatus Salamander Larvae In Southern Appalachian Streams

Belden

Peterman

Smith

et al. 2012

Journal of Parasitology

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Section: Discussionmentioning

confidence: 99%

Metagonimoides oregonensis (Heterophyidae: Digenea) Infection In Pleurocerid Snails and Desmognathus quadramaculatus Salamander Larvae In Southern Appalachian Streams

Belden

Peterman

Smith

et al. 2012

Journal of Parasitology

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“…Three days prior to the start of the experiment, snails were acclimated to 25°C and fed rabbit chow ad libitum. We identified cercariae as Echinoparyphium lineage 3 using standard molecular sequencing of the ITS1 gene and implementation of Bayesian phylogenetics methods (Detwiler, Bos, & Minchella, 2010; Hua et al., 2016). …”

Section: Methodsmentioning

confidence: 99%

Evolved pesticide tolerance influences susceptibility to parasites in amphibians

Hua

Wuerthner

Jones

et al. 2017

Evolutionary Applications

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Because ecosystems throughout the globe are contaminated with pesticides, there is a need to understand how natural populations cope with pesticides and the implications for ecological interactions. From an evolutionary perspective, there is evidence that pesticide tolerance can be achieved via two mechanisms: selection for constitutive tolerance over multiple generations or by inducing tolerance within a single generation via phenotypic plasticity. While both mechanisms can allow organisms to persist in contaminated environments, they might result in different performance trade‐offs including population susceptibility to parasites. We have identified 15 wood frog populations that exist along a gradient from close to agriculture and high, constitutive pesticide tolerance to far from agriculture and inducible pesticide tolerance. Using these populations, we investigated the relationship between evolutionary responses to the common insecticide carbaryl and host susceptibility to the trematode Echinoparyphium lineage 3 and ranavirus using laboratory exposure assays. For Echinoparyphium, we discovered that wood frog populations living closer to agriculture with high, constitutive tolerance experienced lower loads than populations living far from agriculture with inducible pesticide tolerance. For ranavirus, we found no relationship between the mechanism of evolved pesticide tolerance and survival, but populations living closer to agriculture with high, constitutive tolerance experienced higher viral loads than populations far from agriculture with inducible tolerance. Land use and mechanisms of evolved pesticide tolerance were associated with susceptibility to parasites, but the direction of the relationship is dependent on the type of parasite, underscoring the complexity between land use and disease outcomes. Collectively, our results demonstrate that evolved pesticide tolerance can indirectly influence host–parasite interactions and underscores the importance of including evolutionary processes in ecotoxicological studies.

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“…Bray et al, 1999Bray et al, , 2009Detwiler et al, 2010;Curran et al, 2013;Georgieva et al, 2014;Selbach et al, 2014), mitochondrial large subunit rRNA (rrnL or 16S rrn) gene (e.g. Li et al, 2010;Leung et al, 2009;Herrmann et al, 2014) and cytochrome b (cytb) gene (e.g.…”

Section: Mitochondrial Locimentioning

confidence: 99%

Molecular approaches to trematode systematics: ‘best practice’ and implications for future study

et al. 2016

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'Molecular approaches to trematode systematics: 'best practice' and implications for future study ', Systematic Parasitology, 93(3), 295-306. The final publication is available at Springer via http://dx.doi.org/10.1007/s11230-016-9631-2.The full details of the published version of the article are as follows: However, since the late-1980s we have seen an increased integration of genetic data to overcome problems encountered when morphological data are considered in isolation. Here, we provide advice regarding the 'best molecular practice' for trematode taxonomy and systematic studies, in an attempt to help unify the field and provide a solid foundation to underpin future work.Emphasis is placed on defining the study goals and recommendations are made regarding sample preservation, extraction methods, and the submission of molecular vouchers. We advocate generating sequence data from all parasite species/host species/geographic location combinations and stress the importance of selecting two independently evolving loci (one ribosomal and one mitochondrial marker). We recommend that loci should be chosen to provide genetic variation suitable to address the question at hand and for which sufficient 'useful' comparative sequence data already exist. Quality control of the molecular data via using proof-reading Taq polymerase, sequencing PCR amplicons using both forward and reverse primers, ensuring that a minimum of 85% overlap exists when constructing consensus sequences, and checking electropherograms by eye is stressed. We advise that all genetic results are best interpreted using a holistic biological approach, which considers morphology, host identity, collection locality, and ecology. Finally, we consider what advances next-generation sequencing holds for trematode taxonomy and systematics.

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Revealing the secret lives of cryptic species: Examining the phylogenetic relationships of echinostome parasites in North America

Cited by 123 publications

References 35 publications

Metagonimoides oregonensis (Heterophyidae: Digenea) Infection In Pleurocerid Snails and Desmognathus quadramaculatus Salamander Larvae In Southern Appalachian Streams

Metagonimoides oregonensis (Heterophyidae: Digenea) Infection In Pleurocerid Snails and Desmognathus quadramaculatus Salamander Larvae In Southern Appalachian Streams

Evolved pesticide tolerance influences susceptibility to parasites in amphibians

Molecular approaches to trematode systematics: ‘best practice’ and implications for future study

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