Revealment study on the regulation of lipid metabolism by Lingguizhugan Decoction in heart failure treatment based on integrated lipidomics and proteomics

Wang, Xu; Gao, Yanhua; Zhang, Jia; Zhang, Huaxing; Sun, Shuo; Su, Suwen; Kong, Dezhi; Wang, Qiao

doi:10.1016/j.biopha.2022.114066

Cited by 7 publications

(3 citation statements)

References 56 publications

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“…Adipocytes involve three neutral lipases in TG breakdown, with adipose triglyceride lipase (ATGL; encoded by PNPLA2) being the primary enzyme responsible for TG hydrolysis to DAG. Hormone-sensitive lipase (HSL; encoded by LIPE) serves as the second enzyme, hydrolyzing DAG and displaying TAG hydrolysis activity. − Resveratrol-induced activation of SIRT1 is facilitated through the phosphorylation of AMPK-α, and SIRT1 assumes a pivotal role in augmenting the process of fat browning within WAT by deacetylating PPARγ at Lys268 and Lys293. Furthermore, the central transcription factor PRDM16, activated by SIRT1, exerts influence over the modulation of transcription factors such as PGC-1α, thereby fostering the differentiation of white adipose tissue into brown adipocytes.…”

Section: Resultsmentioning

confidence: 99%

Development of Recombinant High-Density Lipoprotein Platform with Innate Adipose Tissue-Targeting Abilities for Regional Fat Reduction

Han,

Chen,

et al. 2024

ACS Nano

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As an escalating public health issue, obesity and overweight conditions are predispositions to various diseases and are exacerbated by concurrent chronic inflammation. Nonetheless, extant antiobesity pharmaceuticals (quercetin, capsaicin, catecholamine, etc.) manifest constrained efficacy alongside systemic toxic effects. Effective therapeutic approaches that selectively target adipose tissue, thereby enhancing local energy expenditure, surmounting the limitations of prevailing antiobesity modalities are highly expected. In this context, we developed a temperature-sensitive hydrogel loaded with recombinant high-density lipoprotein (rHDL) to achieve targeted delivery of resveratrol, an adipose browning activator, to adipose tissue. rHDL exhibits self-regulation on fat cell metabolism and demonstrates natural targeting toward scavenger receptor class B type I (SR-BI), which is highly expressed by fat cells, thereby achieving a synergistic effect for the treatment of obesity. Additionally, the dispersion of rHDL@Res in temperature-sensitive hydrogels, coupled with the regulation of their degradation and drug release rate, facilitated sustainable drug release at local adipose tissues over an extended period. Following 24 days' treatment regimen, obese mice exhibited improved metabolic status, resulting in a reduction of 68.2% of their inguinal white adipose tissue (ingWAT). Specifically, rHDL@Res/gel facilitated the conversion of fatty acids to phospholipids (PA, PC), expediting fat mobilization, mitigating triglyceride accumulation, and therefore facilitating adipose tissue reduction. Furthermore, rHDL@Res/gel demonstrated efficacy in attenuating obesity-induced inflammation and fostering angiogenesis in ingWAT. Collectively, this engineered local fat reduction platform demonstrated heightened effectiveness and safety through simultaneously targeting adipocytes, promoting WAT browning, regulating lipid metabolism, and controlling inflammation, showing promise for adipose-targeted therapy.

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Section: Resultsmentioning

confidence: 99%

Development of Recombinant High-Density Lipoprotein Platform with Innate Adipose Tissue-Targeting Abilities for Regional Fat Reduction

Han,

Chen,

et al. 2024

ACS Nano

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“…At present, LGZGD is commonly used to treat chronic heart failure, coronary heart disease, angina pectoris, allergic rhinitis, hypertension, chronic bronchitis, and Alzheimer's disease (AD) [5][6][7][8][9][10]. For example, Xi et al confirmed the inhibitive effects of LGZGD on the overexpression of proinflammatory cytokines IL-1β, IL-6, TNF-α, and NO by activated microglia [11].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Qualitative and quantitative studies on chemical constituents of Ling‐gui‐zhu‐gan decoction: In vitro and in vivo

Gao

Wang

et al. 2023

J of Separation Science

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Ling‐gui‐zhu‐gan decoction has significant therapeutic effects in the treatment of diseases related to phlegm and fluid retention. In this study, we aimed to qualitatively characterize the chemical constituents of Ling‐gui‐zhu‐gan decoction in vitro and in vivo by HPLC coupled to Fourier transform ion cyclotron resonance MS, and quantitively determine the contents of typical chemical constituents by HPLC method. As a result, a total of 75 chemical constituents were discovered including 37 flavonoids and their glycosides, 20 saponins, 9 sterols, 3 organic acids and their derivatives, 3 lactones, 2 coumarins, and 1 alcohol. Among them, 17 chemical constituents were specifically identified. Subsequently, an HPLC method was established for simultaneous determination of seven chemical constituents. Finally, a total of 40 prototype components were initially detected by HPLC‐MS method in the biological samples of rats after their water extract was orally administrated. Among them, 29, 27, 12, and 32 prototype components were detected in plasma, bile, urine, and feces, respectively. Moreover, 34 metabolites, including 16 phase II metabolites, were detected for the first time. In conclusion, this study lays the foundation for the identification of chemical components in vitro and in vivo and the elucidation of the potential pharmacodynamic components of Ling‐gui‐zhu‐gan decoction.

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Study on the mechanism of Xinmailong injection against chronic heart failure based on transcriptomics and proteomics

Xue,

Lv,

Jin

et al. 2025

Journal of Pharmaceutical and Biomedical Analysis

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Revealment study on the regulation of lipid metabolism by Lingguizhugan Decoction in heart failure treatment based on integrated lipidomics and proteomics

Cited by 7 publications

References 56 publications

Development of Recombinant High-Density Lipoprotein Platform with Innate Adipose Tissue-Targeting Abilities for Regional Fat Reduction

Development of Recombinant High-Density Lipoprotein Platform with Innate Adipose Tissue-Targeting Abilities for Regional Fat Reduction

Qualitative and quantitative studies on chemical constituents of Ling‐gui‐zhu‐gan decoction: In vitro and in vivo

Study on the mechanism of Xinmailong injection against chronic heart failure based on transcriptomics and proteomics

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