Revelation of fibroblast protein commonalities and differences and their possible roles in wound healing and tumourigenesis using co‐culture models of cells

Jarkovská, Karla; Dvořánková, Barbora; Halada, Petr; Kodet, Ondřej; Szabó, Pavol; Gadher, Suresh Jivan; Motlı́k, Jan; Kovářová, Hana; Smetana, Karel

doi:10.1111/boc.201400014

Cited by 11 publications

(6 citation statements)

References 35 publications

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“…In both cases, these stimulated fibroblasts significantly elevated expression of keratin 8 in normal human keratinocytes [ 56 ]. These in vitro activated fibroblasts are also remarkably similar to CAFs as demonstrated by proteomic approach [ 94 ]. Interestingly, this stimulation of normal dermal fibroblasts in response to presence of normal/malignant keratinocytes is only transient.…”

Section: Cafs As the Master Cell Type In Tumor Stromamentioning

confidence: 84%

Cancer Microenvironment: What Can We Learn from the Stem Cell Niche

Lacina

Plzák

Kodet

et al. 2015

IJMS

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Epidermal stem cells (ESCs) are crucial for maintenance and self- renewal of skin epithelium and also for regular hair cycling. Their role in wound healing is also indispensable. ESCs reside in a defined outer root sheath portion of hair follicle—also known as the bulge region. ECS are also found between basal cells of the interfollicular epidermis or mucous membranes. The non-epithelial elements such as mesenchymal stem cell-like elements of dermis or surrounding adipose tissue can also contribute to this niche formation. Cancer stem cells (CSCs) participate in formation of common epithelial malignant diseases such as basal cell or squamous cell carcinoma. In this review article, we focus on the role of cancer microenvironment with emphasis on the effect of cancer-associated fibroblasts (CAFs). This model reflects various biological aspects of interaction between cancer cell and CAFs with multiple parallels to interaction of normal epidermal stem cells and their niche. The complexity of intercellular interactions within tumor stroma is depicted on example of malignant melanoma, where keratinocytes also contribute the microenvironmental landscape during early phase of tumor progression. Interactions seen in normal bulge region can therefore be an important source of information for proper understanding to melanoma. The therapeutic consequences of targeting of microenvironment in anticancer therapy and for improved wound healing are included to article.

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Section: Cafs As the Master Cell Type In Tumor Stromamentioning

confidence: 84%

Cancer Microenvironment: What Can We Learn from the Stem Cell Niche

Lacina

Plzák

Kodet

et al. 2015

IJMS

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“…Cytotoxicity of the NaGdF 4 :Yb 3+ /Er 3+ and NaGdF 4 :Yb 3+ /Er 3+ @PEG nanoparticles was evaluated on a cell line derived from human cervical carcinoma (HeLa) and human dermal fibroblast (HF) 46 – 48 kindly provided by Dr. Mělková and Dr. Dvořánková, respectively, First Faculty of Medicine, Charles University, Prague. 5∙10 3 of HeLa or 8∙10 3 of HF cells were seeded in 100 µl of media into 96-well flat-bottom TPP plates (Merck; Darmstadt, Germany) for 24 h. Subsequently, the nanoparticles (0.4–400 µg/ml) were added, the cells were cultivated at 37 °C for 72 h under 5% CO 2 atmosphere, AlamarBlue cell viability reagent (10 µl) was added to each well, and the incubation continued at 37 °C for 4 h. The active component of the AlamarBlue reagent (resazurin) was reduced to the highly fluorescent resorufin only in viable cells.…”

Section: Methodsmentioning

confidence: 99%

Highly colloidally stable trimodal 125I-radiolabeled PEG-neridronate-coated upconversion/magnetic bioimaging nanoprobes

Kostiv

Kučka

Lobaz

et al. 2020

Sci Rep

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Abstract“All-in-one” multifunctional nanomaterials, which can be visualized simultaneously by several imaging techniques, are required for the efficient diagnosis and treatment of many serious diseases. This report addresses the design and synthesis of upconversion magnetic NaGdF4:Yb3+/Er3+(Tm3+) nanoparticles by an oleic acid-stabilized high-temperature coprecipitation of lanthanide precursors in octadec-1-ene. The nanoparticles, which emit visible or UV light under near-infrared (NIR) irradiation, were modified by in-house synthesized PEG-neridronate to facilitate their dispersibility and colloidal stability in water and bioanalytically relevant phosphate buffered saline (PBS). The cytotoxicity of the nanoparticles was determined using HeLa cells and human fibroblasts (HF). Subsequently, the particles were modified by Bolton-Hunter-neridronate and radiolabeled by 125I to monitor their biodistribution in mice using single-photon emission computed tomography (SPECT). The upconversion and the paramagnetic properties of the NaGdF4:Yb3+/Er3+(Tm3+)@PEG nanoparticles were evaluated by photoluminescence, magnetic resonance (MR) relaxometry, and magnetic resonance imaging (MRI) with 1 T and 4.7 T preclinical scanners. MRI data were obtained on phantoms with different particle concentrations and during pilot long-time in vivo observations of a mouse model. The biological and physicochemical properties of the NaGdF4:Yb3+/Er3+(Tm3+)@PEG nanoparticles make them promising as a trimodal optical/MRI/SPECT bioimaging and theranostic nanoprobe for experimental medicine.

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“…Overexpression of CALDESMON, associated with Actin and HSP70, has been previously described in the setting of tissue injury. 49 …”

Section: Discussionmentioning

confidence: 99%

Topical heparin as an effective and safe treatment for patients with capecitabine-induced hand-foot syndrome: results of a phase IIA trial supported by proteomic profiling of skin biopsies

et al. 2022

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Background: Hand-foot syndrome (HFS) is a common adverse reaction associated with capecitabine chemotherapy that significantly affects the quality of life of patients. This study evaluates the safety and effectiveness of a topical heparin (TH) treatment on the clinical manifestations and anatomopathological alterations of capecitabine-induced HFS. In addition, we performed proteome profiling of skin biopsies obtained from patients with HFS at baseline and after heparin treatment. Methods: Patients with grade ⩽ 2 HFS associated with capecitabine were included in this study. The primary end point was the effectiveness of TH in reducing HFS of any grade. Clinical improvement was evaluated by clinicians, and an improvement was perceived by patients who performed a weekly visual analog scale questionnaire. Secondary end points included a comparative histological analysis and protein expression in skin biopsies at baseline and after 3 weeks of HT treatment. Proteomic profiling was carried out using quantitative isobaric labelling and subsequently validated by a T-array. Results: Twenty-one patients were included in the study. The median TH treatment time was 7.6 weeks (range = 3.6–41.6 weeks), and the median response time was 3.01 weeks (95% CI = 2.15–3.97). At the end of treatment, 19 of 21 patients (90.48%) responded to treatment with a decrease in one or more grades of HFS. None of the patients experienced adverse effects related to TH usage, nor did they suspend chemotherapy treatment. The main findings observed in skin biopsies after treatment were a decrease in hyperkeratosis and lymphocytic infiltrates. The proteomic analysis showed altered expression of 34 proteins that were mainly related to wound healing, cell growth, and the immune response. Conclusion: Based on our results, topical heparin is an effective and safe treatment for clinical manifestations of HFS, probably due to the restauration of skin homeostasis after heparin treatment, as supported by our proteomics-derived data. Trial registration: EudraCT 2009-018171-13

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Revelation of fibroblast protein commonalities and differences and their possible roles in wound healing and tumourigenesis using co‐culture models of cells

Abstract: The findings provide strong support for crucial role of stromal microenvironment in wound healing and tumourigenesis. In particular, epithelia-induced protein changes in fibroblasts offer new potential targets which may lead to novel tailored cancer therapeutic strategies.

Cited by 11 publications

References 35 publications

Cancer Microenvironment: What Can We Learn from the Stem Cell Niche

Cancer Microenvironment: What Can We Learn from the Stem Cell Niche

Highly colloidally stable trimodal 125I-radiolabeled PEG-neridronate-coated upconversion/magnetic bioimaging nanoprobes

Topical heparin as an effective and safe treatment for patients with capecitabine-induced hand-foot syndrome: results of a phase IIA trial supported by proteomic profiling of skin biopsies

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