Revelation of β-sitosterol from Benincasa hispida Seeds, Carissa congesta Roots and Polyalthia longifolia Leaves by High Performance Liquid Chromatography

Abstract

Background: Fruit juice of Benincasa hispida (BH) is regarded as Vrindamadhava that is recommended for internal use in snake's bites. The fruits have been reported to contain good amount of proteins, enzymes, Vitamin B 1 and C, flavonoid C-glycoside, terpenes, phenolic acids and free sugars such as glucose, rhamnose, mannitol, uronic acid, astilbin, catechin, naringenin, pectic polysaccharides and even some trace metals. Polyalthia longifolia (PL) has been well known for its ayurvedic preparations such as Sitaphala kula, Kaphapitashama, Anulomak and Krimighna. PL leaves has been reported to contain phytoconstituents like polylongine, Aporphine-Noxide alkaloids, allo-aromadendrene, caryophyllene oxide, β-caryophyllene, β-selinene, α-humulene, α-pinene and camphene. Carissa congesta (CC), known as Bengal currant, have yielded 2-acetyl phenol, carissone, carindone and Des-n-methylnoracronycine. β-sitosterol, an important phytoconstituent recognized from all these selected plants has been well known for its ethnopharmacological importance. In this new study, the research team members has focused on determining the percentage of the β-sitosterol present in the BH seeds, CC roots and PL leaves by subjecting the ex-

Quantitative Estimation of Immunomodulatory Flavonoid Quercetin by HPTLC in Different Leafy Vegetables Available in West Bengal

“…150 L/s was applied at a continuous rate. [24] Toluene-Ethyl acetate-Formic acid 5:4:0.2 (% v/v/v) [25] was used as the mobile phase to carry out linear ascending development of the plates to a distance of 80 mm in a CAMAG manual Developing Chamber 2 that had been previously saturated with the mobile phase developed for 30 min at 22°C.…”