Reversal effect of adenovirus-mediated human interleukin 24 transfection on the cisplatin resistance of A549/DDP lung cancer cells

Abstract

Interleukin-24 (IL-24) is a tumor-suppressor gene that has been documented in human melanoma cells. IL-24 has marked antitumor activities on various types of human cancer, but its underlying mechanism remains unclear. In the present, we investigated the effects of human IL-24 (hIL-24) on the chemotherapy resistance of lung cancer cells. The cisplatin (DDP)-resistant lung carcinoma cell line A549/DDP was subjected to adenovirus-mediated transfection with the human IL-24 gene (Ad-hIL-24). The growth-inhibitory and apoptotic effects of Ad-hIL-24 on A549/DDP cells were observed, and the expression levels of AKT, phosphorylated-AKT (p-AKT) and P-glycoprotein (P-gp) were detected. Ad-hIL-24 significantly decreased the levels of p-AKT and P-gp, and effectively inhibited A549/DDP cell growth. Furthermore, A549/DDP cells exhibited a significantly increased rate of apoptosis, as well as G2/M-phase arrest, following transfection with Ad-hIL-24, and these effects were increased in cells treated with Ad-IL-24 combined with DDP when compared with those treated with Ad-hIL-24 or DDP alone. These results suggest that hIL-24 can reverse the DDP resistance of lung cancer cells, and that the associated mechanism involves the induction of apoptosis and G2/M-phase arrest through the phosphoinositide3-kinase (PI3K)/AKT signaling pathway, as well as a decrease in drug resistance through P-gp expression.

RETRACTED ARTICLE: MNAT1 is overexpressed in colorectal cancer and mediates p53 ubiquitin-degradation to promote colorectal cancer malignance

“…Apoptosis analysis was performed as described previously [ 32 ]. Briefly, 1 × 10 4 cells of shscramble-HCT116, shMNAT1 # 1-HCT116, shMNAT1 # 2-HCT116, pSIN-HEK293T, and pSIN-MNAT1-HEK293T were seeded on six-well plates and cultured to reach 70% confluence, and were treated with 10 or 80 μg/ml 5-fluorouracil (5-FU).…”

Adenovirus-Mediated LAMA3 Transduction Enhances Hemidesmosome Formation and Periodontal Reattachment during Wound Healing

“…29,30 In the current study, regarding GFP expression, adenoviral vectors have high transduction efficiency, which reduces the dose of the viral vector and increases therapeutic safety, consistent with the findings of previous studies. 31,32 To make a biological assessment of Ad-LAMA3 in vitro, we selected HaCaT cells. First, the viability of HaCaT cells for Ad-LAMA3 treatment was evaluated.…”

Ad-VT enhances the sensitivity of chemotherapy-resistant lung adenocarcinoma cells to gemcitabine and paclitaxel in vitro and in vivo

“…High and persistent expression of P-gp increases the resistance of the lung squamous cell carcinoma cell line CH27 to doxorubicin [22]. At present, many drugs have been developed and applied to prevent or mitigate chemotherapy resistance in lung cancer, including calcium channel blockers, antiarrhythmic drugs and some components of traditional Chinese medicine [23]. Although there are drugs that can temporarily increase the concentration of chemotherapeutic drugs to sufficient levels that will kill tumor cells, they cannot be widely used in the clinic due to their instability, nonspecificity and adverse effects.…”