Reversal of multidrug resistance in cancer cells by Rhizoma Alismatis extract

Fong, Wang‐Fun; Wang, C.; Zhu, Guo‐Yuan; Leung, Chung‐Hang; Yang, Mengsu; Cheung, Hon-Yeung

doi:10.1016/j.phymed.2006.03.004

Cited by 53 publications

(29 citation statements)

References 12 publications

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“…Fong et al have reported that Alismatis Rhizoma extract showed a synergistic growth inhibitory effect with cancer drugs that are MDR-1 substrates and prevented the efflux of rhodamin-123 from doxorubicin-resistant cancer cells. 17) Taken together, these results suggest that Alismatis Rhizoma overcomes multidrug-resistance by suppressing the drug-efflux function of MDR-1, and the reversal effects of both takushato and goreisan to multidrug resistance are derived from Alismatis Rhizoma. On the other hand, Atractylodis Rhizoma, a component of both takushato and goreisan, prevented the export of calcein-AM by MDR-1, although the combined effect of Atractylodis Rhizoma and paclitaxel on HuH-7/PTX cell growth was not observed, indicating that Atractylodis Rhizoma is not involved in the reversal effects of both takushato and goreisan to multidrug resistance.…”

Section: Discussionmentioning

confidence: 81%

Effects of Kampo Medicines on MDR-1-Mediated Multidrug Resistance in Human Hepatocellular Carcinoma HuH-7/PTX Cells

Hyuga

Shiraishi

Hori

et al. 2012

Biological & Pharmaceutical Bulletin

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Paclitaxel-resistant HuH-7 (HuH-7/PTX) cells were established by one-week exposure of HuH-7 cells to paclitaxel to analyze the effects of Kampo medicines on MDR-1-mediated multidrug resistance. HuH-7/PTX cells expressed high levels of MDR-1 and efficiently exported calcein-acetoxymethylester (calcein-AM), which is a substrate of MDR-1, suggesting that HuH-7/PTX cells resist paclitaxel by overexpressing MDR-1. We assessed the effects of 26 kinds of Kampo medicine on MDR-1 by calcein-AM efflux assay using HuH-7/PTX cells, and the results revealed that takushato and goreisan are potential inhibitors of drug efflux by MDR-1. Additionally, the sensitivity of HuH-7/PTX cells to paclitaxel was increased in combination with these Kampo medicines, indicating that takushato and goreisan overcame paclitaxel resistance in the cells by suppressing drug export by MDR-1. We further clarified that Alismatis Rhizoma contained in both takushato and goreisan reversed paclitaxel resistance by preventing drug efflux by MDR-1 without affecting the expression levels of MDR-1. Moreover, the principal components of Alismatis Rhizoma, Alisol A, Alisol B, and Alisol B acetate, were found to increase the sensitivity to paclitaxel in HuH-7/PTX by inhibiting drug export by MDR-1 without affecting the expression levels of MDR-1. These results suggested that the reversal effects of takushato and goreisan on paclitaxel resistance are derived from these principal components in Alismatis Rhizoma. Accordingly, Kampo medicines containing Alismatis Rhizoma such as takushato and goreisan may be useful as MDR-1 inhibitors.

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Section: Discussionmentioning

confidence: 81%

Effects of Kampo Medicines on MDR-1-Mediated Multidrug Resistance in Human Hepatocellular Carcinoma HuH-7/PTX Cells

Hyuga

Shiraishi

Hori

et al. 2012

Biological & Pharmaceutical Bulletin

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“…EtOH extract of Goldfussia psilostachys 0.5 μg/ml ↟ CML cells in G 2 /M phase [252] Fraction Alisma orientalis (Sam) Juzep extractReverse of MDR [265] Polyphenolic extract of Ichnocarpus fructescens leaves -At 5, 10, 20 μg/ml con. ↓K562 cell proliferation [266] EtOH extract of Orbignya speciosa 33.9 ± 4.3 μg/ml -…”

Section: Others Natural Productsmentioning

confidence: 99%

Natural Products for Treatment of Chronic Myeloid Leukemia

Khajapeer¹,

Baskaran²

2016

Anti-Cancer Drugs - Nature, Synthesis and Cell

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Chronic myeloid leukemia (CML) is a hematological malignancy that arises due to reciprocal translocation of 3′ sequences from c-Abelson (abl) protooncogene on chromosome 9 with 5′ sequence of truncated break point cluster region (bcr) to chromosome 22. The fusion gene product BCR-ABL, a functional oncoprotein p210, is a constitutively activated tyrosine kinase that activates several cell proliferative signaling pathways. BCR-ABL-specific tyrosine kinase inhibitors (TKIs) such as imatinib, nilotinib and ponatinib potently inhibit CML progression. However, drug resistance owing to BCR-ABL mutations and overexpression is still an issue. Natural products are chemical compounds or substances produced by living organisms. They are becoming an important research area for cancer drug discovery due to their low toxicity and cost-effectiveness. Several lines of evidence show that many NPs such as alkaloids, flavonoids, terpenoids, polyketides, lignans and saponins inhibit CML cell proliferation and induce apoptosis. NPs not only differentiate CML cells into monocyte/erythroid lineage but also can reverse the multi-drug resistance (MDR) in CML cells. In this chapter, we review the anti-CML activity of various NPs.

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“…Inhibition of P-gp [55] In vitro Induction of P-gp expression [56] Coptis chinensis Inflammatory symptoms…”

Section: In Vitromentioning

confidence: 99%

The effect of herbal materials on the p-glycoprotein activity and function

et al. 2013

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S u m m a r y P-glycoprotein (P-gp) encoded by the MDR1 (multidrug resistance 1) gene is ATP-dependent transporting protein which is localizated in the cell membrane. P-gp is expressed REVIEW ARTICLE Unauthenticated Download Date | 6/7/19 11:36 AM 130 A. Bogacz, D. Deka-Pawlik, J. Bartkowiak-Wieczorek, M. Karasiewicz, R. Kujawski, A. Kowalska, A. Chałas, B. Czerny, E.Grześkowiak, P. M. Mrozikiewicz mainly in organs with the secretory functions and its physiological role concerns tissue protection against xenobiotics. P-glycoprotein is involved in the permeability barriers of the blood-brain, blood-placenta directly protecting these organs. It participates in the transport of many drugs and other xenobiotics affecting their absorption, distribution, metabolism and excretion. The high P-gp activity in the cell membranes of cancer tissue is a major cause of lack of effectiveness of chemotherapy. Hence, the methods which could increase the sensibility of these pathological cells to cytostatics are still being searched.In the experimental studies it was shown that natural plant substances may have an effect on the expression level and activity of P-glycoprotein. Hypericum perforatum, ginkgo biloba and Camellia sinensis increase P-gp activity while curcumin from Curcuma longa, piperine and silymarin inhibit this protein. Taking into account a wide substrate spectrum of P-gp, application of our knowledge on interactions of herbals and synthetic drugs should be considered in order to improve drug impact on different tissues.

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Reversal of multidrug resistance in cancer cells by Rhizoma Alismatis extract

Cited by 53 publications

References 12 publications

Effects of Kampo Medicines on MDR-1-Mediated Multidrug Resistance in Human Hepatocellular Carcinoma HuH-7/PTX Cells

Effects of Kampo Medicines on MDR-1-Mediated Multidrug Resistance in Human Hepatocellular Carcinoma HuH-7/PTX Cells

Natural Products for Treatment of Chronic Myeloid Leukemia

The effect of herbal materials on the p-glycoprotein activity and function

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