Reverse ELISA for IgG and IgM antibodies to detect Lassa virus infections in Africa

Specific detection of antibodies to different flaviviruses using a new immune complex ELISA

“…2) Here, two diVerent test procedures were compared: on one strip (designated with anti-IgG), the serum and TBE-ELA were applied simultaneously as usual, while on a parallel strip after an incubation of the serum samples for 1 h, the serum was washed away (Anti-IgG wash) and subsequently the TBE-ELA was applied over night. Interestingly, the reaction of the ++ serum was clearly stronger without washing away unbound IgG antibody compared to the procedure with additional washing (P/N ratio of the ++ sample: 6 and 4, respectively).…”

“…In various publications, we had described highly sensitive IC ELISAs for the detection of IgG antibodies to cytomegalovirus [1], Lassa virus [2] and to type-speciWc antigens on the surface of West Nile (WN)-and tick-borne encephalitis (TBE) viruses [3,4]. The latter two tests, using the ED3 domain of the respective Xaviviruses, did no longer show the well-known cross-reactivity among Xaviviruses, frequently seen using commercially available routine ELISAs or indirect immunoXuorescence (IIF).…”

Laboratory Diagnosis of Lassa Fever

“…Multiple antigen and IgM capture ELISAs have been developed using inactivated virus (36,(52)(53)(54); however this approach is limited to BSL-4 capable facilities. The use of recombinant antigens allows for improved assay development and access (50, 55-57).…”

“…Historically, antibody detection was conducted using immunofluorescence assays (IFAs) (4,13,36); however, IFAs have been replaced over time with ELISAs due to their ease of use, their increased sensitivity and specificity, and reduced interobserver variation in readings (36,54). Estimates of the sensitivity of IgM detection for diagnosing Lassa fever compared with that of RT-PCR range from 55% to 72% (36,61,62).…”

A Multi-Faceted Diagnostic Approach to Lung Infections in Patients with Cystic Fibrosis

“…The develop color is read by spectrophotometer (Sakaguchi et al, 1989). The ogives can be sensitized to the reagents and thus be able to detect more than one antigen at a time (Emmerich et al, 2006). In addition, sample sizes can be optimized to achieve high levels of antigen detection (Emmerich et al, 2006) The ELISA technique has been performed on numerous types of samples for numerous organisms including P. aeruginosa and A. fumigates in CF sera (Brett et al, 1988;Knutsen et al, 1994).…”