Reverse-Genetic Analysis of the Two Biotin-Containing Subunit Genes of the Heteromeric Acetyl-Coenzyme A Carboxylase in Arabidopsis Indicates a Unidirectional Functional Redundancy

Xu, Li; Ilarslan, Hilal; Brachova, Libuse; Qian, Hui-Rong; Li, Ling; Che, Ping; Wurtele, Eve Syrkin; Nikolau, Basil J.

doi:10.1104/pp.110.165910

Cited by 60 publications

(61 citation statements)

References 85 publications

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“…The BCCP1 transcript is expressed constitutively, while BCCP2 is predominant in flowers and siliques and its overall abundance is approximately 4-fold lower than that of BCCP1 (Thelen et al, 2000). The development of the endosperm and embryo is disrupted when BCCP1 is knocked-out during Arabidopsis seed development, and fatty acid accumulation decreases and normal vegetative plant growth is severely affected when BCCP1 accumulation is reduced at the protein level (Li et al, 2011). These results indicate that BCCP genes are involved in de novo lipid synthesis storage and accumulation (Elborough et al, 1996;Thelen et al, 2000;Gu et al, 2011).…”

Section: Introductionmentioning

confidence: 72%

“…The production of malonyl-CoA catalyzed by ACCase is the rate-limiting step in the process of fatty acid biosynthesis (Thelen et al, 2000;Fukuda et al, 2013). Previous studies have confirmed that heteromeric ACCase plays an important regulatory function during oil synthesis and accumulation in plant seeds (Thelen et al, 2000;Dong et al, 2004;Hu et al, 2009;Liu et al, 2009;Li et al, 2011). Homomeric ACCase is composed of a large multifunctional polypeptide (Sasaki and Nagano, 2004) and heteromeric ACCase is composed of a biotin carboxyl carrier protein (BCCP), biotin carboxylase (BC), and carboxyltransferase (CT), which consists of α-and β-subunits.…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and expression analysis of a novel BCCP subunit gene from Aleurites moluccana

Xuan¹,

Zhang²,

Liu³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Aleurites moluccana L. is grown as a roadside tree in southern China and the oil content of its seed is higher than other oil plants, such as Jatropha curcas and Camellia oleifera. A. moluccana is considered a promising energy plant because its seed oil could be used to produce biodiesel and bio-jet fuel. In addition, the bark, leaves, and kernels of A. moluccana have various medical and commercial uses. Here, a novel gene coding the biotin carboxyl carrier protein subunit (BCCP) was cloned from A. moluccana L. using the homology cloning method combined with rapid amplification of cDNA end (RACE) technology. The isolated full-length cDNA sequence (designated AMaccB) was 1188 bp, containing a 795-bp open reading frame coding for 265 amino acids. The deduced amino acid sequence of AM-accB contained a biotinylated domain located between amino acids 190 and 263. A. moluccana BCCP shows high identity at the amino acid level to its homologues in other higher plants, such as Vernicia fordii, J. curcas, and Ricinus communis (86, 77, and 70%, respectively), which all contain conserved domains for ACCase activity. The expression of the AM-accB gene during the middle stage of development and maturation in A. moluccana seeds was higher than that in early and later stages. The expression pattern of the AM-accB gene is very similar to that of the oil accumulation rate.

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Section: Introductionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Molecular cloning and expression analysis of a novel BCCP subunit gene from Aleurites moluccana

Xuan¹,

Zhang²,

Liu³

et al. 2015

Genet. Mol. Res.

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“…BCCP1 is an essential subunit for a heteromeric acetylCoA carboxylase, which catalyzes the ATP-dependent formation of malonyl-CoA in the first committed step of the FA biosynthetic pathway (Ohlrogge and Browse, 1995). Down-regulation of BCCP1 decreases FA accumulation in seeds and severely affects normal vegetative plant growth (Li et al, 2011). The down-regulation of KAR5 and BCCP1 expression in siliques (2 DAF) may explain the decreased FA accumulation in tt16 RNAi transgenic plants.…”

Section: Discussionmentioning

confidence: 99%

Transparent Testa16Plays Multiple Roles in Plant Development and Is Involved in Lipid Synthesis and Embryo Development in Canola

et al. 2012

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Transparent Testa16 (TT16), a transcript regulator belonging to the B sister MADS box proteins, regulates proper endothelial differentiation and proanthocyanidin accumulation in the seed coat. Our understanding of its other physiological roles, however, is limited. In this study, the physiological and developmental roles of TT16 in an important oil crop, canola (Brassica napus), were dissected by a loss-of-function approach. RNA interference (RNAi)-mediated down-regulation of tt16 in canola caused dwarf phenotypes with a decrease in the number of inflorescences, flowers, siliques, and seeds. Fluorescence microscopy revealed that tt16 deficiency affects pollen tube guidance, resulting in reduced fertility and negatively impacting embryo and seed development. Moreover, Bntt16 RNAi plants had reduced oil content and altered fatty acid composition. Transmission electron microscopy showed that the seeds of the RNAi plants had fewer oil bodies than the nontransgenic plants. In addition, tt16 RNAi transgenic lines were more sensitive to auxin. Further analysis by microarray showed that tt16 downregulation alters the expression of genes involved in gynoecium and embryo development, lipid metabolism, auxin transport, and signal transduction. The broad regulatory function of TT16 at the transcriptional level may explain the altered phenotypes observed in the transgenic lines. Overall, the results uncovered important biological roles of TT16 in plant development, especially in fatty acid synthesis and embryo development.The plant MADS box family genes, which are named after the characterization of four members in this group, MINICHROMOSOME MAINTENANCE1, AGAMOUS, DEFICIENS and SERUM RESPONSE FACTOR, encode transcription factors that share a common DNA-binding domain (the MADS box) and play multiple roles in flower pattern formation, gametophyte cell division, and fruit wall differentiation (Ng and Yanofsky, 2001;Dinneny and Yanofsky, 2005;Colombo et al., 2008).A number of MADS domain proteins from vascular plants share a conserved structural organization, the so-called MIKC-type domain structure, where the MADS (M) domain is followed by an Intervening (I), a Keratin-like (K) and a C-terminal domain (Theissen et al., 1996). MIKC-type MADS box genes are involved in important aspects of plant reproductive development, such as flower initiation, specification of floral meristem and organ identity, and ovule and fruit development (Becker and Theissen, 2003).MADS box genes are the major members of plant floral organ identity genes, which have been divided into five classes according to the ABCDE model (Theissen, 2001;Krizek and Fletcher, 2005). The ABCDE model explains flower formation by the interaction of five classes of homeotic genes (A-E), with A controlling sepal, A+B+E controlling petal, B+C+E controlling stamen, C+E controlling carpel, and D controlling ovule development (Theissen, 2001;Krizek and Fletcher, 2005). Therefore, MADS box genes appear to have a central role in flower development. The functions of floral organ ...

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“…The BCCP1 isoform is constitutively expressed, while BCCP2 is predominantly expressed in the seed under the control of the WRINKLED1 transcription factor (Thelen et al, 2001;Ruuska et al, 2002;. Of these two isoforms, BCCP2 was shown to be more active in vitro (Thelen et al, 2001) but nonessential to plant growth (Li et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

A Family of Negative Regulators Targets the Committed Step of de Novo Fatty Acid Biosynthesis

et al. 2016

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ORCID ID: 0000-0002-9686-9645 (V.L.)Acetyl-CoA carboxylase (ACCase) catalyzes the committed step of de novo fatty acid biosynthesis. In prokaryotes, green algae, and most plants, this enzyme is a heteromeric complex requiring four different subunits for activity. The plant complex is recalcitrant to conventional purification schemes and hence the structure and composition of the full assembly have been unclear. In vivo coimmunoprecipitation using subunit-specific antibodies identified a novel family of proteins in Arabidopsis thaliana annotated as biotin/lipoyl attachment domain containing (BADC) proteins. Results from yeast two-hybrid and coexpression in Escherichia coli confirmed that all three BADC isoforms interact with the two biotin carboxyl carrier protein (BCCP) isoforms of Arabidopsis ACCase. These proteins resemble BCCP subunits but are not biotinylated due to a mutated biotinylation motif. We demonstrate that BADC proteins significantly inhibit ACCase activity in both E. coli and Arabidopsis. Targeted gene silencing of BADC isoform 1 in Arabidopsis significantly increased seed oil content when normalized to either mass or individual seed. We conclude the BADC proteins are ancestral BCCPs that gained a new function as negative regulators of ACCase after initial loss of the biotinylation motif. A functional model is proposed.

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Reverse-Genetic Analysis of the Two Biotin-Containing Subunit Genes of the Heteromeric Acetyl-Coenzyme A Carboxylase in Arabidopsis Indicates a Unidirectional Functional Redundancy

Cited by 60 publications

References 85 publications

Molecular cloning and expression analysis of a novel BCCP subunit gene from Aleurites moluccana

Molecular cloning and expression analysis of a novel BCCP subunit gene from Aleurites moluccana

Transparent Testa16Plays Multiple Roles in Plant Development and Is Involved in Lipid Synthesis and Embryo Development in Canola

A Family of Negative Regulators Targets the Committed Step of de Novo Fatty Acid Biosynthesis

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