Reverse-phase high-performance liquid chromatography/nuclear magnetic resonance spectrometry separations of biomolecules with 1-1 hard pulse solvent-suppression

Laude, David A.; Lee, R. W. K.; Wilkins, Charles L.

doi:10.1021/ac00284a064

Cited by 33 publications

(18 citation statements)

References 32 publications

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“…42 Solvent presaturation schemes for single detection coil continuous flow probes are unfavourable as presaturated nuclei are being continuously removed from the flow cell. Other methods for removing the solvent peak from the spectrum, in the case of flowing systems, 43 include selective excitation, binomial solvent suppression sequences 44 and post-acquisition solvent signal removal. [45][46][47] An alternative to using deuterated solvents, is the use of supercritical fluids (SFC), but this requires modification of the NMR probe.…”

Section: S/nmentioning

confidence: 99%

Process NMR spectrometry

Nordon

McGill

Littlejohn

2001

Analyst

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Section: S/nmentioning

confidence: 99%

Process NMR spectrometry

Nordon

McGill

Littlejohn

2001

Analyst

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“…By applying state-ofthe-art spectrometers (9.4 to 17.6 T; 400 to 750 MHz, respectively, for H observing frequence) a detection limit in the nanogram range can be achieved. The receiver has to be able to digitize a large dynamic range [9,10]. The dynamic range of the analog-to-digital converter (ADC) determines the ratio of the largest to the smallest NMR signal.…”

Section: Improvements Of the Spectrometermentioning

confidence: 99%

“…Ha et al [27] analyzed mixtures of three amino acids. Laude et al [10] as well as Laude and Wilkins [38] reported on the separation of some test mixtures (e.g. nucleosides, phenols).…”

Section: Other Applicationsmentioning

confidence: 99%

Hyphenation of high-performance liquid chromatography (HPLC) and other chromatographic techniques (SFC, GPC, GC, CE) with nuclear magnetic resonance (NMR): A review

Korhammer

Berndt

1996

Fresenius J Anal Chem

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The development of the hyphenation of highperformance liquid chromatography (HPLC) and other chromatographic methods (supercritical fluid chromatography (SFC), gel-permeation chromatography (GPC), gas chromatography (GC) and capillary electrophoresis (CE)) with nuclear magnetic resonance (NMR) is reviewed. The focus is not only on application (e.g. pharmaceutical chemistry, food chemistry, polymer analysis, fuel chemistry), but also on technical improvements (e.g. flow rate, flow cell, probe, solvent suppression). The increasing importance and overwhelming analytical possibilities of on-line coupling of HPLC with NMR is demonstrated. The literature up to March 1995 is considered.

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“…9 There are several publications using on-flow LC-NMR in various fields such as the confirmation and characterization of the chemical compositions of mixtures of aromatics, 5 identification of plant constituents, food, biomolecules, metabolomics and metabonomics. [10][11][12][13][14] Chemometrics or computational methods have been used for retention time measurement, 15 rank determination 16,17 and curve resolution [18][19][20] of LC-NMR data in recent publications. The most common procedures for curve resolution such as HELP, WFA and ALS do not have a good resolution in LC-NMR data.…”

Section: Introductionmentioning

confidence: 99%