Reversed-phase chromatographic behaviour of β-endorphin: Evidence of conformational change

Monger, L.; Olliff, Cedric J.

doi:10.1016/0021-9673(92)85153-k

Cited by 5 publications

(2 citation statements)

References 32 publications

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“…This regular helical structure has been implicated in binding with a TM helix of the opioid receptor 27. Evidence of conformational changes during gradient HPLC elution has also been presented 28. Particle beam‐LC/FTIR and ESI‐MS have recently been used to investigate conformations of BE in three different parenteral solutions 29.…”

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confidence: 98%

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Conformational changes in β‐endorphin as studied by electrospray ionization mass spectrometry

Lin¹,

Dass²

2001

Rapid Comm Mass Spectrometry

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Because of a wide range of physiological functions, the structure of beta-endorphin (BE) is of great interest. In this study, conformational changes in BE induced by methanol are explored with electrospray ionization-mass spectrometry (ESI-MS). Differences in the charge-state distribution (CSD) and the extent of hydrogen/deuterium (H/D) exchange were used to monitor the conformational changes. The latter experiments were conducted via time-resolved ESI-MS in a continuous-flow apparatus. Both these techniques demonstrate that BE exists in a random coil open structure in aqueous media, but it acquires a more compact conformation with increased concentration of methanol. The H/D exchange experiments reveal that BE forms 61% alpha-helix in mixed solvents.

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confidence: 98%

“…Previous work using other instrumental techniques has also provided evidence of secondary structure in BE 23–29. CD spectroscopy studies have shown that BE exists as a random coil in water, but it assumes as much as up to 50% α‐helical form or more in mixed solvent systems 23,.…”

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Conformational changes in β‐endorphin as studied by electrospray ionization mass spectrometry

Lin¹,

Dass²

2001

Rapid Comm Mass Spectrometry

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Gas and Liquid Chromatography, Column Selection for, in Drug Analysis

Duff

2000

Encyclopedia of Analytical Chemistry

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Within the realm of pharmaceutical drug analyses, two techniques currently dominate the field. High‐performance liquid chromatography (HPLC) is at the forefront, followed somewhat distantly by gas chromatography (GC). HPLC is more prevalent than GC owing mainly to the heat‐labile nature of the majority of drug substances. Still GC continues to play a very active role. In comparison to HPLC and GC, combined, the frequency of use of other techniques is quite small. Perhaps the major reason for this is that chromatography not only involves qualitative and quantitative detection of targeted species, but also provides a medium for isolating these analytes from potential interfering impurities prior to measurement. The proficiency of this combination is unparalleled by any other blending of means to clean up a sample and detect the target compound. These more prominent techniques are often complementary, although each has its own strengths and limitations. General guidelines are given to assist the chromatographer to elect HPLC and/or GC for a particular separation. Considerations for choosing hardware type and sizes, solid support and bonded phase are presented. Particular emphasis is placed on the latest, which is of primary importance for obtaining rugged methods. The reader is directed towards optimum phase selection based on the compound of interest's functional group type, or by its drug activity classification (e.g. barbiturate, cardiovascular, etc.). Assistance in developing new methods frequently arises when starting with an existing published method for a compound with similar structure. This article provides 514 references for more in‐depth separation information. In addition, background knowledge on bonded‐phase chemistry, chromatographic modes of separation, approaches to chromatographic chiral drug discrimination and processes for developing rugged methods is contributed.

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Separation of neuropeptide Y diastereomers by high-performance liquid chromatography and capillary zone electrophoresis

Kirby

Miller

Rivier

1993

Journal of Chromatography A

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Reversed-phase chromatographic behaviour of β-endorphin: Evidence of conformational change

Cited by 5 publications

References 32 publications

Conformational changes in β‐endorphin as studied by electrospray ionization mass spectrometry

Conformational changes in β‐endorphin as studied by electrospray ionization mass spectrometry

Gas and Liquid Chromatography, Column Selection for, in Drug Analysis

Separation of neuropeptide Y diastereomers by high-performance liquid chromatography and capillary zone electrophoresis

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