Reversed‐Phase High‐Performance Liquid Chromatographic Method for the Determination of Permaton Red (D&amp;C Red No. 36) in Cosmetics

Yoon, Juhee; Kim, Yejin; Kim, Kyu‐Bong; Park, Eun Ji; Na, Dong Hee

doi:10.1002/bkcs.11567

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…Precision and accuracy were determined using quality control (QC) samples containing cysteamine (2, 15, 150, and 500 μg/mL) and cystamine (5, 15, 150, and 500 μg/mL). Recovery was evaluated by comparing the peak areas of each 100 μg/mL cysteamine and cystamine spiked into blank cream prior to extraction to the peak areas of 100 μg/mL cysteamine and cystamine spiked into blank cream post-extraction (17,18).…”

Section: Methodsmentioning

confidence: 99%

Simultaneous Determination of Cysteamine and Cystamine in Cosmetics by Ion-Pairing Reversed-Phase High-Performance Liquid Chromatography

Kim

2019

ToxicolRes

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Cysteamine has been used in cosmetics as an antioxidant, a hair straightening agent, and a hair waving agent. However, recent studies indicate that cysteamine can act as an allergen to hairdressers. The objective of this study was to develop and validate a simple and effective reversed-phase high-performance liquid chromatography (RP-HPLC) method for the measurement of cysteamine and its dimer, cystamine. Sodium 1-heptanesulfonate (NaH-pSO) was used as an ion-pairing agent to improve chromatographic performance. Separation was performed on a Gemini C18 column (250 mm × 4.6 mm, 5 µm particle size) using a mobile phase composed of 85:15 (v/v) 4 mM NaHpSO in 0.1% phosphoric acid:acetonitrile. UV absorbance was monitored at 215 nm. The RP-HPLC method developed in this study was validated for specificity, linearity, limit of detection, limit of quantitation, precision, accuracy, and recovery. Cysteamine and cystamine were chromatographically resolved from other reducing agents such as thioglycolic acid and cysteine. Extraction using water and chloroform resulted in the recovery for cysteamine and cystamine ranging from 100.2-102.7% and 90.6-98.7%, respectively. This validated RP-HPLC method would be useful for quality control and monitoring of cysteamine and cystamine in cosmetics.

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Section: Methodsmentioning

confidence: 99%

Simultaneous Determination of Cysteamine and Cystamine in Cosmetics by Ion-Pairing Reversed-Phase High-Performance Liquid Chromatography

Kim

2019

ToxicolRes

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“…At the same time, the analysis of these assays with high-precision analyzers makes regular analysis challenging. For this purpose, various analytical methods such as thin layer chromatography, spectrophotometry, derivative spectrophotometry, fluorimetry, voltammetry, differential pulse polarography, capillary electrophoresis, HPLC and ion chromatography have been used [9,12,[31][32][33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

“…HPLC techniques are well suited for the analysis of samples containing several colorants. These techniques provide high sensitivity and resolution, and allow simultaneous determination of colorants from the sample [11,[33][34][35].…”

Section: Introductionmentioning

confidence: 99%

Determination of synthetic colorants in cosmetic products by reversed-phase high-performance liquid chromatography coupled with diode-array detector

Filiz¹,

Oymak²,

Dural³

2019

Sanat

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This study aimed at developing and validating a reversed-phase (RP) high-performance liquid chromatography (HPLC) method, for simultaneous determination of five synthetic dyes called tartrazine (TRZ), sunset yellow (SY), allura red AC (AR), brilliant blue FCF (BB) and erythrosine B (EB) in cosmetic samples. The separation was performed by a C18 reverse phase analytical column (4.6 x 250 mm, 5 µm) at 30°C with gradient elution and the mobile phase contained 20 mM ammonium acetate buffer, acetonitrile and methanol. Flow was 1.0 mL/min. Detection wavelengths of diode array detector (DAD) were set at 420, 480, 510, 634 and 530 nm for TRZ, SY, AR, BB and EB, respectively. The dyes were analysed in 24 min. The limits of detection (LOD) was ≤ 0.18 µg/mL. The recovery was between 88.7 and 103.0%. Precision was ≤ .7.33 (RSD%) and accuracy was ≤ 3.0 (RE%). It was determined that 7 different cosmetic samples analyzed, consisting of soap, shower gel, eyeshadow, mouthwash, and lip pencil contained synthetic dyes at a concentration of 0.29 to 10.81 mg/g.

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Determination of sunset yellow, allura red, and fast green using a novel magnetic nanoadsorbent modified with Elaeagnus angustifolia based on magnetic solid-phase extraction by HPLC

Oymak

Dural

2022

Braz. J. Pharm. Sci.

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Sunset yellow (SY), allura red (AR) and fast green (FG) are frequently used in commercial food products, although they are considered to be hazardous to public health due to their toxic efficacy and high exposure risk potency. In this study, a new, rapid, and reliable method based on a magnetic solid-phase extraction (MSPE) was developed for the simultaneous determination of SY, AR, and FG. Fe 3 O 4 modified with Elaeagnus angustifolia was used for the first time as an adsorbent (Fe 3 O 4 -EA) in MSPE. It was characterized with scanning electron microscopy, Brunauer Emmet Teller surface area analysis and X-ray diffraction. MSPE parameters were optimized in terms of pH, adsorption, and elution time and elution volume. High-performance liquid chromatography was used for dye quantitation. Analytical separation was performed by applying ammonium acetate buffer, acetonitrile, and methanol as the mobile phase to a C 18 reverse-phase analytical column. Intraday and inter-day repeatability of the method performed at the concentration of 0.2, 1.0 and 2.0 µg/mL exhibited <8.1% RSD (n=3). The limit of detection values was between 0.05-0.1 µg/mL. The adsorption data of SY, AR and FG on Fe 3 O 4 -EA were fitted with the Langmuir model with q max values of 45.0, 70.4 and 73.0 mg/g, respectively.

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Reversed‐Phase High‐Performance Liquid Chromatographic Method for the Determination of Permaton Red (D&C Red No. 36) in Cosmetics

Cited by 3 publications

References 11 publications

Simultaneous Determination of Cysteamine and Cystamine in Cosmetics by Ion-Pairing Reversed-Phase High-Performance Liquid Chromatography

Simultaneous Determination of Cysteamine and Cystamine in Cosmetics by Ion-Pairing Reversed-Phase High-Performance Liquid Chromatography

Determination of synthetic colorants in cosmetic products by reversed-phase high-performance liquid chromatography coupled with diode-array detector

Determination of sunset yellow, allura red, and fast green using a novel magnetic nanoadsorbent modified with Elaeagnus angustifolia based on magnetic solid-phase extraction by HPLC

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