Reversed-phase high-performance liquid chromatography for the determination of β-asarone

Micali, Giuseppe; Currò, Paolina; Calabrò, Giuseppe

doi:10.1016/s0021-9673(00)87304-5

Cited by 14 publications

(5 citation statements)

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“…Lopez et al (1993) showed that aasarone was highly toxic to rat hepatocytes in uitro. The United States Food and Drug Administration has prohibited the use of the essential oil from Acorus calamus which had been used to flavour vermouths, bitters and liquors (Micali et al, 1980) Despite the potent and broad-spectrum activity shown by the hexane extract of Acorus gramineus it is unlikely that the isolated compounds could ever be of any use in the treatment of parasitic disease due to their toxic and carcinogenic properties. It is worrying that plants containing these carcinogenic asarones are still widely used in traditional medicine in China, India and Mexico and also that in most European countries including Great Britain the presence of @-asarone as a flavouring agent is still permitted in alcoholic beverages up to a concentration of 1 mg/kg (Micah et al, 1980).…”

Section: Discussionmentioning

confidence: 99%

Anthelmintic and pesticidal activity of Acorus gramineus (Araceae) is associated with phenylpropanoid asarones

Perrett

Whitfield

1995

Phytotherapy Research

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The hexane extract of the Chinese medicinal plant Acorus grarnineus (Araceae) has previously been shown to possess significant bioactivity towards a range of organisms in in uitro bioassays including digenean, nematode and arthropod targets. In the present study the principal active components in A. grarnineus were identified as the phenylpropanoids a-and Pasarone by comparing TLC, HPLC, UV and bioassay data obtained using the hexane extract with those derived with commercially available pure compounds. The known mammalian toxicity and carcinogenicity of asarones means that they are unlikely to have any utility as lead compounds for the development of practical anthelmintics and insecticides.

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Section: Discussionmentioning

confidence: 99%

Anthelmintic and pesticidal activity of Acorus gramineus (Araceae) is associated with phenylpropanoid asarones

Perrett

Whitfield

1995

Phytotherapy Research

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“…Numerous high‐performance liquid chromatographic (HPLC) and gas chromatographic–mass spectrometry (GC‐MS) methods have been reported for quantification of BAS from raw materials and commercial formulations (Curro et al ., ; Micali et al ., ; Yu et al ., ). Although various studies have reported the pharmacological action of BAS in recent years, there are limited assays available for the quantification of BAS in biological fluids.…”

Section: Introductionmentioning

confidence: 98%

A rapid HPLC‐ESI‐MS/MS method for determination of β‐asarone, a potential anti‐epileptic agent, in plasma after oral administration of Acorus calamus extract to rats

Nandakumar¹,

Menon

Shailajan³

2012

Biomedical Chromatography

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β-Asarone (BAS), a phenylpropanoid from Acorus calamus Linn., has shown biological effects in the management of cognitive impairment conditions such as Alzheimer's disease. The present paper describes a selective and sensitive liquid chromatography-tandem mass spectrometric method (HPLC-MS/MS) using electrospray ionization source (ESI) for quantification of BAS in rat plasma. Briefly, the plasma samples were pre-treated using a simple solid-phase extraction method. The separation of BAS and the internal standard, caffeine, was achieved on an Agilent Zorbax XDB C(18) column (50 × 2.1 mm i.d., 5 µm) using 0.2 mL/min isocratic mobile phase flow. The detection was performed using an Applied Biosystems Hybrid Q-Trap API 2000 mass spectrometer equipped with an ESI source operated in positive mode. Also, the developed bioanalytical method was validated as per the US FDA bioanalytical guidelines over the concentration range of 9.79-4892.50 ng/mL (r(2) ≥ 0.9951) for BAS from rat plasma. The mean percentage recovery (n = 3) for the low, middle and high quality control samples was 86.92 ± 3.89, 85.30 ± 1.09 and 87.24 ± 4.03%, respectively. The applicability of the validated HPLC-MS/MS method was demonstrated by successful measurement of BAS from plasma following oral administration of Acorus calamus rhizome extracts to three female albino Wistar rats.

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“…Many published methods exist for the determination of RS in finished foods and beverages, of which a selection is given in the References section, but there are only a few that refer to their determination in compound flavourings or their raw materials . The latter mainly concern the analysis in single essential oils of one or two individual RS, and are often based on a direct gas chromatography (GC) or liquid chromatography (LC) analysis.…”

Section: Introductionmentioning

confidence: 99%

Determination of volatile ‘restricted substances’ in flavourings and their volatile raw materials by GC‐MS

Cachet¹,

Brevard²,

Cantergiani³

et al. 2014

Flavour & Fragrance J

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Many flavour regulations around the world contain a list of so‐called ‘restricted substances’ (RS), i.e. substances that occur naturally in source materials for flavourings and food ingredients with flavouring properties, but whose presence in certain foods is restricted and/or for which maximum levels are set, for example, the European regulation 1334/2008. Only a few publications refer to the determination of RS in compound flavourings or their raw materials, and the latter only concern the analysis of one or two individual RS in single essential oils. The Working Group on Methods of Analysis of the International Organization of the Flavor Industry (IOFI) has developed a method for the rapid routine determination of β‐asarone, coumarin, menthofuran, methylchavicol, methyleugenol, pulegone, safrole and α‐ and β‐thujones in flavourings and their raw materials by gas chromatography‐mass spectrometry (GC‐MS), using selected‐ion monitoring and internal standards. The method has been evaluated by nine flavour‐industry laboratories using a complex surrogate flavouring containing all of the above analytes, at concentrations that would be likely to produce levels in finished foods of around typical maximum limits for these RS. Results were obtained from a total of 15 columns and sets of analytical conditions, using 11 GC/MS instruments, with in each case a determination of the analyte in two versions of the flavouring. With reproducibility relative standard deviations (RSDR) of less than about 20%, and recoveries of 80–120%, the method performance can be considered as satisfactory for rapid routine checks on the levels of restricted substances in compound flavourings. The method is intended for flavour‐industry laboratories in order for them to fulfil their obligation to inform food‐industry clients of the amounts of these substances in commercial flavourings, but is not intended for their analysis in finished foods. Copyright © 2014 John Wiley & Sons, Ltd.

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Reversed-phase high-performance liquid chromatography for the determination of β-asarone

Cited by 14 publications

References 1 publication

Anthelmintic and pesticidal activity of Acorus gramineus (Araceae) is associated with phenylpropanoid asarones

Anthelmintic and pesticidal activity of Acorus gramineus (Araceae) is associated with phenylpropanoid asarones

A rapid HPLC‐ESI‐MS/MS method for determination of β‐asarone, a potential anti‐epileptic agent, in plasma after oral administration of Acorus calamus extract to rats

Determination of volatile ‘restricted substances’ in flavourings and their volatile raw materials by GC‐MS

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