Reversed‐Phase HPLC Determination of Sibutramine Hydrochloride in the Presence of its Oxidatively‐Induced Degradation Products

Development and validation of an analytical method by RP-HPLC for quantification of sibutramine hydrochloride in pharmaceutical capsules

“…19 For applications in pharmaceutical products, there are methods that make use of spectrophotometry [20][21][22] and HPLC. 21,[23][24][25][26][27] Singh and coworkers 21 proposed an enantiomeric separation and a quantitative HPLC method for analysis of sibutramine HCl. The enantiomeric separation was obtained on an a-1-acid glycoprotein (chiral-AGP) column in less than 5 min.…”

“…However, the elution time was longer (approximately 15 min). The method described by Segall and coworkers 24 for the determination of sibutramine HCl in the presence of its oxidatively induced degradation products employed a reversed-phase C18 column (Microsorb-MV) at 7 min running time. Chandorkar and coworkers 25 achieved a separation of sibutramine HCl and its impurity in bulk as well as formulation using a stainless steel Lichrosphere C-18 in about 5 min.…”

Sibutramine Selective Electrodes for Batch and Flow Injection Determinations in Pharmaceutical Preparations

“…It is an orally administered agent for treatment of obesity, as an appetite suppressant; it is a centrally acting serotonin-norepinephine reuptake inhibitor structurally related to amphetamines. 1,2 Limited methods of determination of this drug have been reported in the literature, including high performance liquid chromatographic (HPLC), [3][4][5][6] and spectrophotometric [7][8][9][10] and derivative fluorescence spectroscopic 11 methods.…”