Reversed-phase liquid chromatographic separation and simultaneous profiling of steroidal glycoalkaloids and their aglycones

Hybrids and allied species as potential rootstocks for eggplant: Effect of grafting on vigour, yield and overall fruit quality traits

“…Glycoalkaloid extraction was performed from 0.5 g samples of lyophilized and powdered flesh tissue by 95% ethanol. The analyses were carried out by means of RP-HPLC according to Kuronen et al (1999) using partially purified solasonine and solamargine as the external standard. The data were expressed as mg/100 g dw; the limit of detection was 0.03 mg100 g −1 of dw.…”

Cooking influence on physico-chemical fruit characteristics of eggplant (Solanum melongena L.)

“…The glycoalkaloids, solamargine and solasonine, were extracted from 0.5 g of lyophilised tissue using 95% ethanol as described by Birner (1969) with some modifications, and analyzed by means of RP-HPLC according to Kuronen, Väänänen, and Pehu (1999) with minor modifications, using purified solasonine and solamargine as the external standards. The data were expressed as the sum of the two main glycoalkaloids (SGA) of eggplant by mg/100 g of DM.…”

Development of Practical HPLC Methods for the Separation and Determination of Eggplant Steroidal Glycoalkaloids and their Aglycones

“…The final method developed for extraction of glycoalkaloids from eggplant material was based on modification and consideration of the varied procedures as applied to the potato by Kuronen et al [27] Saito, [29] Friedman and Levin, [30] Dao and Friedman, [33] Sotelo and Serrano, [36] Friedman et al, [39] and as applied to Solanum sycophanta by Usubillaga et al [55] Analogous to classical precipitation/crystallization procedures for the extraction of solanine from potato, [56] initial attempts were made to extract the glycoalkaloids from eggplant by prescribed methods that involved collection of either a residue [36] or a precipitate. [39] However, such routes were abandoned because acceptable collection of a precipitate/residue of suitable purity and accurately measured mass was either not possible or not feasible, especially when considering that the final method would eventually be applied to a large set of samples.…”

“…[18 -26] Descriptions of the chemical structures for these similar compounds are found in the literature. [3 -5] Due to the difficulty in the practical separation of all these compounds for rapid analysis, [27] sometimes researchers in this field base their determinations solely on the hydrolysis products and not the actual glycoalkaloids (SGAs); moreover, the aglycones can further hydrolyze, making such determinations of aglycone "equivalents" inaccurate. [28] Methods for the analysis of glycoalkaloids have primarily focused on the potato.…”