Reversed-Phase Liquid Chromatographic Separation of Enantiomeric and Diastereomeric Bases Related to Chloramphenicol and Thiamphenicol

Simultaneous analysis of thiamphenicol and its prodrug thiamphenicol glycinate in human plasma and urine by high performance liquid chromatography: Application to pharmacokinetic study

“…Due to the poor peak symmetry obtained for TG using the chromatographic conditions reported in references [3,[5][6][7] described for TAP appropriate quantities of tetrabutyl ammonium bromide and ammonium acetate were added to the eluent. This improved the peak shape dramatically and enabled complete separation of the analytes and I.S.…”

Separation and preparative purification ofl‐ andd‐valine ester: Diastereomeric conjugates of atazanavir using a combination of 2‐propanol and acetonitrile in reversed‐phase high‐performance liquid chromatography

“…While the separation of enantiomers is usually achieved via the formation of a diastereomeric complex involving differential chiral interactions at the chiral stationary phase, diastereomers can mostly be separated using a chiral chromatography due to differences in physical and chemical properties [1,[3][4][5]. Enantiomers are usually separated by a chiral column in normal-phase liquid chromatography (NPLC) [6][7][8][9][10], reversed-phase liquid chromatography (RPLC) [11][12][13][14][15][16] or supercritical fluid chromatography (SFC) [17][18][19]. Another common approach for the separation of enantiomers by reversed-phase HPLC on achiral stationary phases (e.g., C8, C18) is to convert them into diastereomers by coupling with chiral derivatizing reagents (CDRs) [20].…”

Chiral high‐performance liquid and supercritical fluid chromatographic enantioseparations of limonene‐based bicyclic aminoalcohols and aminodiols on polysaccharide‐based chiral stationary phases

“…High-performance liquid chromatographic (HPLC) methods for enantioresolution include indirect and direct methods. For the enantioseparation of chiral 1,3aminoalcohols indirect methods involve precolumn derivatization with a chiral reagent, with subsequent separation of the diastereoisomers on an achiral column (Gal & Meyer-Lehnert, 1988;Péter et al, 1998;Péter, Kámán, Fülöp, Van der Eycken, & Armstrong, 2001). Direct methods for 1,3-aminoalcohols and aminodiols involve chiral stationary phases (CSPs) based on α 1 -acid glycoprotein (Delée et al, 1987), cellulose tris(3,5)-dimethylphenylcarbamate (Péter et al, 1998;Smith et al, 2010), ß-cyclodextrin (Péter et al, 2001) and Pirkle-type (Hongmei, Xiuzhu, Chunxiu, Baochun, & Xuejun, 2005) columns.…”