Reversed-phase separation of ionic organoborate clusters by high-performance liquid chromatography

The Chemistry of Neutron Capture Therapy

“…A complete assessment of various methods for boron compound detection and analysis is beyond the scope of this review. However, a cursory examination is important since aspects in the synthesis and purification of the compounds relate to one's ability to detect them and to determine, at least in a qualitative way, the degree of compound purity (i.e., by thin-layer chromatography (TLC) 596,597 and by HPLC 598,599 ). The common feature in all of these compounds is, of course, the boron atom and/or various boron-containing complexes.…”

Fragmentation of B12H11S-R(2−) in electrospray mass spectrometry

“…IR (cm À1 ) spectra were determined as KBr disc on a Biorad FTS-7 spectrometer. HPLC was carried out on a RP-18 LiChroCart 125 · 4 mm column [3] with 57% MeOH/43% H 2 O 10 mM tetrabutylammonium hydrogen sulfate pH 7.0 as mobile phase. Detection was at 220 nm.…”

Interaction of organic compounds and boron clusters with new silica matrices containing the phosphatidylcholine headgroup

“…We had previously studied the retention of boron clusters on reversed phase gels in mixed solvents in the presence of ion pair reagents. 19 On RP18, elution in the absence of an organic solvent (methanol, or acetonitrile) was not possible, pointing to a strong interaction of boron cluster compounds with silica gels substituted with hydrophobic chains. The gel pairs SPH and SPHH, as well as SH and SHH, allow to study the reversed phase contribution to retention, as the SPHH and SHH both have a hexyl spacer, which is absent in SPH and SH.…”

“…Chromatographic methods have been applied for investigating the retention characteristics of ionic boron clusters using different matrices, including DEAE-cellulose, 1 hydroxymethyl metacrylate, 2 and RP18 reverse phase material. 3,4 Recently, we have found that ionic boron clusters present an unusually strong retention on carbohydrate-containing matrices such as Superdex, Sepharose, and Sephadex. 5 This phenomenon was unexpected, and it precluded to investigate the interaction of these clusters with liposomes immobilized in Superdex, which had been suggested before as a suitable method to study interactions between drugs and lipids.…”