Reversibility versus Persistence of GPIIb/IIIa Blocker-Induced Conformational Change of GPIIb/IIIa (α_IIbβ₃, CD41/CD61)

A contrast agent recognizing activated platelets reveals murine cerebral malaria pathology undetectable by conventional MRI

“…The mAb anti-LIBS 145 binds to GPIIb/IIIa only in its active conformation and demonstrates strong binding to ADP-activated platelets in the presence of fibrinogen. Generation of anti-LIBS 145 has been previously described in detail (36). In brief, the mAb anti-LIBS 145-expressing hybridoma cell line was used as the basis for the cloning of an anti-LIBS single-chain antibody, scFv.…”

Magnetic Resonance Imaging Contrast Agent Targeted Toward Activated Platelets Allows In Vivo Detection of Thrombosis and Monitoring of Thrombolysis

“…16 Here, we report the generation and application of an MRI contrast agent consisting of MPIOs and a single-chain antibody that selectively binds to ligand-induced binding sites (LIBS) on the activated platelet integrin GPIIb/IIIa. 17 We show the utility of this contrast agent in rapid identification of mural platelet-containing thrombi in vivo using a mouse model of wall-adherent carotid thrombosis. Pharmacological thrombolysis was used to further evaluate whether LIBS MPIO-induced signal void intensity reports reliably on thrombus size, particularly size reduction.…”

Dual-Contrast Molecular Imaging Allows Noninvasive Characterization of Myocardial Ischemia/Reperfusion Injury After Coronary Vessel Occlusion in Mice by Magnetic Resonance Imaging

“…Generation and purification of the antibody were performed as described elsewhere. 15,19,21 For construction of the contrast agent, cobalt-functionalized autofluorescent MPIOs with a diameter of 1μm were conjugated to the histidine tag of the anti-LIBS/control single-chain antibody as described in the manufacturer's protocol (Dynal Biotech, Oslo, Norway) and in previously published studies. [22][23][24] Throughout this article, MPIOs conjugated to the anti-LIBS antibody are referred to as LIBS-MPIOs, and MPIOs conjugated to control antibody are called control-MPIOs.…”

“…19 The cloning, generation, and production of the anti-LIBS single-chain antibody have previously been described in detail. 20 To obtain a nonfunctional antibody for control purposes, exchange of the arginine in the RXD motif of the heavychain CDR3 region of a single-chain antibody was performed.…”