Reversible changes of extracellular pH during action potential generation in a higher plant Cucurbita pepo

Vodeneev, Vladimir; Opritov, V. A.; Pyatygin, S. S.

doi:10.1134/s102144370604008x

Cited by 44 publications

(39 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In seems that AP and VP are different in their ionic nature and propagation mechanisms. An actively propagating AP [4] is associated mainly with Ca 2+ , Cl -, and K + flows [1, 3, 5, 6], although a reversible inactivation of H + ATPase definitely con tributes to its generation [6]. For VP, the major mecha nism is a decrease in H + ATPase activity [3,4,7] that seems to be induced by entrance of calcium ions [8,9].…”

mentioning

confidence: 99%

Participation of intracellular and extracellular pH changes in photosynthetic response development induced by variation potential in pumpkin seedlings

Sherstneva

Vodeneev

Katicheva

et al. 2015

Biochemistry Moscow

Self Cite

View full text Add to dashboard Cite

Electrical signals presented in plants by action potential and by variation potential (VP) can induce a reversible inactivation of photosynthesis. Changes in the intracellular and extracellular pH during VP generation are a potential mechanism of photosynthetic response induction; however, this hypothesis requires additional experimental investigation. The purpose of the present work was to analyze the influence of pH changes on induction of the photosynthetic response in pumpkin. It was shown that a burning of the cotyledon induced VP propagation into true leaves of pumpkin seedlings inducing a decrease in the photosynthetic CO2 assimilation and an increase in non-photochemical quenching of fluorescence, whereas respiration was activated insignificantly. The photosynthetic response magnitude depended linearly on the VP amplitude. The intracellular and extracellular concentrations of protons were analyzed using pH-sensitive fluorescent probes, and the VP generation was shown to be accompanied by apoplast alkalization (0.4 pH unit) and cytoplasm acidification (0.3 pH unit). The influence of changes in the incubation medium pH on the non-photochemical quenching of fluorescence of isolated chloroplasts was also investigated. It was found that acidification of the medium stimulated the non-photochemical quenching, and the magnitude of this increase depended on the decrease in pH. Our results confirm the contribution of changes in intracellular and extracellular pH to induction of the photosynthetic response caused by VP. Possible mechanisms of the influence of pH changes on photosynthesis are discussed.

show abstract

mentioning

confidence: 99%

Participation of intracellular and extracellular pH changes in photosynthetic response development induced by variation potential in pumpkin seedlings

Sherstneva

Vodeneev

Katicheva

et al. 2015

Biochemistry Moscow

Self Cite

View full text Add to dashboard Cite

show abstract

“…AP generation is mainly based on passive Ca 2C , Cl ¡ and K C fluxes, 35 but transient H C -ATPase inactivation also participates in this process. 36 VP generation is still under investigation 7,[11][12][13] and it has been difficult to determine its precise functional role. AP is subjected to the 'all-or-none law'; i.e., propagation of this signal isn't directly related to its functional role.…”

Section: Introductionmentioning

confidence: 99%

Variation potential in higher plants: Mechanisms of generation and propagation

Vodeneev

Akinchits

Sukhov

2015

Plant Signaling & Behavior

104

158

View full text Add to dashboard Cite

“…Recently, Vodeneev et al (2006) reported extracellular pH transients during action potential generation in Cucurbita pepo following gradual cooling from 26-28°C to 8-10°C. Authors found that the pH kinetics corresponded with the AP with a slightly delayed pH peak and argued that the depolarization was due to a temporarily deactivated H + pump and the pH change a result of H + inXux.…”

Section: The Sequence Of Eventsmentioning

confidence: 99%

Dissection of heat-induced systemic signals: superiority of ion fluxes to voltage changes in substomatal cavities

Zimmermann

Felle

2008

Planta

View full text Add to dashboard Cite

Using non-invasive ion-selective microprobes, that were placed in substomatal cavities, long-distance signalling has been investigated in intact Hordeum vulgare and Vicia faba seedlings. Heat (flame), applied to one leaf (S-leaf), triggers apoplastic ion activity (pH, pCa, pCl) transients in a distant leaf (T-leaf), all largely independent of simultaneously occurring action potential-like voltage changes. While apoplastic pCa and pH increase (Ca(2+)-, H(+)-activities decrease), pCl decreases (Cl(-)-activity increases). As the signal transfer from the S- to the T-leaf is too fast to account for mass flow, the heat-induced pressure change is primarily responsible for changes in voltage (H(+) pump deactivation) as well as for the ion fluxes. The pCa transient precedes the pCl- and pH responses, but not the voltage change. Since the apoplastic pCl decrease (Cl(-) increase) occurs after the pCa increase (Ca(2+) decrease) and after the depolarization, we argue that the Cl(-) efflux is a consequence of the Ca(2+) response, but has no part in the depolarization. Kinetic analysis reveals that pH- and pCl changes are interrelated, indicated by the action of the anion channel antagonist NPPB, which inhibits both pCl- and pH changes. It is suggested that efflux of organic anions into the apoplast causes the pH increase rather than the deactivation of the plasma membrane H(+) pump. Since there is considerably more information in ion activity changes than in a single action- or variation potential and heat-induced ion fluxes occur more reliably than voltage changes, released by milder stimuli, they are considered systemic signalling components superior to voltage.

show abstract

Reversible changes of extracellular pH during action potential generation in a higher plant Cucurbita pepo

Cited by 44 publications

References 13 publications

Participation of intracellular and extracellular pH changes in photosynthetic response development induced by variation potential in pumpkin seedlings

Participation of intracellular and extracellular pH changes in photosynthetic response development induced by variation potential in pumpkin seedlings

Variation potential in higher plants: Mechanisms of generation and propagation

Dissection of heat-induced systemic signals: superiority of ion fluxes to voltage changes in substomatal cavities

Contact Info

Product

Resources

About