Reversible Conjugation of Gibberellins <i>In Situ</i> in Maize

Rood, Stewart B.; Pharis, Richard P.; Koshioka, Masaji

doi:10.1104/pp.73.2.340

Cited by 59 publications

(26 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This graded response to increasing levels of GA3 suggests that gibberellin was limiting in kernels which have not been dried. Rood et al (21) found that mature seeds contain predominantly glucosyl conjugates 6 HAI 010…”

Section: Discussionmentioning

confidence: 99%

Distinction between the Responses of Developing Maize Kernels to Fluridone and Desiccation in Relation to Germinability, α-Amylase Activity, and Abscisic Acid Content

Oishi¹,

Bewley²

1990

Plant Physiol.

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Distinction between the Responses of Developing Maize Kernels to Fluridone and Desiccation in Relation to Germinability, α-Amylase Activity, and Abscisic Acid Content

Oishi¹,

Bewley²

1990

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…SiO, partition chromatography was performed on each extract to further separate free GAs from GA conjugates (adapted from Rood et al, 1983). Briefly, the samples were dissolved in 0.5 mL of methano1:ethyl acetate:H,O (50:50:1, v/v) and placed at the top of a SiO, column (1 g; 20% H,O by weight) which had been slurried and packed with formic acid-saturated hexane:ethyl acetate (95:5, v/v).…”

Section: Chromatographymentioning

confidence: 99%

Seed and 4-Chloroindole-3-Acetic Acid Regulation of Gibberellin Metabolism in Pea Pericarp

et al. 1995

View full text Add to dashboard Cite

show abstract

“…Water in the EtOAc-soluble extract was removed by freezing and filtration of ice, and the EtOAc was taken to dryness in vacuo at 35°C. After freeze-drying, the extract residue was dissolved in MeOH:EtOAc (1:1) and loaded onto glass-fiber filter paper discs prior to stepwiseelution SiO2 partition column chromatography (3,21). The resulting 26 fractions were bioassayed at 1/200 and 1/400 dilutions using a modified (19) Tan-ginbozu dwarf rice microdrop assay (13).…”

Section: Analysis Of Gibberellin-like Substancesmentioning

confidence: 99%

Endogenous Gibberellins and Shoot Growth and Development in Brassica napus

Rood¹,

Mandel²,

Pharis³

1989

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

Greenhouse-grown oilseed rape (Brasskca napus, annual Canola variety 'Westar') plants were harvested at six dates from the vegetative phase until the early pod (silique)-fill/late flowering stage. Endogenous gibberellin (GA)-like substances were extracted from stems, purified, and chromatographed on silica gel partition columns prior to bioassay in serial dilution using the 'Tan-ginbozu' dwarf rice microdrop assay. The concentrations of total endogenous GA-like substances were low during vegetative stages (1 nanogram GA3 equivalents/gram dry weight), and rose 300-fold by the time of floral initiation. After floral initiation the concentration of GA-like substances fell, then rose again during bolting to maximal levels during the early pod-fill stage (940 nanograms per gram dry weight). The qualitative profiles of GAlike substances varied across harvests, with higher proportions of a GAr-like substance at the early pod-fill stage. In a second study stems were similarly harvested at eight dates and the concentrations of endogenous GA1, the principal bioactive native GA of oilseed rape, were determined by gas chromatographyselected ion monitoring using [17,17-2H]GAI as a quantitative intemal standard. The concentration of GA1 increased at about the time of floral initiation and then subsequently fell, thus confirming the pattem noted above for total GA-like substances. The exogenous application of paclobutrazol (PP333), a persistent triazole plant growth regulator (PGR) which blocks GA biosynthesis, or another triazole, triapenthenol (RSW0411), prevented flowering as well as bolting; plants remained at the vegetative rosette stage. These results imply a causal role for endogenous GA, in the control of bolting, which normally precedes anthesis. Further, the rise in the concentration of total endogenous GA-like substances, including GA,, which was associated with floral initiation, and the prevention of visable floral development by the triazole PGRs, also indicates a role for endogenous GAs in the regulation of flowering in B. napus.

show abstract

Reversible Conjugation of Gibberellins In Situ in Maize

Cited by 59 publications

References 24 publications

Distinction between the Responses of Developing Maize Kernels to Fluridone and Desiccation in Relation to Germinability, α-Amylase Activity, and Abscisic Acid Content

Distinction between the Responses of Developing Maize Kernels to Fluridone and Desiccation in Relation to Germinability, α-Amylase Activity, and Abscisic Acid Content

Seed and 4-Chloroindole-3-Acetic Acid Regulation of Gibberellin Metabolism in Pea Pericarp

Endogenous Gibberellins and Shoot Growth and Development in Brassica napus

Contact Info

Product

Resources

About