Reversible desensitization of the myocardial contractile apparatus for calcium *1A new concept for improving tolerance to cold ischemia in human myocardium?

Abstract

I) BDM reduced the isometric force development of the electrically driven isolated human myocardial muscle strip in a dose-dependent way.(ABSTRACT TRUNCATED AT 250 WORDS)

Improved right heart function after myocardial preservation with 2,3-butanedione 2-monoxime in a porcine model of allogenic heart transplantation

“…In contrast to these theoretical considerations, there is strong evidence from various experimental settings that adjunctive application of BDM to cardioplegic solutions is beneficial. 4,10,11 Contraction uncoupling during initial postischemic reperfusion by BDM has been equally effective when applied over a 20-minute period in a dosage of 20 mmol/L. 9 The drug is fast acting and its effects are fully reversible within minutes.…”

“…Two control groups received either UW or Bretschneider's HTK solution as the cardioplegic solution. One group received BDM in Krebs-Henseleit buffer (30 mmol/L), 10,11 and one group received BDM in HTK solution (30 mmol/L). The solutions were of the following compositions:…”

Evidence for a negative inotropic effect of obesity in human myocardium?☆

“…BDM was used to reduce the 'cutting injury' of excised myocardial tissue and to improve the tolerance towards ischemia [7]. From any auricle two trabeculae were obtained (diameter: 0.5 mm, length: 5-6 mm).…”

“Cardioplegia on the Contractile Apparatus Level”: Evaluation of a New Concept for Myocardial Preservation in Perfused Pig Hearts

“…Both types are designed to reduce myocardial energy demand during ischemia in order to delay the development of irreversible myocardial injury (1,4,11,25). Recently, reversible desensitization of the myocardial contractile apparatus for calcium had been suggested as a third concept for myocardial protection and was subsequently analyzed, based an experiments with rabbit and mice hearts ( 32,37 ) and isolated human atrial and ventricular tissue (2,28,36 ). Supposing that the myocardial energy demand during ischemia is directly related Thorac.…”

“…A compound known to have these properties is 2,3 Butanedione monoxime (BDM) (8,10,15,20,28,36,37). Simultaneous recordings of isometric force development and intracellular calcium transient in the presence of various concentrations of BDM gave direct evidence that the contractile apparatus of human myocardium can be completely inactivated by this compound as indicated by the complete absence of mechanical responses to electrical stimulation despite intracellular calcium transients which were sufficiently high to elicit contractions (36). Remarkable improvements of the duration of cold storage periods were observed when this compound was used in isolated human muscle strips and compared to Bretschneider's cardioplegic solution (HTK) (2, 36).…”